Intranasal administration of plasmid DNA-coated nanoparticles results in enhanced immune responses

Abstract

Intranasal immunization offers potential forthe elicitation of effective mucosal and systemic immune responses. In this study, a previously reported novel cationic nanoparticle engineered from a microemulsion precursor was further modified, optimized and applied intranasally to mice to explore its potential as a plasmid DNA (pDNA) vaccine delivery system. To this end, more uniform nanoparticles (around 100 nm) containing less cationic surfactant were developed. The pDNA-coated nanoparticles significantly enhanced the specific serum IgG and IgA titres to an expressed model antigen, beta-galactosidase, by 18-28 and 25-30 fold, respectively, when compared with naked pDNA alone. An enhanced splenocyte proliferative response was also observed after immunization with the pDNA-coated nanoparticles. It was concluded that these plasmid DNA-coated nanoparticles may have potential for immunization via the nasal route.

Preparation, Characterization, Pharmacokinetics and Tissue Distribution of Solid Lipid Nanoparticles Loaded with Tetrandrine

“…A mixture of different surfactants was able to improve the physical characteristics of SLNs (26,27). Combinations of Lipoid E80 and Pluronic F68 were investigated first.…”

The Mucosal Immune System: Modulation by Microemulsion

“…DNA-coated nanoparticles significantly enhanced specific serum IgG and IgA. An enhanced splenocyte proliferative response was also observed after immunisation with pDNA-coated nanoparticles; thus, these particles may be used for immunisation via the nasal route 25 and inhalable nanoparticles may enhance immune responses 25,28 .…”

“…In addition to these advantages, microemulsions are easy to administer and offer several benefits for oral administration. Namely, microemulsions are ideal for the oral and nasal delivery of drugs and vaccines 25,26,27 . Microemulsion techniques are capable of delivering inorganic and organic nano-sized particles with minimal agglomeration because the reaction occurs in nano-sized domains.…”

Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation

“…33,43 In this present preliminary study, the potential application of using a biodegradable, nontoxic copolymer (pHEMA nanoparticles) as a delivery platform to carry pCAG-HAk plasmid DNA has been investigated. In the past, pHEMA has been used in drug delivery systems; 26,31 however, to date, it appears that this study is the first to use pHEMA nanoparticles as an adjuvant in DNA vaccination.…”

“…This vaccine was able to enhance the in-vitro cell transfection efficiency and achieve a substantial cellular immune response (16-200 times greater than the normal plasmid DNA by itself) in mice cells via a number of delivery routes. 4,[31][32][33] Furthermore, it has also been shown that when both the cholera toxin and lipid A were administered with a nanoparticle-based plasmid DNA, there was an overall synergistic effect which enhanced the immune response of the cellular tissues. 5,33 Thus, nanoparticles can also be seen as a novel class of adjuvants, with the potential to be effective delivery platforms for proteins and plasmid DNA immunogens, which can successfully induce a positive immune response.…”