Intramolecular DNA Coiling Mediated by a Metallo-Supramolecular Cylinder

Abstract

Passt genau: Ein tetrakationischer supramolekularer Zylinder, [Fe2L3]4+ (L=C25H20N4), mit einer tripelhelicalen Struktur hat genau die richtige Größe, um in die große Furche der DNA zu passen, während er für die kleine Furche zu groß ist (siehe Bild); NMR‐spektroskopische Untersuchungen belegen dies. Lineardichroismus‐Untersuchungen zufolge führt bereits eine sehr geringe Beladung mit [Fe2L3]4+ zu einer starken Krümmung der DNA. Mit rasterkraftmikroskopischen Aufnahmen konnte nachgewiesen werden, dass die Beladung intramolekulare Windungen der DNA zur Folge hat.

DNA binding and bending by dinuclear complexes comprising ruthenium polypyridyl centres linked by a bis(pyridylimine) ligand

“…This parent compound is tetracationic and has demonstrated strong non-covalent binding to DNA with exciting possibilities for structural control of DNA. When added to genomic or other long DNA, it intramolecularly coils up DNA [30][31][32][33], and when added to short palindromic oligonucleotides with AT-rich central sequences [34] it recognises or creates a three-way junction from three DNA oligonucleotides. Because of these exciting and unprecedented DNA binding properties we were intrigued to explore the effects on DNA of compounds with related structures.…”

“…Complexes based on ruthenium are particularly attractive because of their photochemical properties and their high kinetic stability towards racemisation and ligand exchange. However, this stability towards ligand exchange reactions does complicate the synthesis: the ruthenium triple-stranded cylinder can be prepared [6] but not in a high yielding supramolecular self-assembly reaction [30,31,34,35,38] as used to make the di-iron cylinder. The focus herein was the design of alternate ruthenium(II) compounds of type illustrated in Fig.…”

Recognition of DNA bulges by dinuclear iron(II) metallosupramolecular helicates

“…1) has been described previously [19,25,41,42]. Enantiomerically pure material was obtained by use of a cellulose column (~20 lM; Sigma-Aldrich) and elution with aqueous NaCl (20 mM) [43].…”

Investigation of the biological and anti-cancer properties of ellagic acid-encapsulated nano-sized metalla-cages

“…[10][11][12][13][14][15][16][17][18][19] Three-dimensional cages, engineered via coordinationdriven self-assembly paradigms, have distinct advantages over conventional covalentcontainer molecules, owing to the synthetic ease with which the coordination cages can be assembled, the availability of a large library of building blocks, and the ability to design selective guest encapsulations. 20,21 The strong and highly directional nature of metal-ligand interactions results in the formation of stable and rigid coordination…”