http://www.eje.cz (Fowler et al., 1989). In the last decade, there have been numerous studies on ant parasitoids (Bragança et al., 2021) and other insects associated with ants (Parker, 2016; Rocha et al., 2020), however, most of these focus on aspects of ecology and control (Chen & Fadamiro, 2018 -review).The Neotropical Forest fi re ant Solenopsis virulens was described by Frederick Smith ( 1858), but there are very few studies on its biology. This species belongs to the fi re ant Solenopsis virulens species-group (Pitts et al., 2018) and is more poorly studied than other species of fi re ant (Fox, 2014). Only in the last decade have aspects of its biology and nests been published (Pereira et al., 2015(Pereira et al., , 2021. This ant has been collected almost exclusively in three different forest biomes in Brazil: Atlantic Forest (Resende et al., 2011), Caatinga Dry Forest (Arnan et al., 2018 and Amazonian Forest (Prado et al., 2019). Papers on this species are limited to worker ants captured by traps (Oliveira et al., 2009) or taxonomic studies (Pitts et al., 2018). This is probably related to the fact that colonies of these ants are rarely found compared to other species of fi re ant. Their