Intracytoplasmic sperm injection: state of the art in humans

Palermo, Gianpiero D.; O’Neill, C.; Chow, Stephen; Cheung, Stephanie; Parrella, Alessandra; Rosenwaks, Zev

doi:10.1530/rep-17-0374

Cited by 95 publications

(67 citation statements)

References 206 publications

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“…With the development of in vitro fertilization (IVF) technologyespecially the use of intracytoplasmic sperm injection (ICSI)-several conditions (such as defective sperm-oocyte recognition) can be overcome. 1 Despite ICSI bypassing the zona pellucida and oolemma barriers, the incidence of total fertilization failure (TFF) after ICSI is reported to be 1% to 3%, 2,3 and defective oocyte activation is proposed to be a major cause of failed fertilization. [4][5][6] Oocyte activation is triggered by sperm-borne oocyte activation factor (SOAF) 7 ; and biochemical and clinical evidence indicates that 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (PLCζ) is a key SOAF.…”

Section: Introductionmentioning

confidence: 99%

Novel homozygous variations in PLCZ1 lead to poor or failed fertilization characterized by abnormal localization patterns of PLCζ in sperm

Dai

Guo

et al. 2019

Clinical Genetics

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Total fertilization failure (TFF), which is the failure of fertilization in all oocytes, occurs in 1%-3% of intracytoplasmic sperm injection (ICSI) cycles. However, the sperm-related factors that cause fertilization failure in humans are still largely unknown. Here, we identified three novel homozygous variations in the PLCZ1 gene in a recessive inheritance pattern in three consanguineous families, which all located in a key catalytic domain, and predicted to modify its secondary structure and thus impair its hydrolytic activity. Moreover, immunofluorescent staining revealed that PLCζ in mutant sperm exhibited abnormal localization patterns. ICSI-AOA resulted in an increased rate of normal fertilization compared with previous ICSI cycles (75.0% vs 2.2%, P < .001). In summary, we identified three novel homozygous variations in PLCZ1 that led to poor or failed fertilization that could be overcame by ICSI-AOA. K E Y W O R D Sassisted oocyte activation, fertilization failure, Phophospholipase C zeta, variation

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Section: Introductionmentioning

confidence: 99%

Novel homozygous variations in PLCZ1 lead to poor or failed fertilization characterized by abnormal localization patterns of PLCζ in sperm

Dai

Guo

et al. 2019

Clinical Genetics

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“…Laboratory procedures related to ICSI (e.g., sperm extraction and selection methods, sample handling, egg retrieval and culture, etc.) may further contribute to the likelihood of epigenetic alterations (Esteves et al, 2018; Ghosh et al, 2017; Palermo et al, 2017). Environmental events occurring during lifetime of individuals are known to produce modifications in gene expression that affect neurodevelopment and psychological function across the lifespan (Grigorenko et al, 2016; Guan et al, 2015), and there is evidence that some of these modifications may be inherited by offspring (Babenko et al, 2015; Chen et al, 2016; Nestler, 2016; Jablonka & Raz, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The use of ICSI as the treatment of choice for various types of infertility has raised concerns regarding its overuse, especially in light of the possibility of adverse postnatal outcomes (Esteves et al, 2018). ICSI has been responsible for over two million births since its inception (Palermo et al, 2017). As the earliest ICSI babies are now reaching maturity, researchers have become increasingly concerned with examining ICSI outcomes in various domains.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Operant Learning and Memory in Mice Born through Intracytoplasmic Sperm Injection

