Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization

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“…Both Al-Hasani et al (20) and Hoover et al (21) have shown that ICSI-generated embryos frozen at the pronuclear stage have survival and pregnancy rates similar to those of conventionally generated embryos. A more recent study (22), using embryos frozen at the multicellular stage, has shown comparable survival rates for ICSI-and conventionally generated embryos, though in this study, the clinical pregnancy rate per transfer was significantly higher for IVF-generated embryos. Our own results indicate that cryopreservation is not detrimental to ICSI-generated embryos and that the survival and pregnancy rates of both pronuclear and multicellular frozen embryos are similar to those of corresponding embryos derived from conventional IVF.…”

Impact of fresh-cycle variables on the implantation potential of cryopreserved-thawed human embryos

“…There are data suggesting a difference between implantation rates following the transfer of frozen-thawed embryos generated from IVF or ICSI (13). This was mainly attributed to prefreezing embryo quality.…”

Analyzing Factors Affecting the Success Rate of Frozen–Thawed Embryos

“…Some retrospective studies were published comparing factors that may affect freezing-thawing IVF cycles including the influence of micromanipulation procedure on the success rate (10,11). However, the proportion of patients undergoing ICSI procedure has grown and patient's characteristics have changed.…”

“…Manual seeding was performed after 10 min at this temperature. Seeding was performed as previously described (10). After seeding, the tubes were held at −7.5 • C for another 15 min before the temperature was lowered to −30 • C at a rate of −0.3 • C/min.…”