Intracytoplasmic sperm injection overcomes previous fertilization failure with conventional in vitro fertilization

Benadiva, Claudio; Nulsen, John; Siano, L.; Jennings, Jean; Givargis, Helen Bet; Maier, Donald

doi:10.1016/s0015-0282(99)00403-3

Cited by 40 publications

(24 citation statements)

References 14 publications

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“…However, the most popular ICSI application is to salvage prior fertilization failure (6,(13)(14)(15). Results of these studies are equivocal at times.…”

mentioning

confidence: 99%

Use and outcomes of intracytoplasmic sperm injection for non–male factor infertility

Kim

Bundorf²,

Behr³

et al. 2007

Fertility and Sterility

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“…However, the most popular ICSI application is to salvage prior fertilization failure (6,(13)(14)(15). Results of these studies are equivocal at times.…”

mentioning

confidence: 99%

Use and outcomes of intracytoplasmic sperm injection for non–male factor infertility

Kim

Bundorf²,

Behr³

et al. 2007

Fertility and Sterility

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“…Firstly, data from couples suffering from male-factor infertility clearly demonstrate benefits from the use of ICSI [4,5,24,39]. Secondly, many clinics that currently utilize ICSI at a high rate demonstrate excellent pregnancy results.…”

Section: Discussionmentioning

confidence: 99%

Sibling Oocytes Randomly Assigned to Either Conventional Fertilization or Intracytoplasmic Sperm Injection Demonstrate Equivalent Fertilization and Blastulation Rates

Tobler¹,

Brezina²

2014

J IVF Reprod Med Genet

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Background: To determine the difference in fertilization and blastulation rates between sibling oocytes randomly assigned to either Conventional Fertilization (CF) or Intracytoplasmic Sperm Injection (ICSI) in patient couples with normal semen analysis parameters. Methods:A retrospective review of embryologic and clinical outcomes from patients undergoing their first In Vitro Fertilization (IVF) cycle that randomly assigned one-half of sibling oocytes to either CF or ICSI. Randomization occurred prior to removal of the oocyte cumulus cells which allows identification of metaphase II oocytes eligible for ICSI. All male partners met normal semen analysis parameters and normal fertilization was anticipated for the IVF cycle. Each patient served as their own control.Results: A total of 682 oocytes were included and were assigned to either CF or ICSI. The CF had a significantly higher fertilization rate compared to ICSI (67.9% versus 60%) when immature (non-metaphase II) oocytes were included in the analysis; however this difference diminished when only those oocytes eligible for ICSI were included. There was no difference in the day-3 embryo progression, the blastulation rate, the mean number of oocytes fertilized and the mean number of blastocysts per IVF cycle. Additionally, there was no significant difference in the pregnancy related outcomes between the two groups.Conclusions: These studies demonstrates that in patient couples with normal semen analysis parameters, undergoing their first IVF cycle and who have anticipate normal fertilization, randomly assigning oocytes to CF or ICSI does not improve fertilization or blastulation. To confirm these findings, larger prospective studies are required.

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“…The fertilized oocytes were transferred to HTF with 20% SSS (Irvine Scientific, USA) and cultured for another 24-48 h before being transferred to the mother uterus. A failure in the fertilization process was considered when the number of fertilized oocytes in relation to the number of injected oocytes was less than 50% [22]. Oocytes that failed to fertilize were evaluated microscopically to determine whether the failure of fertilization was due to problems in the chromatin decompactation process.…”

Section: Procedures For Icsimentioning

confidence: 99%

Sperm chromatin stability and its relationship with fertilization rate after Intracytoplasmic Sperm Injection (ICSI) in an assisted reproduction program

García¹,

Noriega-Hoces²,

Gonzales

2007

J Assist Reprod Genet

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Objective Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. Design Prospective study. Setting Institute of Gynecology and Reproduction. Patients Thirty-three women with their respective partners (12 couples in the study group and 21 couples in the control group) participating in a program of assisted reproduction. The study group was defined as men with >30% of nondecondensed spermatozoa (high sperm chromatin stability). Interventions A part of each seminal sample was used to evaluate sperm chromatin stability under SDS and EDTA treatment and the second aliquot was used for the ICSI procedure. Fertilization was evaluated 16-18 h post sperm injection at a pronuclear stage. The fertilized oocytes were further cultured for 24-48 h before transfer to the patient. Main outcome measures Fertilization rate.Results Thirty-five oocytes (35.7%) in the group of study and 109 oocytes (78.9%) in the control group showed two pronuclei (P<0.001). The coefficient of determination between the SDS+EDTA (Grade 2) and rate of fertilization was r 2 =0.85 (P<0.001) and the coefficient of regression was 1.72±0.19 (β±ES) (P<0.001). Conclusions High sperm chromatin stability is a factor which reduces the rate of fertilization after ICSI procedure.

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Intracytoplasmic sperm injection overcomes previous fertilization failure with conventional in vitro fertilization

Cited by 40 publications

References 14 publications

Use and outcomes of intracytoplasmic sperm injection for non–male factor infertility

Use and outcomes of intracytoplasmic sperm injection for non–male factor infertility

Sibling Oocytes Randomly Assigned to Either Conventional Fertilization or Intracytoplasmic Sperm Injection Demonstrate Equivalent Fertilization and Blastulation Rates

Sperm chromatin stability and its relationship with fertilization rate after Intracytoplasmic Sperm Injection (ICSI) in an assisted reproduction program

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