Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility

Palermo, Gianpiero D.; Cohen, Jacques; Alikani, Mina; Adler, Alexandra; Rosenwaks, Zev

doi:10.1016/s0015-0282(16)57603-1

Cited by 330 publications

(154 citation statements)

References 24 publications

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“…Infertility in such individuals can be circumvented by use of ART. Both IVF and ICSI have been successfully used in couples where the male partner has asthenozoospermia and not unexpectedly the success rates of ICSI are higher over IVF [10,31,46]. However, retrospective analysis of the ART outcomes have demonstrated that the fertilization rates, embryo qualities and pregnancy rates are poor when spermatozoa with low motility have been used for fertilization as compared to those with normal motility [14,17,28].…”

Section: Discussionmentioning

confidence: 99%

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Bhilawadikar

Zaveri²,

Mukadam³

et al. 2013

J Assist Reprod Genet

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Purpose To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate. Methods Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA. Results Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men.The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate. Conclusion Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI.

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Section: Discussionmentioning

confidence: 99%

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Bhilawadikar

Zaveri²,

Mukadam³

et al. 2013

J Assist Reprod Genet

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“…For fresh ET the pregnancy rate for ICSI cycles seems to be comparable to that of conventional IVF attempts (5,6). This rate is somewhat lower for frozen-thawed ET cycles of both ICSI and conventional IVF and is reported to range between 12.9 and 21% (7)(8)(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 92%

Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization

Simon

Holzer

Hurwitz

et al. 1998

J Assist Reprod Genet

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“…With this procedure, patients whose semen samples were insufficient for intrauterine insemination or in vitro fertilisation techniques could achieve pregnancy through the use of micromanipulation techniques [2]. Although oocyte quality has been suggested to be a strong determinant for success during assisted reproduction [3][4][5], it is generally thought that the quality of ejaculated spermatozoa has little effect on outcome [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

Wilding¹,

Coppola²,

Matteo

et al. 2010

J Assist Reprod Genet

118

100

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Purpose We used computer assisted sperm selection (MSOME) during cycles of intracytoplasmic sperm injection to test whether this technique improves results over traditional ICSI protocols. We also used the TUNEL assay to test whether MSOME could deselect physiologically abnormal spermatozoa. Methods Individual spermatozoa were examined with MSOME. Normal and abnormal spermatozoa were tested for the level of DNA fragmentation using TUNEL assay. In a prospective, randomized trial, patients were selected for standard ICSI, or IMSI techniques. We tested the two groups for biological and clinical parameters. Results 64.8% of spermatozoa, otherwise selectable for ICSI, were characterized by abnormalities after computer-assisted sperm analysis. These sperm were also characterized by an increase in the level of DNA fragmentation. We noted an increase in embryo quality, pregnancy and implantation rates after computerized sperm selection during ICSI procedures. Conclusions Computerised selection of spermatozoa during ICSI procedures deselects physiological abnormal spermatozoa and improves clinical results.

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Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility

Cited by 330 publications

References 24 publications

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

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