Intramyocardial delivery of human CD133+ cells in a SCID mouse cryoinjury model: Bone marrow vs. cord blood-derived cells☆

Ma, Nan; Ladilov, Yury; Moebius, Jeannette M.; Ong, Lawrence; Piechaczek, Christoph; David, Arpad; Kaminski, Alexander; Choi, Yeong‐Hoon; Li, Wenzhong; Egger, Dietmar; Stamm, Christof; Steinhoff, Gustav

doi:10.1016/j.cardiores.2006.03.020

Cited by 82 publications

(62 citation statements)

References 41 publications

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“…Comparing the in vivo behavior of ASCs and MSCs in the necrotic heart demonstrated that, in general, both populations of stem cells do not tolerate the cardiac environment, resulting in acute donor cell death and a subsequent loss of cardiac function similar to control groups. Nevertheless, some tolerance, although not ideal, was observed with CD133 + MSCs [15]. According to Davy et al [16] enrichment of autologous bone marrow CD34 + stem cells could increase the efficacy of treatments in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy

González‐Garza

Alcaraz²,

Gonzalez-Jara³

et al. 2014

SCD

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Stem cell therapy for cardiac infarct regeneration has been widely used in clinical research. Despite the fact that important advances in this field have been reached, the observed recovery does not demonstrate new cardiac muscle formation. Benefits have been observed due to an improvement in neovascularisation. The main objective of this study was to determine if predifferentiated stem cells into cells of myocardic lineage are capable of engraftment in animal models with induced cardiac infarct and are capable of truly differentiating into myocardiocytes. Bone marrow rat stem cells were predifferentiated with 5-AZ. After 4 weeks, pre-differentiated stem cells express muscarinic 1, 2 and β adrenergic 2 receptors. Also, proteins such as sarcomeric α-actin, cardiac myosin heavy chain, desmin and vimentin were detected by immunocytochemistry. Cells were transplanted intracardialy in an ischemic cardiac rat model. Pre-differentiated or non differentiated cells were transplanted after 4 weeks post infarct induction. Histopathology of the hearts was made 2 weeks after cell transplantation. Typical granulated tissue, scare formation and neovascularisation were observed in both groups. However, in those hearts from rats inoculated with pre-differentiated cells many appeared atypical and were α-actin sarcomeric positive. These events suggest that pre-differentiated cells conserve some muscle characteristic traits in situ that at least last for two weeks after transplantation.

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Section: Introductionmentioning

confidence: 99%

Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy

González‐Garza

Alcaraz²,

Gonzalez-Jara³

et al. 2014

SCD

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“…Of interest, Ma et al compared the effects of cell transplantation between BM stem cells and HUCB stem cells for injured myocardium. HUCB CD133 + cells (5 x 10 5 ) were injected directly into the infarcted myocardium induced by cryoinjury of NOD/SCID mice (Ma et al, 2006a;2006b). An equivalent number of CD133 + BM cells were transplanted and a sham injection was performed in the respective control groups.…”

Section: Intramyocardial Injectionmentioning

confidence: 99%

Human Umbilical Cord Blood Stem Cells Rescue Ischemic Tissues

Park¹,

Lee²,

Eve³

et al. 2011

Advances in Regenerative Medicine

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“…A 2012 meta-analysis of randomized controlled clinical trials concluded intracoronary delivery of BM cells significantly increased functionality and reduced MI reoccurrence [24]. Comparisons of the relative efficacies of different cells types have shown BM mononuclear cells to be more effective than BM mesenchymal stem cells (MSC) [25] and adult HSC to be more effective than cord blood stem cells [26]. However, the relative ability of HSC within WBM cell preparations to facilitate recovery from cardiac damage has not yet been assessed.…”

Section: Introductionmentioning

confidence: 99%

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Davy¹,

Walker²,

Wong³

et al. 2013

SCD

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Recent studies suggest that whole bone marrow (WBM) derived stem cells may facilitate recovery following myocardial infarction. However, the sub-population of WBM responsible for recovery remains uncertain. By adjusting the abundance of CD34 + LinNeg cells in human bone marrow we examined the relative significance of hematopoietic stem cells (HSC) in the recovery of cardiac function in a murine model of induced myocardial infarction. Enrichment of HSC by ~100-fold in WBM transplanted into mice significantly increased recovery of heart function and reduced scar size compared to transplantation of WBM depleted in HSC by ~10-fold (P < 0.05, and P < 0.01 respectively). Peri-infarct capillary density was significantly increased in recipients of HSC-enriched samples (P < 0.01) or WBM samples (P < 0.01) compared to controls. These results strongly suggest a critical role for HSC in the effective treatment of myocardial infarction with human bone marrow, and imply that enrichment of HSC may markedly benefit the clinical application of WBM treatments.

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Intramyocardial delivery of human CD133+ cells in a SCID mouse cryoinjury model: Bone marrow vs. cord blood-derived cells☆

Abstract: While both cord blood and marrow CD133+ cells have some beneficial effects on post-injury angiogenesis and survival, only marrow cells appear to improve myocardial contractility.

Cited by 82 publications

References 41 publications

Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy

Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy

Human Umbilical Cord Blood Stem Cells Rescue Ischemic Tissues

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

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