The in vitro susceptibilities of 63 isolates of Stomatococcus mucilaginosus and of 188 isolates of Micrococcus spp. to 18 antimicrobial agents were determined by the agar dilution method. Many -lactams, imipenem, rifampin, and the glycopeptides were shown to be active in vitro against Stomatococcus and Micrococcus isolates, whereas the activities of antibiotics such as some aminoglycosides, erythromycin, and fosfomycin against an important number of these microorganisms are limited.The predominant class of microorganisms causing bacteremia, especially in neutropenic patients, has changed over the past 15 years from aerobic gram-negative bacilli to gram-positive cocci. Although Staphylococcus aureus remains the most commonly isolated agent, the list of gram-positive pathogens now includes a growing number of commensal bacteria: coagulase-negative staphylococci, viridans group streptococci, and, most recently, Stomatococcus mucilaginosus and Micrococcus spp.; both genera are members of the family Micrococcaceae (12,13,16). S. mucilaginosus is considered part of the normal flora of the human mouth and the upper respiratory tract (3). Though it appears to be an organism of low virulence, the recent increase in the number of reported cases of S. mucilaginosus bacteremia has established the pathogenic potential of this organism. The most frequent clinical presentations include septicemia, endocarditis, and catheter-related sepsis (2,8,9,12,13,15). Micrococcus spp. are found as normal inhabitants of human skin and mucous membranes and are usually disregarded as contaminants in clinical isolates (6). Nevertheless, strains identified as Micrococcus spp. have been involved as causative organisms in septicemia, endocarditis, central nervous system infection, peritonitis, and pneumonia (1,10,12,18).Previous studies have examined the susceptibilities either of single isolates or of a strain collection against selected compounds only (5,12,13,17). However, data on susceptibility patterns of a large number of isolates against a panel of antimicrobial agents are so far not available. Therefore, we tested the in vitro activities of a representative number of commonly used antibiotics against 63 isolates of S. mucilaginosus and against 188 isolates of micrococci.Bacterial strains. S. mucilaginosus was isolated from the mucous membrane of the cheek and gingiva; Micrococcus spp. were isolated additionally from the skin. A total of 36 isolates of S. mucilaginosus and 104 isolates of micrococci were obtained from swabs of 50 healthy persons; a total of 27 isolates of S. mucilaginosus and 84 isolates of micrococci were obtained from swabs of 50 neutropenic patients. At the time of specimen isolation, patients did not reveal any clinical signs of infections and had not been treated previously with antibiotics for at least 7 days.Isolates of S. mucilaginosus were identified on the basis of the following characteristics: the presence of gram-positive cocci in clusters; strong adherence of the usually mucoid, transparent, or whitish orga...