The intracellular pH and the buffer capacity of the heart

Simultaneous intracellular and extracelular pH measurement in the heart by 19F NMR of 6‐fluoropyridoxol

“…The intracellular pH determined for hearts in this study is similar to that reported by many previous studies, with a typical range of 7.02-7.20 for normally perfused hearts assessed using a variety of techniques (21,22,25,(35)(36)(37). Most studies have examined intracellular acidosis since this is found to accompany ischemia and leads to loss of contractile force.…”

“…The relative signal intensity of the 6-FPOL peaks could be controlled by adjusting the volume of perfusate bathing the heart. Spectra acquired in 2 min routinely provided signal-to-noise ratios of -8 and - 35 for intracellular and extracellular signals, respectively, with linewidths on the order of 160 Hz and 120 Hz after For the group of eight hearts, pH was found to be highly consistent. The downfield (intracellular) signal occurred in the range of -10.43 to -10.69 ppm, indicating pH = 7.00-7.18 with mean pHi = 7.11 t 0.07 (2SD, n = 8 hearts).…”