Intragastric and Intranasal Administration ofLactobacillus paracaseiNCC2461 Modulates Allergic Airway Inflammation in Mice

Abstract

Introduction. Preclinical and clinical evidences for a role of oral probiotics in the management of allergic diseases are emerging. Aim. We aimed at testing the immunomodulatory effects of intranasal versus intragastric administration of Lactobacillus paracasei NCC2461 in a mouse model of allergic airway inflammation and the specificity of different probiotics by comparing L. paracasei NCC2461 to Lactobacillus plantarum NCC1107. Methods. L. paracasei NCC2461 or L. plantarum NCC1107 strains were administered either intragastrically (NCC2461) or intranasally (NCC2461 or NCC1107) to OVA-sensitized mice challenged with OVA aerosols. Inflammatory cell recruitment into BALF, eotaxin and IL-5 production in the lungs were measured. Results. Intranasal L. paracasei NCC2461 efficiently protected sensitized mice upon exposure to OVA aerosols in a dose-dependent manner as compared to control mice. Inflammatory cell number, eotaxin and IL-5 were significantly reduced in BALF. Intranasal supplementation of L. paracasei NCC2461 was more potent than intragastric application in limiting the allergic response and possibly linked to an increase in T regulatory cells in the lungs. Finally, intranasal L. plantarum NCC1107 reduced total and eosinophilic lung inflammation, but increased neutrophilia and macrophages infiltration. Conclusion. A concerted selection of intervention schedule, doses, and administration routes (intranasal versus intragastric) may markedly contribute to modulate airway inflammation in a probiotic strain-specific manner.

Building upon current knowledge and techniques of indoor microbiology to construct the next era of theory into microorganisms, health, and the built environment

“…Colonization of the gut by L. johnsonii creates favorable conditions for co-colonization of the beneficial bacteria Bacillus fragilis by providing an N-glucan biosynthesis pathway, something which is reduced in the gut of asthmatics [79]. Oral probiotics are often effective in managing respiratory illness symptoms [80], and a small but encouraging amount of evidence exists on aerosolized or inhaled probiotics to similar effect [81,82]. Intranasal Lactobacillus paracasei reduced inflammation and immune system markers but increased neutropenia in the lungs of mice [81], and aerosolized Lactobacillus rhamnosus protected mice against transplanted lung tumors [82].…”

Role of Endogenous Microbiota, Probiotics and Their Biological Products in Human Health

“…, Enterococcus faecium JWS 833 and Faecalibacterium prausnitzii [70,71,72] have been reported to maintain immune homeostasis through modulating inflammation in DSS-induced colitis [34], alcohol-induced inflammation [70,73], cytokine-mediated gastrointestinal diseases [74], tumor bearing, and chemotherapy-induced mucositis in vivo , as well as pathogen infections in vitro [75]. For example, the probiotic Lactobacillus plantarum NCC1107 has been reported to reduce lung inflammation in mice by decreasing inflammatory cell numbers, eotaxin and IL-5 [76]. Moreover, dietary delivery of the probiotic Lactobacillus casei improved the immune response of mice bearing invasive ductal carcinomas, through significantly increasing production of IL-12 and IFN-γ and increasing NK cell cytotoxicity in spleen cell cultures, and most importantly, reduced tumor growth rate [77].…”

Effects of Lactobacillus paracasei 01 fermented milk beverage on protection of intestinal epithelial cell in vitro

“…Among various probiotic strains, Lactobacillus paracasei has been paid more attention recently because of its multiple probiotic activities, especially its intestinal‐protecting ability . Lactobacillus paracasei strains B21060 and CNCM I‐4034, isolated from feces of infants, are able to modulate dendritic cell activation and further reduce pathogenic Salmonella ‐induced pro‐inflammatory cytokine and chemokine production through Toll‐like receptor (TLR) signaling pathways in vitro .…”