Intramuscular administration of a DNA vaccine encoding OmpK antigen induces humoral and cellular immune responses in flounder (Paralichthys olivaceus) and improves protection against Vibrio anguillarum

Xu, Hongsen; Xing, Jing; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

doi:10.1016/j.fsi.2018.11.073

Cited by 24 publications

(10 citation statements)

References 49 publications

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“…However, various factors, such as vaccine type, antigen dose, vaccination schedule (with or without booster), vaccine delivery route and the application of adjuvants can influence the vaccine efficacy [95]. Similarly, recent vaccine studies in Vibrio alginolyticus [47,96], Vibrio anguillarum [10,11,97,98], Vibrio harveyi [48], and Edwardsiella tarda [13] indicated that vaccination of recombinant proteins in combination with adjuvants could confer higher RPS levels than immunization with a DNA vaccine alone. Interestingly, pcHrp1, when co-administrated with pcIL-34, could enhance the protection by generating better RPS (82.14%) compared with rHRP1 emulsified with ISA 763 A VG; hence, this result might explain the superiority of IL-34 as an molecular adjuvant.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown that DNA vaccines could be used to control viral pathogens; however, only a few have been tested against bacterial infections. Reportedly, DNA vaccines have been utilized in fish to control Streptococcus iniae [9], Vibrio anguillarum [10,11], Edwardsiella tarda [12][13][14], and Nocardia seriolae [15,16], indicating that DNA vaccines could be suitable for controlling bacterial diseases by provoking both non-specific and specific immune host responses. Despite the advantages of DNA vaccines, including low cost, safety, stability, and efficacy against bacterial and viral diseases, there are only two DNA vaccines licensed for use in commercial aquaculture.…”

Section: Introductionmentioning

confidence: 99%

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Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

2020

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DNA vaccines have been widely employed in controlling viral and bacterial infections in mammals and teleost fish. Co-injection of molecular adjuvants, including chemokines, cytokines, and immune co-stimulatory molecules, is one of the potential strategies used to improve DNA vaccine efficacy. In mammals and teleost fish, interleukin-34 (IL-34) had been described as a multifunctional cytokine and its immunological role had been confirmed; however, the adjuvant capacity of IL-34 remains to be elucidated. In this study, IL-34 was identified in largemouth bass. A recombinant plasmid of IL-34 (pcIL-34) was constructed and co-administered with a DNA vaccine encoding hypoxic response protein 1 (Hrp1; pcHrp1) to evaluate the adjuvant capacity of pcIL-34 against Nocardia seriolae infection. Our results indicated that pcIL-34 co-injected with pcHrp1 not only triggered innate immunity and a specific antibody response, but also enhanced the mRNA expression level of immune-related genes encoding for cytokines, chemokines, and humoral and cell-mediated immunity. Moreover, pcIL-34 enhanced the protection of pcHrp1 against N. seriolae challenge and conferred the relative percent survival of 82.14%. Collectively, IL-34 is a promising adjuvant in a DNA vaccine against nocardiosis in fish.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

2020

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“…In the present study, based on the bioinformatics analysis of amino acid sequences, two 14-aa epitope peptide candidates were identified and employed as immunogens to develop mAbs against flounder CD4-1 and CD4-2. Although polyclonal antibodies against recombinant CD4-1 and CD4-2 proteins of flounder were produced, respectively, the dynamic change in the percentages of CD4 + cells was used as an indicator of the fish health status and vaccine evaluation in our lab [19][20][21][22][23][24][25] . However, some characteristics of polyclonal antibodies make them incomparable to monoclonal antibodies, and mAbs against CD4 molecules are sustainable and powerful tools in the functional study of fish CD4 + T cells.…”

Section: Expression Of Transcription Factors and Cytokines In Cd4mentioning

confidence: 99%

“…Three CD4 + T cell subsets, CD4-1 single-positive T cells, CD4-2 single-positive T cells and CD4-1/CD4-2 doublepositive T cells, were found in different proportions in flounder peripheral blood, spleen and head kidney, and CD4-1 + /CD4-2 + T cells were the majority of CD4 + T cells 18 . In addition, dynamic changes in the percentages of CD4 + cells were detected as indicators of fish health and vaccine evaluation [19][20][21][22][23][24][25] . In this paper, to further explore the function of CD4 + Th cells, monoclonal antibodies against the flounder CD4-1 and CD4-2 molecules were developed.…”

