Intracellular pH and CO2 combining curve of hypertrophic cardiac muscle in rats

Saborowski, F; Scholand, Ch.; Lang, D.; Albers, C.

doi:10.1016/0034-5687(73)90047-9

Cited by 11 publications

(2 citation statements)

References 6 publications

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“…Calculations of NAD+/NADH ratios from substrate couples require a knowledge of intracellular [H+] which we did not measure. An unchanged tissue lactate content after increased heart work is presumptive evidence against an altered tissue [H+I], whereas Saborowski et al (1973) used 5,5-di['4C]methyl-2,4-oxazolidinedione to show that increased mechanical load on the rat heart in situ only slightly changed the intracellular pH from 6.95 to 7.07. A larger change in the mitochondrial pH cannot be excluded.…”

Section: Methodsmentioning

confidence: 99%

Effects of increased mechanical work by isolated perfused rat heart during production or uptake of ketone bodies. Assessment of mitochondrial oxidized to reduced free nicotinamide-adenine dinucleotide ratios and oxaloacetate concentrations

Opie¹,

Owen²

1975

Biochemical Journal

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Metabolic effects of increased mechanical work were studied by comparing isolated pumping rat hearts perfused by the atrial-filling technique with aortic-perfused non-pumping hearts perfused by the technique of Langendorff. The initial medium usually contained glucose (11 mm) and palmitate (0.6 mm bound to 0.1 mm albumin). During increased heart work (comparing pumping with non-pumping hearts) the uptake of oxygen and glucose increased threefold, but that of free fatty acids was unchanged. Tissue contents of alpha-oxoglutarate, NH4+, malate, lactate, pyruvate and Pi rose with increased heart work, but contents of ATP, phosphocreatine and citrate fell. Ketone bodies were produced with a ratio of beta-hydroxybutyrate/acetoacetate of about 3:1 in both pumping and non-pumping hearts but with higher net production rates in non-pumping hearts. When ketone bodies were added in relatively high concentrations (total 4 mm) to a glucose (11 mm) medium the medium, ratios of beta-hydroxybutyrate/acetoacetate were not steady even after 60 min of perfusion. The validity of calculating mitochondrial free NAD+/NADH ratios from the tissue contents of the reactants of the glutamate dehydrogenase system or the beta-hydroxybutyrate dehydrogenase system is assessed. The activities of these enzymes are considerably less in the rat heart than in the rat liver, introducing reservations into the application to the heart of the principles used by Williamson et al. (1967) for calculation of mitochondrial free NAD+/NADH ratios of liver mitochondria...

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Section: Methodsmentioning

confidence: 99%

Effects of increased mechanical work by isolated perfused rat heart during production or uptake of ketone bodies. Assessment of mitochondrial oxidized to reduced free nicotinamide-adenine dinucleotide ratios and oxaloacetate concentrations

Opie¹,

Owen²

1975

Biochemical Journal

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“…This increase was in the same order of magnitude as in aortic stenosis 6 days after ligation of the aorta where the pooled data of Saborowski et al (4) and Herten and Albers (3) yielded a buffer value of 89 slyke. The buffer value of the intracellular space increased to 87 slyke (control 68 slyke).…”

Section: Resultsmentioning

confidence: 59%

The intracellular pH and the buffer capacity of the heart

Albers

1977

Basic Res Cardiol

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The intracellular buffering (delta HCO3-/delta pH) in rats is lowest in skeletal muscle, intermediate in the diaphragm and highest in cardiac muscle. In the hypertrophic heart due to aortic constriction or to the Goldblatt mechanism the buffer value increases markedly during the first weeks, but returns to normal or even subnormal values during stable hypertrophy. There is an electrochemical disequilibrium for hydrogen ions caused by an active transport of ions across the cell membrane, which is possibly correlated to the myocardial activity.

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The intracellular pH and the buffer capacity of the heart

Albers¹

1977

The Hypertrophied Heart

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Intracellular pH and CO2 combining curve of hypertrophic cardiac muscle in rats

Cited by 11 publications

References 6 publications

Effects of increased mechanical work by isolated perfused rat heart during production or uptake of ketone bodies. Assessment of mitochondrial oxidized to reduced free nicotinamide-adenine dinucleotide ratios and oxaloacetate concentrations

Effects of increased mechanical work by isolated perfused rat heart during production or uptake of ketone bodies. Assessment of mitochondrial oxidized to reduced free nicotinamide-adenine dinucleotide ratios and oxaloacetate concentrations

The intracellular pH and the buffer capacity of the heart

The intracellular pH and the buffer capacity of the heart

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