Intralesional Regulatory T-Cell Suppressive Function during Human Acute and Chronic Cutaneous Leishmaniasis Due to
            <i>Leishmania guyanensis</i>

Bourreau, Eric; Ronet, Catherine; Darcissac, Edith; Lise, Marie-Claude; Marie, D. Sainte; Clity, Emmanuel; Tacchini‐Cottier, Fabienne; Couppié, Pierre; Launois, Pascal

doi:10.1128/iai.01398-08

Cited by 70 publications

(60 citation statements)

References 32 publications

(37 reference statements)

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“…17 They were nevertheless diminished in peripheral blood compared with healthy subjects. 17 Other works also did not find increased numbers of Tregs in peripheral blood of chronic cutaneous leishmaniasis, although these cells overexpressed the inhibitory molecule CTLA-4. 10 They, however, accumulated in skin lesions and showed immunosuppressive activity.…”

Section: Discussionmentioning

confidence: 93%

“…Expression of both TGF-β and IL-10 was reported to be augmented in tuberculosis patients, but this expression was associated with other cells than Tregs. 9 In cutaneous leishmaniasis, a potential role was attributed to IL-10, with levels that were correlated with the parasite burden, 17 whereas others showed increased release of both IL-10 and TGF-β by Tregs derived from skin lesions as well as increased CTLA-4 expression by these cells. 10 Increased IL-10 expression but not CTLA-4 expression was associated with increased parasite burden in post-kala-azar dermal leishmaniasis.…”

Section: Discussionmentioning

confidence: 99%

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Increased Expression of Regulatory T Cells and Down-Regulatory Molecules in Lepromatous Leprosy

Palermo¹,

Pagliari²,

Trindade³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. T regulatory cells (Tregs) play an important role in the mechanism of host's failure to control pathogen dissemination in severe forms of different chronic granulomatous diseases, but their role in leprosy has not yet been elucidated; 28 newly diagnosed patients (16 patients with lepromatous leprosy and 12 patients with tuberculoid leprosy) and 6 healthy Mycobacterium leprae-exposed individuals (contacts) were studied. Tregs were quantified by flow cytometry (CD4+ CD25+ Foxp3+) in peripheral blood mononuclear cells stimulated in vitro with a M. leprae antigenic preparation and phytohemagglutinin as well as in skin lesions by immunohistochemistry. The lymphoproliferative (LPR), interleukin-10 (IL-10), and interferon-γ (IFN-γ) responses of the in vitro-stimulated peripheral blood mononuclear cells and the in situ expression of IL-10, transforming growth factor-β (TGF-β), and cytotoxic T-lymphocyte antigen 4 (CTLA-4) were also determined. We show that M. leprae antigens induced significantly lower LPR but significantly higher Treg numbers in lepromatous than tuberculoid patients and contacts. Mitogen-induced LPR and Treg frequencies were not significantly different among the three groups. Tregs were also more frequent in situ in lepromatous patients, and this finding was paralleled by increased expression of the antiinflammatory molecules IL-10 and CTLA-4 but not TGF-β. In lepromatous patients, Tregs were intermingled with vacuolized hystiocyte infiltrates all over the lesion, whereas in tuberculoid patients, Tregs were rare. Our results suggest that Tregs are present in increased numbers, and they may have a pathogenic role in leprosy patients harboring uncontrolled bacillary multiplication but not in those individuals capable of limiting M. leprae growth.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Increased Expression of Regulatory T Cells and Down-Regulatory Molecules in Lepromatous Leprosy

Palermo¹,

Pagliari²,

Trindade³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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“…7,20 Furthermore, the causative agent of CCL may affect the treatment. Thus, we took various clinical samples from skin lesions of suspected cases with CCL and cross-compared them using various laboratory methods.…”

Section: Laboratory Diagnosis In Chronic Cutaneous Leishmaniasismentioning

confidence: 99%

Comparison of Clinical Samples and Methods in Chronic Cutaneous Leishmaniasis

Eroglu¹,

Uzun²,

Koltas³

2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. This study aimed at finding out the most effective clinical samples and methods in chronic cutaneous leishmaniasis (CCL). Smear, aspiration fluid, and filter paper samples were taken from 104 skin lesions of suspected cases with CCL, and they were compared using microscopic examination, culture, and molecular methods. We characterized four different forms of CCL and identified the causative agents in CCL forms using high-resolution melting curve real-time polymerase chain reaction assay. We observed that smear was detected to be the most sensitive (63.5%) among clinical samples, and real-time polymerase chain reaction method was the most sensitive (96.8%) among the methods used in diagnosis of CCL. We identified 68.8% Leishmania tropica and 31.2% L. infantum in papular lesions, 69.2% L. infantum and 30.8% L. tropica in nodular lesions, 57.9% L. tropica and 42.1% L. major in ulcerating plaque lesions, and 55.5% L. tropica and 44.5% L. major in noduloulcerative lesions in CCL patients.

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“…The discovery of regulatory T cells (114) has shifted the focus of this research area toward CD4 T cells, and CD4 T cells are a dominant source of IL-10 in several different experimental infections with intracellular pathogens. IL-10-producing FoxP3 ϩ regulatory CD4 T cells have been detected in parasite infections, including those caused by T. cruzi (2), L. major (5), L. guyanensis (10), T. gondii (95), and P. yoelii (19), although the exact mechanism of their action has not been fully elucidated. For L. major, IL-10-producing CD4 ϩ CD25 ϩ FoxP3 ϩ T cells have been shown to inhibit the generation of memory recall immunity upon challenge with heat-killed parasites (93,94), indicating that IL-10-producing CD4 T cells can have a detrimental impact on immune responses, at least for the functional capacity of memory recall responses.…”

Section: Cellular Sources Of Il-10mentioning

confidence: 99%

Interleukin-10 and Immunity against Prokaryotic and Eukaryotic Intracellular Pathogens

2011

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2The generation of an effective immune response against an infection while also limiting tissue damage requires a delicate balance between pro-and anti-inflammatory responses. Interleukin-10 (IL-10) has potent immunosuppressive effects and is essential for regulation of immune responses. However, the immunosuppressive properties of IL-10 can also be exploited by pathogens to facilitate their own survival. In this minireview, we discuss the role of IL-10 in modulating intracellular bacterial, fungal, and parasitic infections. Using information from several different infection models, we bring together and highlight some common pathways for IL-10 regulation and function that cannot be fully appreciated by studies of a single pathogen.

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Intralesional Regulatory T-Cell Suppressive Function during Human Acute and Chronic Cutaneous Leishmaniasis Due to Leishmania guyanensis

Abstract: The levels of regulatory T cells (Treg cells)

Cited by 70 publications

References 32 publications

Increased Expression of Regulatory T Cells and Down-Regulatory Molecules in Lepromatous Leprosy

Increased Expression of Regulatory T Cells and Down-Regulatory Molecules in Lepromatous Leprosy

Comparison of Clinical Samples and Methods in Chronic Cutaneous Leishmaniasis

Interleukin-10 and Immunity against Prokaryotic and Eukaryotic Intracellular Pathogens

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