Intracerebroventricular injection of ghrelin and growth hormone releasing factor inhibits food intake in neonatal chicks

Furuse, Mitsuhiro; Tachibana, Tetsuya; Ohgushi, Atsushi; Ando, Ryuichi; Yoshimatsu, Takao; Denbow, D. Michael

doi:10.1016/s0304-3940(01)01621-4

Cited by 159 publications

(48 citation statements)

References 21 publications

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“…The stimulatory effects of GHRH and TRH on GH secretion in the chick are similarly partially mediated by actions within the CNS (Harvey 1990(Harvey , 1999. The possibility that ghrelin acts within the CNS is also supported by its ability to act at central sites to modulate food intake in the fowl (Furuse et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus)

Ahmed¹,

Harvey²

2002

Journal of Endocrinology

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Ghrelin, a recently discovered peptide in the mammalian hypothalamus and gastrointestinal tract is thought to be the endogenous ligand for the GH secretagogue (GHS) receptor and it stimulates GH release in rats and humans. The possibility that ghrelin is present in birds was therefore assessed, since a GHS receptor is present in the chicken pituitary gland.Although immunoreactive ghrelin is readily detectable in the rat stomach and ileum, ghrelin immunoreactivity could not be detected in the chicken proventriculus, stomach, ileum or colon, whereas somatostatin immunoreactivity, in contrast and as expected, was readily detectable in the chicken gastrointestinal tract. Ghrelin immunoreactivity was, however, present in the chicken hypothalamus, although not in the arcuate (infundibular) nucleus, as in rats. Discrete parvocellular cells and neuronal fibers with ghrelin immunoreactivity were present in the anterior medial hypothalamus. This immunoreactivity was specific and completely abolished following the preabsorption of the antibody with an excess of human ghrelin. Ghrelin immunoreactivity was also present in clusters of large ovoid magnocellular cells in the nucleus magnocellularis preopticus pars medialis, nucleus magnocellularis preopticus supraopticus and in the chiasmaopticus. Immunoreactivity for ghrelin was restricted to the cytoplasm of the perikarya and their axonal sprouts. Immunoreactivity for ghrelin was not seen in any other hypothalamic nuclei.In a preliminary experiment, circulating GH concentrations in conscious immature chicks were promptly increased following bolus i.v. administration of human ghrelin. The increase in GH concentration (approximately three times that in the controls) was comparable with that induced by the same dose (10 µg/kg) of human GHreleasing hormone, although less than that (approximately sixfold) induced by thyrotropin-releasing hormone.These results demonstrate the presence of a ghrelin-like protein in the chicken hypothalamus and suggest that it participates in the regulation of GH secretion in birds.

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Section: Discussionmentioning

confidence: 99%

Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus)

Ahmed¹,

Harvey²

2002

Journal of Endocrinology

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“…Previous studies have indicated that centrally injected rat ghrelin has a transient inhibitory effect on feed intake in neonatal chicks (Furuse et al, 2001;Saito et al, 2002). Shousha et al (2005) argued that a greater dose of ghrelin may have a down-regulating effect on growthhormone-releasing activity and is saturated following the administration of a high dose of ghrelin.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the hypothetical impact of ghrelin on bird food intake is deemed to be a research issue which has created a lot of controversial debates and arguments among interested scholars. Some studies (Furuse et al, 2001;Saito et al, 2002) have revealed that the central injection of ghrelin inhibits food intake in chickens. A dose-dependent stimulatory or inhibitory effect of ghrelin on food intake was investigated on Japanese quail (Shousha et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The effect of peripheral administration of ghrelin on the performance of growing geese

Shahryar

Lotfi

2015

Arch. Anim. Breed.

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Abstract. The purpose of the present study is to investigate the effect of intraperitoneal (IP) injection of ghrelin on goose performance. Hence, forty-eight 28-day-old geese were assigned into three treatments which lasted 40 days. The first intact group included no injection; that is, treatment 1 was characterized by G0; treatment 2, given to the second intact group, was characterized by G50, 50 ng kg −1 ghrelin body weight (BW); and treatment 3, given to the third intact group, was characterized by G100, 100 ng kg −1 ghrelin BW. Ghrelin was injected at the outset of the experimental rearing period (28-day-old birds). Blood samples were taken at two different times: (1) 12 h after the injection and (2) at the end of the rearing period. The effects of the injections were examined and evaluated during two rearing periods (28-48 days old and 48-68 days old). In the second treatment (G50), ghrelin injection caused an increase in the feed intake during the growing period but not the finishing period. Body weight gain and feed conversion ratio (FCR) of the growing period and finishing period did not change following any ghrelin treatments (P > 0.05). Injection of ghrelin at G100 increased breast muscle (pectoral) weight. The results of the present study indicate that ghrelin has a significant impact on feeding regulation and muscle growth at a certain period in geese. Nevertheless, it should be noted that ghrelin may have different effects on feeding of avian species.

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“…The ghrelin action is not equal in different animal species. Ghrelin stimulates feeding in rats; however, intracerebroventricular injection of ghrelin strongly suppressed feeding in neonatal chicks (5).…”

Section: Introductionmentioning

confidence: 93%

Immunohistochemistry grelinpositive cells in the pancreas islets of the rat

Val'kova¹,

Hamza²,

Ananiev³

et al. 2015

TJS

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After the discovery of the hormone ghrelin research on it is intensified in recent years. They are mainly clinical researches or studying of its physiological action. Fewer morphological experiments therefore histochemical expression of the cells that produce it, are relatively rare. The purpose of this study was by immunohistochemical methods to localize grelin-pozitive cells in the pancreas islets. We use Ghrelin (H-40) antibody (Santa Cruz-USA), and for the visualization of the results -detection system EnVisionTM FLEX Mini Kit, High Ph (DAKOk8024 HRP). Histomorphometric study performed by Michael Vinther Image Analyzer, Version 1.33 (Mee Soft, Germany).

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Intracerebroventricular injection of ghrelin and growth hormone releasing factor inhibits food intake in neonatal chicks

Cited by 159 publications

References 21 publications

Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus)

Ghrelin: a hypothalamic GH-releasing factor in domestic fowl (Gallus domesticus)

The effect of peripheral administration of ghrelin on the performance of growing geese

Immunohistochemistry grelinpositive cells in the pancreas islets of the rat

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