Intraosseous infusion of liposome‐encapsulated hemoglobin (HbV) acutely prevents hemorrhagic anemia‐induced lethal arrhythmias, and its efficacy persists with preventing proarrhythmic side effects in the subacute phase of severe hemodilution model

Takase, Bonpei; Higashimura, Yuko; Asahina, Haruka; Masaki, Nobuyuki; Kinoshita, Manabu; Sakai, Hiromi

doi:10.1111/aor.14170

Cited by 4 publications

(1 citation statement)

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“…HbV, due to its smaller size, is better than red blood cells for reperfusion into the capillaries of the graft and helps to oxygenate the graft tissue . Recent studies have shown that liposome-encapsulated hemoglobin can improve the arrhythmia in rabbit obstetric hemorrhage and severe fatal hemorrhage models . HbV-based oxygen carriers can also be used as organ supports, stabilizing the circulation of pneumonectomy rats with hemorrhagic shock .…”

Section: Hb-based Oxygen Carriersmentioning

confidence: 99%

Research Progress in Oxygen Carrier Design and Application

Ye,

Zheng,

Chen

et al. 2023

Mol. Pharmaceutics

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Ischemia or hypoxia can lead to pathological changes in the metabolism and function of tissues and then lead to various diseases. Timely and effective blood resuscitation or improvement of hypoxia is very important for the treatment of diseases. However, there is a need to develop stable, nontoxic, and immunologically inert oxygen carriers due to limitations such as blood shortages, different blood types, and the risk of transmitting infections. With the development of various technologies, oxygen carriers based on hemoglobin and perfluorocarbon have been widely studied in recent years. This paper reviews the development and application of hemoglobin and perfluorocarbon oxygen carriers. The design of oxygen carriers was analyzed, and their application as blood substitutes or oxygen carriers in various hypoxic diseases was discussed. Finally, the characteristics and future research of ideal oxygen carriers were prospected to provide reference for follow-up research.

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Section: Hb-based Oxygen Carriersmentioning

confidence: 99%

Research Progress in Oxygen Carrier Design and Application

Ye,

Zheng,

Chen

et al. 2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

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Liposome‐encapsulated hemoglobin rescues hemorrhagic shock heart through anti‐ischemic and anti‐arrhythmogenic effects on myocardium in repetitive 65% bleeding rat model

Takase,

Higashimura,

Masaki

et al. 2024

Artificial Organs

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BackgroundPhase I clinical trial of an artificial oxygen carrier (liposome‐encapsulated hemoglobin vesicles [HbVs]) is safely completed, considering the other clinical application. Herein, we aimed to investigate the resuscitation effects of HbVs in cases of lethal hemorrhage, including the mechanisms involved.MethodsOptical mapping analysis (OMP) and electrophysiological studies (EPS), immunostaining pathological examination for hypoxia‐inducible factor‐1α (HIF1‐alpha) in the heart tissue, and blood troponin I (TnI) level measurements were performed in rats that underwent five rounds of spontaneous arterial bleeding with up to 65% hemorrhage.ResultsTen rats in each group were resuscitated by a transvenous infusion of 5% albumin (ALB), washed erythrocytes (wRBC), HbVs (HbV), 50% HbV diluted by 5% albumin (50% HbV), and 66% HbV diluted by 5% albumin (66% HbV). The rats in the ALB and 50% HbV groups died, whereas those in the other groups survived. OMP showed impaired action potential duration dispersion (APDd) in the left ventricle in the ALB and 50% HbV groups, which was attenuated in the other groups. Lethal arrhythmias were provoked by EPS in the ALB and 50% HbV groups but not in the other groups. HIF1‐alpha was positively stained only in the ALB and 50% HbV groups. TnI levels were elevated in the ALB and 50% HbV groups.ConclusionsAcute lethal hemorrhage causes myocardial ischemia with hypoxia and arrhythmias, which may be induced by impaired APDd and myocardial damage, reflected in the increased levels of HIF1‐alpha and TnI. HbVs could be useful for resuscitation and may help save patients with injuries such as gunshot wounds.

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