Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits

Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles

“…The prepared amyloid-beta plaque (222 μM) was stereotaxically administered (1 μL/min for 5 min) to bregma of the mice under anesthesia of isoflurane via intracerebroventricular (i.c.v.) injection using a Hamilton microsyringe (−0.2 mm anteroposterior (AP), −1 mm mediolateral (ML), and −2.4 mm dorsoventral (DV)) [ 46 ]. The stereotaxic surgical procedure was performed in a separate heated room whose heating system controls the body temperature of the mice (maintained at 36–37 °C).…”

Genetic interaction between Scn8a and potassium channel genes Kcna1 and Kcnq2

“…On postnatal day 2 (P2), mutant mice received 45 μg ASO in 2 μL by intracerebroventricular (ICV) injection into the left lateral ventricle. Adult mice were anesthetized with isoflurane and received 100 ug ASO in a 3 μL manual ICV injection into the left lateral ventricle without a guide cannula, as described 12 …”

Thermal cycling-hyperthermia ameliorates cognitive impairment of intracerebroventricular Aβ25-35-induced Alzheimer’s disease in C57BL/6 mice

“…Aβ 25-35 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) was dissolved in 0.9% sterile saline solution to a concentration of 1 mg/mL and then incubated at 37°C for 7 days for fibril aggregation. At the beginning of the experiment (Day 0), 10 μL Aβ solution was i.c.v.-injected into the mice’s brains of the Aβ, TC-HT, and HT groups according to the protocol described in the previous study [32]. 10 μL of sterile saline solution was i.c.v.-injected into the mice’s brains of the sham-operated control group…”

“…The study applied TC-HT to treat mice with AD, induced by intracerebroventricular (i.c.v.) injection of Aβ [25][26][27][28][29][30][31][32][33][34][35] , for comparison with the therapeutic effect of HT. In both cases, heat was applied to mouse brain specifically, to avoid potential side effect of whole-body HT.…”