Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

Selc, Michal; Rázga, Filip; Némethová, Veronika; Mazancová, Petra; Ursı́nyová, Monika; Novotová, Marta; Kopecka, Kristina; Gábelová, Alena; Bábelová, Andrea

doi:10.1039/d0ra03133j

Cited by 3 publications

(1 citation statement)

References 73 publications

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“…Interestingly, the magnetic nanospheres (PEG-SO-MNPs coated with polylactic- co -glycolic acid, PLGA) were taken up by A549 cells to the same extent as BSA-SO-MNPs, even though their hydrodynamic particle size was more than five times larger [ 44 ]. A less efficient uptake of BSA-coated MNPs compared to PEG-coated MNPs has been observed also in primary murine podocytes [ 45 ]. Superparamagnetic iron oxide nanoparticles covered with BSA were shown to possess a lower affinity to the cellular membrane [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Svitkova¹,

Závišová²,

Némethová³

et al. 2021

Beilstein J. Nanotechnol.

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The efficient entry of nanotechnology-based pharmaceuticals into target cells is highly desired to reach high therapeutic efficiency while minimizing the side effects. Despite intensive research, the impact of the surface coating on the mechanism of nanoparticle uptake is not sufficiently understood yet. Herein, we present a mechanistic study of cellular internalization pathways of two magnetic iron oxide nanoparticles (MNPs) differing in surface chemistry into A549 cells. The MNP uptake was investigated in the presence of different inhibitors of endocytosis and monitored by spectroscopic and imaging techniques. The results revealed that the route of MNP entry into cells strongly depends on the surface chemistry of the MNPs. While serum bovine albumin-coated MNPs entered the cells via clathrin-mediated endocytosis (CME), caveolin-mediated endocytosis (CavME) or lipid rafts were preferentially involved in the internalization of polyethylene glycol-coated MNPs. Our data indicate that surface engineering can contribute to an enhanced delivery efficiency of nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Svitkova¹,

Závišová²,

Némethová³

et al. 2021

Beilstein J. Nanotechnol.

Self Cite

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show abstract

Zinc oxide nanoparticles prevent the onset of diabetic nephropathy by inhibiting multiple pathways associated with oxidative stress

Padhye-Pendse,

Umrani,

Paknikar

et al. 2024

Life Sciences

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Size and dose of nanoparticles modulate between toxic and medicinal effect on kidney

Nolte,

2024

OpenNano

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Surface coating determines the inflammatory potential of magnetite nanoparticles in murine renal podocytes and mesangial cells

Abstract: Primary renal podocytes are more susceptible to MNPs exposure than primary renal mesangial cells.

Cited by 3 publications

References 73 publications

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

Zinc oxide nanoparticles prevent the onset of diabetic nephropathy by inhibiting multiple pathways associated with oxidative stress

Size and dose of nanoparticles modulate between toxic and medicinal effect on kidney

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