Intraoperative serum lactate is not a predictor of survival after glioblastoma surgery

Cata, Juan P.; Bhavsar, Shreyas; Hagan, Katherine; Arunkumar, Radha; Grasu, Roxana; Dang, Anh; Carlson, Richard; Arnold, Benjamin; Popat, Keyuri; Rao, Ganesh; Potylchansky, Y.; Lipski, Ian; Ratty, Sally; Nguyen, Anh T.; McHugh, Thomas M.; Feng, Lei; Rahlfs, Thomas F.

doi:10.1016/j.jocn.2017.05.004

Cited by 14 publications

(17 citation statements)

References 20 publications

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“…However, a recent report by Vietheer et al demonstrated that serum GFAP levels do not correlate with tumor recurrence in GBM in 33 samples in their studies [ 17 ]. Similar results were also observed for another protein, lactate, in serum [ 15 ]. Very few studies have been done on lncRNAs as biomarker in GBM.…”

Section: Resultssupporting

confidence: 87%

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Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

et al. 2018

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Glioblastoma multiforme (GBM) is the most common and aggressive malignant adult primary brain tumor. Despite surgical resection followed by radiotherapy and chemotherapy, the median survival rate is approximately 14 months. Although experimental therapies are in clinical trials for GBM, there is an urgent need for a peripheral GBM biomarker for measuring treatment response. As we have previously demonstrated that the long noncoding RNA HOX Transcript Antisense Intergenic RNA, or HOTAIR, is dysregulated in GBM and required for GBM cell proliferation, we hypothesized that HOTAIR expression may be utilized as a peripheral biomarker for GBM. HOTAIR expression was measured in serum from 43 GBM and 40 controls using quantitative real-time PCR (qRT-PCR). The PCR products were subsequently subcloned into pCR™4-TOPO®TA vectors for DNA sequencing. A ROC curve was also generated to examine HOTAIR’s prognostic value. The amount of HOTAIR in serum exosomes and exosome-depleted supernatant was calculated by qRT-PCR. The relative HOTAIR expression was also investigated in 15 pairs of GBM serum and tumors. We detected HOTAIR in serum from GBM patients. HOTAIR levels in serum samples from GBM patients was significantly higher than in the corresponding controls (P < 0.0001). The area under the ROC curve distinguishing GBM patients from controls was 0.913 (95% CI: 0.845–0.982, P < 0.0001), with 86.1% sensitivity and 87.5% specificity at the cut-off value of 10.8. HOTAIR expression was significantly correlated with high grade brain tumors. In addition, Pearson correlation analysis indicated a medium correlation of serum HOTAIR levels and the corresponding tumor HOTAIR levels (r = 0.734, P < 0.01). We confirmed via sequencing that the amplified HOTAIR from serum contained the HOTAIR sequence and maps to the known HOTAIR locus at 12q13. The serum-derived exosomes contain HOTAIR and the purified exosomes were validated by western blot and nanoparticle tracking analysis. Importantly, our results demonstrate that serum HOTAIR can be used as a novel prognostic and diagnostic biomarker for GBM.Electronic supplementary materialThe online version of this article (10.1186/s12943-018-0822-0) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 87%

“…Several proteins and microRNAs such as GFAP, lactate, miR-504, and miR-137 have been previously described as possible biomarkers for GBM [ 15 – 17 ]. However, a recent report by Vietheer et al demonstrated that serum GFAP levels do not correlate with tumor recurrence in GBM in 33 samples in their studies [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

et al. 2018

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“…e research was conducted up to April 2019 and yielded 215 studies. e articles [1][2][3]5,9,14] in which the correlation between the level of serum lactate and intracranial tumors was studied are reported in Table 4. In an initial series of 18 patients submitted to craniotomy for intracranial tumor, the authors found an increase of serum lactate during surgery regardless to the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…is is an important topic to address in future although the previous studies failed to demonstrate a correlation between increased serum lactate and patient's prognosis. [2,3,14]…”

Section: Discussionmentioning

confidence: 99%

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Factors affecting serum lactate in patients with intracranial tumors – A report of our series and review of the literature

Ioannoni

Grande

Olivi

et al. 2020

Surgical Neurology International

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Background: A hyperlactemia may occur in the presence of tissue hypoperfusion, in diseases affecting metabolism and in cases of malignant neoplasm. However, the factors affecting the serum lactate levels in patients submitted to craniotomy for the resection of an intracranial tumor have been investigated only marginally. Here, we assessed the factors possibly affecting the levels of serum lactate in intracranial tumors and carried out a thorough literature review on this topic. Methods: All patients submitted to elective craniotomy from January 2017 to August 2018 for the resection of a glioblastoma (GBM; 101 cases) and a benign meningioma (WHO I; 105 cases) were included in this study. The sex, age, histological diagnosis, body mass index (BMI), and diabetes were assessed as possible factors affecting the level of the preoperative and postoperative serum lactate in these patients. Results: We found that preoperative hyperlactemia (> 2 mmol/l) was more frequent in patients with GBM than in patients with meningioma (P = 0.0003). Moreover, a strong correlation between a preoperative lactemia and postoperative lactemia (P < 0.0001) was observed. On univariate analysis, we found increased preoperative serum lactate levels in GBM patients (P = 0.0022) and in patients with a BMI ≥30 (P = 0.0068). Postoperative serum lactate levels were significantly higher in GBM patients (P = 0.0003). On multivariate logistic regression analysis, a diagnosis of GBM was an independent factor for higher level of preoperative (P = 0.0005) and postoperative (P < 0.0001) serum lactate. Conclusion: The malignant phenotype of GBM is the strongest factor associated with a pre- and postoperative hyperlactemia in patients submitted to craniotomy for the resection of an intracranial tumor.

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The Warburg effect in patients with brain tumors: a comprehensive analysis of clinical significance

Ma,

Lee,

et al. 2023

J Neurooncol

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Intraoperative serum lactate is not a predictor of survival after glioblastoma surgery

Cited by 14 publications

References 20 publications

Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

Serum long noncoding RNA HOTAIR as a novel diagnostic and prognostic biomarker in glioblastoma multiforme

Factors affecting serum lactate in patients with intracranial tumors – A report of our series and review of the literature

The Warburg effect in patients with brain tumors: a comprehensive analysis of clinical significance

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