Intracellular pathways following uptake of bevacizumab in RPE cells

Reduction of GAPDH in lenses of Parkinson's disease patients: A possible new biomarker

“…In addition, Western blots were conducted as previously described to verify the results of LC‐MS/MS with anti‐GAPDH (Santa Cruz, Heidelberg, Germany) or αB‐crystallin (Santa Cruz) as primary antibodies and HRP‐tagged antirabbit (Cell Signaling Technology, Frankfurt, Germany) or antigoat (Cell Signaling Technology) secondary antibody. Molecular weight and densitometric volume of the bands were assessed as previously described . For densitometric evaluation of Western blots, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was normalized with αB‐crystallin.…”

An anti-TNF-α antibody mimetic to treat ocular inflammation

“…Antibody uptake into the retinal pigment epithelium (RPE)28 can occur via neonatal Fc-receptors expressed in RPE cells2930. Uptake of infliximab and FpF infliximab were assessed in vitro using a murine B6RPE-07 cell line31 in which cell cultures exhibit cobblestone-like morphology similar to primary mouse RPE cultures.…”

“…As a selective barrier, the RPE permits intraocular migration of leukocytes as well as a variety of physiological functions including the phagocytosis of shed photoreceptor outer segments and transport of molecules to and from the retina5152. Recent data shows that therapeutic anti-VEGF mAbs (bevacizumab and afilbercept) can be transported intracellularly across RPE cells, implicating these cells as important protagonists in the clearance of antibodies from the sub-retinal space into the choroid28. Uptake in the RPE is a general feature of Fc-fragment containing molecules such as IgG28, via Fc-receptors expressed in RPE cells2930.…”

“…Recent data shows that therapeutic anti-VEGF mAbs (bevacizumab and afilbercept) can be transported intracellularly across RPE cells, implicating these cells as important protagonists in the clearance of antibodies from the sub-retinal space into the choroid28. Uptake in the RPE is a general feature of Fc-fragment containing molecules such as IgG28, via Fc-receptors expressed in RPE cells2930. Using in vitro RPE cells, no sub-retinal uptake was observed for FpF infliximab .…”

Micro/Nanoparticle Delivery Systems for Ocular Diseases

“…collect large incoming particles and digest them with the assistance of lysosomes. Indigestible material then exits the cell through exocytosis 80 (Fig. 4A).…”