Intracellular pH Sensors Based on Surface-Enhanced Raman Scattering

Talley, Chad E.; Jusinski, Leonard E.; Hollars, Christopher W.; Lane, Stephen M.; Huser, Thomas

doi:10.1021/ac049093j

Cited by 313 publications

(363 citation statements)

References 30 publications

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“…SERS probes can also be used as sensors for measuring other properties of cells, such as their pH, for example using 4-mercaptobenzoic acid as a reporter [298]. pH-sensitive AuNPs were also designed which could specifically target cancer cells, and aggregate inside to produce a diagnostic SERS reporter signal, while also allowing photothermal therapy from aggregate heating [299].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

show abstract

“…In this case, SERS has an advantage over fluorescence microscopy in that less harmful NIR excitation can be used. SERS can be applied to the study of physiological conditions such as pH [39], where silver nanoparticles were functionalised with 4-mercaptobenzoic acid, and the SERS spectrum is sensitive to pH changes in the range of 6-8. The principle is inherently simple and involves the use of SERS labels that change structure in response to environmental factors.…”

Section: Sers In and Of Cells And Bacteriamentioning

confidence: 99%

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Ryder

2005

Current Opinion in Chemical Biology

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Raman spectroscopy is rapidly finding favour for applications in the life science due to the ease with which it can be used to extract significant data from tissue and cells. However, the Raman effect is an inherently weak effect, which hinders the analysis of low concentration analytes. The Raman sensitivity can be improved via the Surface Enhanced Raman Scattering (SERS) effect. In SERS, Raman spectra are dramatically increased when a molecule is adsorbed onto nanoroughened noble metal surfaces such as silver and gold. The degree of enhancement enables single molecule detection, which offers the potential for the unambiguous identification of analytes at concentrations that are useful in both a forensic and a chemical biology context. Here we discuss some of the practical applications of SERS to both low-level narcotic detection, and how this can be applied to chemical biology.

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“…, whereas for those with dissociated carboxyl groups this spectral region is dominated by a band at 857 cm −1 [44,47,48]. In the SERS spectrum of MBA adsorbed on the Ag/TiO 2 NT/Ti (Fig.…”

Section: Sers Measurementsmentioning

confidence: 96%

TiO2 and Al2O3 nanoporous oxide layers decorated with silver nanoparticles—active substrates for SERS measurements

Pisarek

Hołdyński

Roguska

et al. 2014

J Solid State Electrochem

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Self-organized nanoporous oxide layers (TiO 2 , Al 2 O 3 ) exhibiting specific properties, obtained by anodic oxidation at a constant voltage in neutral electrolyte, may serve as attractive SERS substrates for investigating the interactions between an adsorbate and adsorbent, or as a stable platform for detecting various organic compounds. This paper presents the influence of the size of the nanotubes/nanopores and the structure of the porous oxide layers on the SERS enhancement factor, E F . We used pyridine and mercaptobenzoic acid as probe molecules, since they have a large cross-section for Raman scattering. To characterize the morphology and structure of the oxide layer substrates, before and after vacuum vapor deposition of silver nanoparticles, we applied scanning electron microscopy, X-ray diffraction and surface analytical techniques: AES, XPS and SERS. The results obtained show that for the same amount of Ag (0.02 mg/cm 2 ) the size of the nanopores significantly affects the E F , which reaches, at a properly chosen nanopore size, distinctly higher values than that characteristic of a standard silver surface roughened by electrochemical cycling, i.e. E F >10 6 . The new Ag/MeO x -NT composites layer, ensure a good reproducibility of the SERS measurements and exhibit stability over a limited period of time.

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Intracellular pH Sensors Based on Surface-Enhanced Raman Scattering

Cited by 313 publications

References 30 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

TiO2 and Al2O3 nanoporous oxide layers decorated with silver nanoparticles—active substrates for SERS measurements

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