Intrafollicular transfer of fresh and vitrified immature bovine oocytes

Sprícigo, J. F. W.; Netto, S. B. Sena; Muterlle, C. V.; Rodrigues, S. de A. D.; Leme, L. O.; Guimarães, A. L. S.; Caixeta, Felippe Manoel Costa; Franco, M. M.; Pivato, I.; Dode, M. A. N.

doi:10.1016/j.theriogenology.2016.07.003

Cited by 18 publications

(18 citation statements)

References 40 publications

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“…In that study, in vivo oocyte maturation rates after injecting 3 to 9 cumulus oocyte complexes (COCs) into preovulatory (30-36 mm) follicles were similar to in vitro maturation. Recently, we and others (Kassens et al, 2015;Spricigo et al, 2016) have tested the feasibility of injecting COCs into dominant follicles and confirmed that this technology can also be applied in cattle. This model in cattle still needs further validation to ensure that one preovulatory follicle is able to maintain injected-oocytes viability.…”

Section: Oocyte and Meiotic Resumptionsupporting

confidence: 60%

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Methods to study ovarian function in monovulatory species using the cow as a model

Rovani¹,

Gasperin

Ferreira³

et al. 2017

Anim Reprod

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In this review, we discuss the utility of the cow as an in vivo model to study the regulation of ovarian functions in monovular species. It is increasingly becoming clear that besides endocrine control, locally produced factors play pivotal roles during dominant follicle selection, oocyte maturation, ovulation and luteolysis. Although in vitro culture systems have been used to study these processes, definitive understanding the interactions between endocrine and local factors requires appropriate in vivo models. Most of the experimental approaches to study ovarian functions in vivo in large animals are based on the use of ultrasonography and considerable progress in this field has been made during the last thirty years. It has been shown that cows are an excellent model to collect samples (e.g., follicular fluid, granulosa cells, oocytes) from live animals at specific stages of follicular development in order to study mechanisms of intrafollicular factors in a physiological endocrine environment. In addition to support fundamental studies, the cow model has contributed immensely to the refinement of assisted reproductive technologies, which are now widely used not only in farm animals but also in humans.

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Section: Oocyte and Meiotic Resumptionsupporting

confidence: 60%

“…This model in cattle still needs further validation to ensure that one preovulatory follicle is able to maintain injected-oocytes viability. Recovery rates and embryo development rates need to be improved to allow the adoption of intrafollicular oocyte transfer (IFOT) under field conditions (Spricigo et al, 2016).…”

Section: Oocyte and Meiotic Resumptionmentioning

confidence: 99%

Methods to study ovarian function in monovulatory species using the cow as a model

Rovani¹,

Gasperin

Ferreira³

et al. 2017

Anim Reprod

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“…This study demonstrated that even in vitro maturation can be bypassed by direct transfer of collection cumulus-oocytes complexes into the follicle (Hoelker et al 2017). Intrafollicular oocyte transfer using immature fresh or vitrified oocytes collected from living donors using transvaginal aspiration was reported (Sprícigo et al 2016). Although IFOT did not improve the developmental competence of vitrified oocytes, viable blastocysts and pregnancies were achieved after transfer of fresh immature oocytes.…”

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confidence: 84%

Intrafollicular oocyte transfer in cattle – a technical report

et al. 2020

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The aim of the study was to develop and evaluate the functionality of a new equipment for intrafollicular oocyte transfer (IFOT) in dairy cattle. The new system for IFOT is composed of the applicator, the aspirator, and the injector. After aspiration of oocytes, the IFOT set is inserted into the working tube of the ultrasound transducer holder, the content of the applicator can be injected into the preovulatory follicle via transvaginal ultrasonography by one operator. The function of instruments used for IFOT was firstly verified in laboratory conditions. Slaughterhouse oocytes filled into the instruments were injected into Petri dishes. The highest recovery rates in vitro (97.5%) were achieved when the applicator was stored with the needle in a downward position. Synchronized Holstein heifers were used for in vivo test. Intrafollicular injection of saline (n = 9) was performed to find whether ovulation is affected by the injection. Then IFOTs of phosphate buffered saline with 20 oocytes (n = 21) were performed into the preovulatory follicles followed by 7-day-old embryo collection. Total ovulation rates were 86.7% (26/30). Total recovery rates (oocytes + embryos) were 23.1%, embryo recovery rates were 10.1%. The new instrument allows for the loading of oocytes and easy transportation to recipients, and also allows IFOT to be performed by one person in field conditions. The method does, however, need further investigation.

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“…However, the reported techniques did not support field applications. In the same way, the intra-follicular transfer of oocytes showed interesting results, but requires specialist skill to transfer oocytes into the follicular antrum (SPRÍCIGO et al, 2016). Thus, our group has proven the feasibility of nonsurgical embryo transfer techniques in small ruminants (FONSECA et al, 2014) and, in this study, we used this new technique to perform the maturation of bovine oocytes in a goat's uterus, whilst aiming to establish an efficient and practical method of ex situ maturation for bovine oocytes.…”

Section: Introductionmentioning

confidence: 94%

Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?

Fonseca

Batista

Trevizan

et al. 2019

SCA

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We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO 2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat's uterus could present an alternative to nuclear maturation.

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Intrafollicular transfer of fresh and vitrified immature bovine oocytes

Cited by 18 publications

References 40 publications

Methods to study ovarian function in monovulatory species using the cow as a model

Methods to study ovarian function in monovulatory species using the cow as a model

Intrafollicular oocyte transfer in cattle – a technical report

Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?

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