Intracytoplasmic sperm injection using microsurgically retrieved epididymal and testicular sperm

Mansour, Ragaa; Aboulghar, Mohamed; Serour, Gamal I.; Fahmi, Ibrahim Mohamed; Ramzy, Abdel Maguid; Amin, Yehia

doi:10.1016/s0015-0282(16)58155-2

Cited by 61 publications

(29 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Importantly, IVF-ICSI using surgically retrieved spermatozoa is the only method available that allows men with non-obstructive azoospermia (NOA) to father their own genetic offspring [Devroey et al 1995;Silber et al 1996]. The spermatozoa recovery rate in TESE has been shown to range from 40-70% [Mansour et al 1996;Friedler et al 1997;Schlegel et al 1997;Rosenlund et al 1998], and acceptable fertilization and pregnancy rates using testicular spermatozoa from men with NOA have been reported De Croo et al 2000;Mercan et al 2000;Balaban et al 2001]. Among the subgroups of NOA, Giorgetti et al [2005] reported that the fertilization rate was significantly lower when the patient exhibited maturation arrest (MA) as opposed to hypospermatogenesis (HS).…”

Section: Introductionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were assessed. No statistically significant differences were observed in any of the other measured parameters between the three subgroups of NOA. No significant differences in laboratory outcomes were observed between spermatozoa from fresh and frozen testicular spermatozoa; however, statistically significant differences were observed in the pregnancy and implantation rates between groups (p50.05). The outcomes of using spermatozoa retrieved from fresh testicular tissue in each of the three subgroups were also compared; although clinical outcomes showed low results, no significant differences were observed between the three subgroups. Similarly, no significant differences were observed in spermatozoa retrieved from frozen testicular tissue. Once spermatozoa have been successfully obtained, acceptable laboratory outcomes can be achieved for NOA, whether or not the spermatozoa are cryopreserved. However, satisfactory clinical outcomes may be more difficult to achieve as the results showed in each group of fresh and frozen testicular spermatozoa. Therefore, achieving acceptable clinical pregnancy results and efficient cryopreservation of testicular spermatozoa should be considered in patients with NOA.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Numerous literature report ICSI with spermatozoa recovered from epididymis (Silber et al 1994;Tournaye et al 1994;Mansour et al 1996) or testis (Schyosman et al 1993;Silber et al 1995). The fertilization and pregnancy rates from obstructive azoospermic patients are comparable to those obtained from patients with ejaculated sperm (Aboughar et al 1997).…”

Section: Icsi Results From Surgical Retrieved Spermmentioning

confidence: 98%

Effectiveness of Assisted Reproduction Techniques as an Answer to Male Infertility

Chamayou¹,

Guglielmino²

2012

Male Infertility

View full text Add to dashboard Cite

“…Therefore, ICSI is the technique of choice in cases of obstructive azoospermia since it enables the best use of microsurgically retrieved spermatozoa from the epididymis or testis (Schoysman et al, 1993a,b;Silber et al, 1994Silber et al, , 1995aTournaye et al, 1994;Mansour et al, 1996a).…”

Section: Clinical Application Of Icsimentioning

confidence: 99%

“…High fertilization and pregnancy rates have been reported after the combination of MESA and ICSI (Silber et al, 1994;Tournaye et al, 1994;Hovatta et al, 1995;Mansour et al, 1996a). However, the technique is lengthy, requires general anaesthesia, special skills and equipment, and results in a certain amount of trauma and postoperative morbidity.…”

Section: Clinical Application Of Icsimentioning

confidence: 99%

Intracytoplasmic sperm injection: a state of the art technique

Mansour

1998

Human Reproduction Update

View full text Add to dashboard Cite

Intracytoplasmic sperm injection using microsurgically retrieved epididymal and testicular sperm

Cited by 61 publications

References 17 publications

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effectiveness of Assisted Reproduction Techniques as an Answer to Male Infertility

Intracytoplasmic sperm injection: a state of the art technique

Contact Info

Product

Resources

About