Lewon

Wang

Peters

et al. 2020

Preprint

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21Study question: Are there differences in operant learning and memory between mice born 22 through intracytoplasmic sperm injection (ICSI) and naturally-conceived control (CTL) mice? 23 Summary answer: ICSI females exhibited deficits in acquisition learning relative to CTL 24 females, whereas ICSI males exhibited deficiency in discrimination learning and memory 25 relative to CTL males during initial assessments. ICSI and CTL groups exhibited equally poor 26 long-term retention of learned discrimination and memory performances at old age. 27What is known already: Some human outcome studies have suggested that ICSI might be 28 associated with an increased risk of certain cognitive disorders, but only one of two behavioral 29 studies with ICSI mouse models have reported differences between ICSI and CTL females. No 30 studies to date have investigated associative learning in ICSI mice. 31 Study design, size, duration: 36 ICSI mice (18 male, 18 female) and 37 CTL mice (19 male, 18 32 female) aged 3-6 months were compared in a series of operant learning procedures that assessed 33 acquisition of a new behavior, discrimination learning, and memory. 16 ICSI mice (9 male, 7 34 female) and 17 CTL mice (10 males, 7 females) received follow-up discrimination learning and 35 memory assessments at 12 months of age (six months after the end of initial training) to evaluate 36 retention and reacquisition of learned performances. 37 Participants/materials, setting, methods: Mice received daily operant learning sessions in 38 experimental chambers in which all stimulus events and the recording of responses were 39 automated. Food rewards were delivered for responding under different conditions of 40 reinforcement, which varied by procedure. Subjects received a successive series of sessions of 41 nose poke acquisition training, discrimination training, and the delayed non-matching-to-position 42 (DNTMP) memory procedure. Mixed repeated measures ANOVAs in which the between-43Operant Learning in ICSI Mice 3 subjects factor was group (ICSI vs. CTL) and the within-subjects factor was repeated exposures 44 to learning procedures (i.e., sessions) were used to analyze data. 45Main results and the role of chance: In comparisons between all mice (i.e., males and females 46 combined), CTL mice exhibited superior performance relative to ICSI in response acquisition (p 47 = 0.03), discrimination (p = 0.001), and memory (p = 0.007). Sex-specific comparisons between 48 the groups yielded evidence of sexual dimorphism. ICSI females exhibited a deficit in 49 acquisition learning relative to CTL females (p < 0.001) but there was not a significant difference 50 between CTL and ICSI males. In the discrimination and memory tasks, ICSI males exhibited 51 deficits relative to CTL males (p = 0.002 and p = 0.02, respectively) but the differences between 52 females in these tasks were not significant. There was no difference in discrimination or memory 53 retention/re-acquisition assessments conducted with mice at 12 months of age. ICSI males and 54 fema...

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“…In addition, studies of epigenetic errors in oocytes are potentially confounded by contamination by DNA from somatic cells derived from the cumulus which could mimic abnormal methylation. 38…”

Section: Oocyte Maturation Manipulation and Epigenetic Eventsmentioning

confidence: 99%

Oocyte and Embryo Manipulation and Epigenetics

2018

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Regulation of the epigenome is a mechanism by which the environment influences gene expression and consequently the health of the individual. The advent and refinement of novel assisted reproductive technology (ART) laboratory techniques, including vitrification, dynamic culture systems, oocyte in vitro maturation, laser-assisted hatching, intracytoplasmic sperm injection, and preimplantation genetic testing for aneuploidy have contributed to the success of ART. From fertilization through implantation, the epigenetic profile of the embryo changes dynamically. Concurrently with these changes, embryo development in vitro is dependent on laboratory intervention and manipulation to optimize outcomes. The impact of ART techniques on imprinting errors remains unclear, as the infertile population likely confers an independent risk factor for defects in expected epigenetic patterns. Alternations in epigenetic mechanisms may contribute to the incidence of aneuploidy as well as recurrent implantation failure of euploid embryos. Additional investigative efforts are needed to assess the contribution of oocyte and embryo manipulation on imprinting modifications in this vulnerable population. The development of diagnostic modalities involving the discovery of epigenetic alterations to improve in vitro fertilization outcomes is an exciting and promising area of future study.

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Intracytoplasmic sperm injection: state of the art in humans

Cited by 95 publications

References 206 publications

Novel homozygous variations in PLCZ1 lead to poor or failed fertilization characterized by abnormal localization patterns of PLCζ in sperm

Novel homozygous variations in PLCZ1 lead to poor or failed fertilization characterized by abnormal localization patterns of PLCζ in sperm

Assessment of Operant Learning and Memory in Mice Born through Intracytoplasmic Sperm Injection

Oocyte and Embryo Manipulation and Epigenetics

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