Intracellular uptake of magnetite nanoparticles: A focus on physico-chemical characterization and interpretation of in vitro data

Némethová, Veronika; Buliaková, Barbora; Mazancová, Petra; Bábelová, Andrea; Selc, Michal; Moravčíková, Daniela; Kleščíková, Lucia; Ursı́nyová, Monika; Gábelová, Alena; Rázga, Filip

doi:10.1016/j.msec.2016.08.064

Cited by 16 publications

(6 citation statements)

References 38 publications

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“…Previously, we have shown that uptake of nanoparticles is a complex process and the amount of internalized nanoparticles depends on several factors such as number of treated cells, type of cell, type of particles, particle concentration, colloidal stability, etc. 64 Here we show clearly that a specic type of cell also derived from the same organ is important variable and that cell type specic nanoparticle uptake efficacy may be determining factor of cell sensitivity toward nanoparticle exposure. Previous ndings indicated that poor uptake of PEGylated nanoparticles by podocytes was most probably responsible for the weak effects observed.…”

Section: Discussionmentioning

confidence: 64%

Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

et al. 2020

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Section: Discussionmentioning

confidence: 64%

Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

et al. 2020

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“…As a dysopsonin protein, albumin promotes a prolonged blood circulation time through blocking the recognition by macrophages [ 42 ]. A comprehensive characterization of nanoparticles in biological fluids is, therefore, essential for the interpretation of their biological effects, including cellular uptake [ 43 ]. Nevertheless, PEG-SO-MNPs were more efficiently internalized into A549 cells than BSA-SO-MNPs.…”

Section: Discussionmentioning

confidence: 99%

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Svitkova¹,

Závišová²,

Némethová³

et al. 2021

Beilstein J. Nanotechnol.

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The efficient entry of nanotechnology-based pharmaceuticals into target cells is highly desired to reach high therapeutic efficiency while minimizing the side effects. Despite intensive research, the impact of the surface coating on the mechanism of nanoparticle uptake is not sufficiently understood yet. Herein, we present a mechanistic study of cellular internalization pathways of two magnetic iron oxide nanoparticles (MNPs) differing in surface chemistry into A549 cells. The MNP uptake was investigated in the presence of different inhibitors of endocytosis and monitored by spectroscopic and imaging techniques. The results revealed that the route of MNP entry into cells strongly depends on the surface chemistry of the MNPs. While serum bovine albumin-coated MNPs entered the cells via clathrin-mediated endocytosis (CME), caveolin-mediated endocytosis (CavME) or lipid rafts were preferentially involved in the internalization of polyethylene glycol-coated MNPs. Our data indicate that surface engineering can contribute to an enhanced delivery efficiency of nanoparticles.

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“…As iron was not detected in all doses and fractions analyzed, it is not possible infer a trend regarding the dependency of nanoparticles dose. Even when the doses appears to be a key factor on the up take efficiency other factors such as hydrodynamic size, stability on the physiological media, cell type, number of treated cells and the duration of the experiment must also be considered to assess a rigorous analysis [63]. The experiments carried out are the required preliminary stage in view of the future in vivo application in animal models to really validate the proposed magnetic formulation.…”

Section: Uptake Of Aa-mag4 Mnps By Hct116 Cellsmentioning

confidence: 99%

Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays

Schneider

Martín

Coral

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Magnetic iron oxide nanoparticles (MNPs) have been prepared and stabilized with three organic acids (tartaric, malic and ascorbic) in order to obtain biocompatible and water dispersible MNPs with potential to bind specifically to tumoral cancer cells. An in deep characterization was performed aiming to verify the presence and effect of the coating and stabilizer on MNPs surface. Besides the mechanisms followed by the different acids to bind MNPs were elucidated and used to justify the differences in the physicochemical properties of each formulation. Data related to characterization revealed that MNPs coated with ascorbic acid (MNPs-AA) resulted the most suitable in terms of their size, surface charge and stability along the time. Besides, ascorbic acid may be recognized by GLUTs receptors that are overexpressed in several kinds of tumoral cells. Therefore, MNPs-AA was selected to explore its performance in both MRI and in vitro assays using human colon cancer cells HCT 116. MRI experiments were performed in clinical equipment using a series of aqueous dispersions of MNPs-AA that were evaluated as T contrast agent. The T- weighted images obtained as well as the calculated r2, indicated that MNPs-AA could act as efficient T contrast agent for MRI. Regarding in vitro assays, MNPs-AA did not alter the cellular function neither exert cytotoxicity using the three explored doses. The internalization of the nanoparticles on the cellular structure was confirmed quanti and qualitatively using atomic absorption spectroscopy and Prussian blue techniques respectively. From these results, it emerges that ascorbic acid coated-magnetite nanoparticles may be used as alternative contrast agent to avoid or minimize some toxicological issues related to the widely used gadolinium.

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Intracellular uptake of magnetite nanoparticles: A focus on physico-chemical characterization and interpretation of in vitro data

Cited by 16 publications

References 38 publications

Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays

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