mentioning

confidence: 99%

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Xing

Tian

Tang

et al. 2020

Sci Rep

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CD4 + T lymphocytes play crucial roles in the adaptive immune system. CD4, as the most effective marker to delineate the T-helper subsets, was identified in many fish species. Two CD4 homologs, CD4-1 and CD4-2, have been reported in flounder (Paralichthys olivaceus). In this study, monoclonal antibodies (mAbs) against CD4-1 and CD4-2 of flounder were produced, CD4 + T lymphocytes were isolated and identified, and the variations in CD4 + and CD8 + t lymphocytes and igM + B lymphocytes after Poly I:C, PMA or β-glucan stimulation were investigated. Then, the expression of transcription factors and cytokines in sorted CD4 + T lymphocytes was analyzed. The results showed that the mAbs were specific to flounder CD4-1 + and CD4-2 + T cells. CD4-1 + and CD4-2 + cells responded to all three stimulants, while CD8 + T lymphocytes only give a strong response to Poly I:C, and the percentages of igM + B lymphocytes showed a tendency to increase. After stimulation, the expression of transcription factors and cytokines of Th1, Th2 and Th17 cells varied in CD4 + T cells. These results will provide crucial foundations for the differentiation and function of teleost CD4 + t lymphocytes. CD4 + T cells, also known as T helper (Th) cells, play essential roles in the function of the immune system 1,2. They display extensive functional and phenotypic diversity in response to pathogens and promote cancer surveillance and tolerance to "self " antigens and environmental allergens 2. These functions are performed through specific antigen (Ag) recognition by CD4 + T cells and subsequent secretion of effector and regulatory cytokines 3. Upon Ag encounter, the T cell co-receptor CD4 present on T helper cells binds to the peptide/MHC (pMHC) class II molecules on antigen-presenting cells and transduces activation signals 4,5. Activated CD4 + Th cells can further differentiate into a variety of effector Th cell subsets, such as Th1, Th2, Th17 cells and regulatory T (Treg) cells 6 , and Th1 and Th2 cells were discovered to be the dominant populations of Th cells 7. The differentiation of Th cells towards a Th1 profile occurs in the presence of IL-12 and IFN-γ and is controlled by the master transcription factor T-bet 6. These Th1 cells secrete effector cytokines such as IFN-γ, TNF-a and IL-2 that stimulate macrophages and cytotoxic T lymphocytes (CTLs), which play important roles in cellular immunity against intracellular pathogens 6. Th2 cells, which are promoted by IL-4 and induction of GATA-3, produce cytokines IL-4, IL-5 and IL-13 that stimulate B cells to secrete different antibody isotypes and thus control extracellular infections 6,8,9. Th17 cells produce cytokines, including IL-17, IL-22, and IL-26, and they express the master transcription factor RORγt. They have a role in host defense against extracellular bacteria, fungi, inflammation and autoimmune diseases 10. Treg cells, which are regulated through Foxp3, have an essential role in regulating Th1-, Th2-, and Th17-type responses and are responsible for peripheral tolerance 11. Addition...

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“…baumannii through importing Acinetobacter NlpA gene into pEGFP-C2 vector (Hashemzehi et al 2018). Also, a DNA vaccine has developed against Vibrio (V.) anguillarum through construction of OmpK gene as immunogenic protein of V. anguillarum in pcDNA3.1 vector that can induce humoral and cellular immune responses in immunized fish with vector expressing OmpK gene (Xu et al 2019).…”

Section: Dna Vaccinesmentioning

confidence: 99%

Current trends in targeted therapy for drug-resistant infections

Rahbarnia

Farajnia

Naghili

et al. 2019

Appl Microbiol Biotechnol

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Escalating antibiotic resistance is now a serious menace to global public health. It may be led to the emergence of "postantibiotic age" in which most of infections are untreatable. At present, there is an essential need to explore novel therapeutic strategies as a strong and sustainable pipeline to combat antibiotic-resistant infections. This review focuses on recent advances in this area including therapeutic antibodies, antimicrobial peptides, vaccines, gene therapy, genome editing, and phage therapy for tackling drug-resistant infections.

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Intramuscular administration of a DNA vaccine encoding OmpK antigen induces humoral and cellular immune responses in flounder (Paralichthys olivaceus) and improves protection against Vibrio anguillarum

Cited by 24 publications

References 49 publications

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Current trends in targeted therapy for drug-resistant infections

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