Intracytoplasmic sperm injection: a state of the art technique

Mansour, Ragaa

doi:10.1093/humupd/4.1.43

Cited by 33 publications

(14 citation statements)

References 125 publications

(127 reference statements)

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“…Elongating spermatids corresponding to stage Sb2 have a dark, condensed nucleus, which resembles the head of a spermatozoon. The cytoplasm is shed from around the nucleus at one side, giving the nucleus an eccentric location and leaving the elongating spermatid with a very characteristic shape, resembling an ice‐cream cone, as described by Mansour (1998) (Figure 2a, b). Elongated spermatids corresponding to stage Sc and Sd1 (Figure 2c, d) have a prominent oval nucleus protruding partially from the cytoplasm, which surrounds the early‐formed tail.…”

Section: Methodsmentioning

confidence: 81%

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Mansour

Fahmy

Taha

et al. 2003

Journal of Andrology

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Almost one-third of all patients with nonobstructive azoospermia undergoing testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) have cancelled cycles due to failure to find spermatozoa. For these patients, every attempt should be made to rescue the cycles by searching for spermatids. In this retrospective study, we report our experience in using elongating (stage Sb2) and elongated (stage Sc and Sd1) spermatids for ICSI. The study included 488 consecutive ICSI and TESE cycles performed for 452 patients with nonobstructive azoospermia. In 179 (36.7%) cycles, neither spermatozoa nor mature spermatids (stage Sd2) suitable for injection were found. After an extensive search only Sb2, Sc, and Sd1 spermatids were found in 22 of these 179 cycles (12.3%). These spermatids were used for injection of retrieved oocytes. The fertilization rate was 33.2%, and 19 patients (86.4%) reached the embryo transfer stage. In 6 cycles a chemical pregnancy occurred, and 3 clinical pregnancies were established, resulting in the delivery of 3 healthy boys with normal karyotypes. When normal living spermatozoa or mature spermatids (stage Sd2) cannot be found during TESE, late spermatids (stage Sb2, Sc, and Sd1) can be used successfully and result in the delivery of healthy offspring.

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Section: Methodsmentioning

confidence: 81%

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Mansour

Fahmy

Taha

et al. 2003

Journal of Andrology

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“…Intracytoplasmic sperm injection (ICSI) procedure involving injection of a single sperm directly into a human egg under a microscope, is predominantly used for the treatment of male factor infertility 1 . The continuous improvements in controlled ovarian hyperstimulation (COHS), follicular monitoring, identification of top-quality embryos and embryo transfer procedures result in a remarkable rise in the pregnancy rate following cleavage embryo or blastocyst transfer in subjects undergoing ICSI; however, there are still 40 to 50% patients that fail in pregnancy 2 .…”

Section: Introductionmentioning

confidence: 99%

Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes

Lin

Jing

Zhu

et al. 2017

Sci Rep

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Previous studies have shown that GOLPH3 mediates cell growth, proliferation and differentiation and inhibits cell apoptosis; however, the role of GOLPH3 in cumulus granulosa cells and the value of GOLPH3 in predicting ICSI pregnancy outcomes remain unknown until now. Our findings showed higher positive expression rate, score of staining intensity, and immunohistochemical score of GOLPH3 in the cumulus granulosa cells of the pregnant women relative to non-pregnant women, and a higher apoptotic rate of cumulus granulosa cells was detected in non-pregnant women than in pregnant women. Pearson correlation analyses revealed that pregnancy correlated negatively with GOLPH3 expression and apoptosis of cumulus granulosa cells, and positively with the number of follicles punctured, number of grade III oocytes, number of eggs retrieved for ICSI, number of zygotes, number of cleavage-stage embryos, number of top-quality embryos, number of blastocysts, number of top-quality blastocysts, and number of frozen embryos. GOLPH3 may be involved in the apoptosis of cumulus granulosa cells, which may correlate with oocyte maturation and egg development. GOLPH3 expression in cumulus granulosa cells may facilitate the selection of top-quality eggs and embryos, the prediction of the clinical pregnancy outcomes of ICSI, and the increase of the pregnancy rate.

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“…Although the efficiency of ICSI has been clearly demonstrated [2,4,5], this procedure is typically associated with the degeneration of a significant percentage of treated oocytes [2,3,6,7]. The main cause of this degeneration remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

Khalilian¹,

Valojerdi²,

Navidbakhsh³

et al. 2013

ABB

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The precise determination of zona pellucida (ZP) hardness is largely unknown due to the lack of appropriate measuring and modelling methods. In this study, we have used experimental and theoretical models to describe the mechanical behavior of a single oocyte cell to improve the assisted reproductive technology (ART) outcomes by assessing oocyte/embryo quality. This paper presents the development of: i) a microinjection model to estimate the force of ZP penetration, ii) a micropipette aspiration model to determine the corresponding hardness, and iii) an experimental procedure to generate the required data for these two models. Our results show that the estimated penetration force provides a performance target for the penetration process during intracytoplasmic sperm injection (ICSI), while the estimated corresponding hardness serves as an indicator of the extent of deformation sustained by the oocyte prior to penetration. Evaluation of these results shows that a routine assessment of ZP hardness under microinjection would allow for the identification of certain oocyte pools for which further manipulation is recommended in order to improve injection, hatching and finally ART outcomes.

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Intracytoplasmic sperm injection: a state of the art technique

Cited by 33 publications

References 125 publications

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring

Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes

Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

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