Intracellular macrophage uptake of rifampicin loaded mannosylated dendrimers

Kumar, Palanirajan Vijayaraj; Asthana, Abhay; Dutta, Tathagata; Jain, Narendra Kumar

doi:10.1080/10611860600825159

Cited by 192 publications

(77 citation statements)

References 32 publications

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“…We attribute the enhanced toxicity attenuation properties of the sialic acid containing polymers to the increased sialic acid valency in these materials. There are many examples of the role of valency in adhesion of ligands to multivalent materials [48][49][50][51][52][53]. More specific to Aβ, others have shown for biological membranes that Aβ binding affinity to sialic acid containing gangliosides increased in clustered or multivalent regions of the membrane compared to membrane regions where sialic acids or gangliosides were unclustered [25,30].…”

Section: Discussionmentioning

confidence: 99%

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

2008

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Abstractβ-Amyloid peptide (Aβ), the primary protein component in senile plaques associated with Alzheimer's disease (AD), has been implicated in neurotoxicity associated with AD. Previous studies have shown that the Aβ-neuronal membrane interaction plays a crucial role in Aβ toxicity. More specifically, it is thought that Aβ interacts with ganglioside rich and sialic acid rich regions of cell surfaces. In light of such evidence, we have hypothesized that the Aβ-membrane sialic acid interaction could be inhibited through use of a biomimic multivalent sialic acid compound that would compete with the cell surface for Aβ binding. To explore this hypothesis, we synthesized a series of photocrosslinked sialic acid containing oligosaccharides and tested their ability to bind Aβ and attenuate Aβ toxicity in cell culture assays. We show that a polymer prepared via the photocrosslinking of disialyllacto-N-tetraose (DSLNT) was able to attenuate Aβ toxicity at low micromolar concentrations without adversely affecting the cell viability. Polymers prepared from mono-sialyl-oligosaccharides were less effective at Aβ toxicity attenuation. These results demonstrate the feasibility of using photocrosslinked sialyl-oligosaccharides for prevention of Aβ toxicity in vitro and may provide insight into the design of new materials for use in attenuation of Aβ toxicity associated with AD.

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Section: Discussionmentioning

confidence: 99%

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

2008

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“…50% (free RIF) to 85% ( RIF dendrimers). The phagocytic uptake of RIF and RIF-loaded dendrimers in alveolar macrophages harvested from rat lungs showed a clear increase in the intracellular concentration of the antibiotic [101]. A more recent reports investigated 4G and 5G PEGylated-PPI dendrimers for sustain the delivery of RIF.…”

Section: Niosomesmentioning

confidence: 99%

Present status of nanoparticle research for treatment of Tuberculosis

2011

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-Nanotechnology has offered enormous improvement in field of therapeutics by means of designing of drug delivery systems and opened the possibility of controlling infections at the molecular level. Nanocarriers can cross biological barriers and are able to target cellular reservoirs of Mycobacterium tuberculosis (M. tuberculosis). Nanoparticle-based systems have significant potential for treatment and prevention of tuberculosis (TB). A variety of nanocarriers have been widely evaluated as potential drug delivery systems for various administration routes. Targeting the drugs to certain physiological sites such as the lymph nodes has emerged as a promising strategy in treating TB with improved drug bioavailability and reduction of the dosing frequency. Nanotechnology based rational targeting may improve therapeutic success by limiting adverse drug effects and requiring less frequent administration regimes, ultimately resulting in more patients compliance and thus attain higher adherence levels. The development of nanoparticle based aerosol vaccine is undergoing which could serve as new platform for immunization. Present article compiles the general physiological aspects of the infection along with new research uptades on nanocarriers used in prevention of tuberculosis. _______________________________________________________________________________________

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“…In our previous study, we explored concentrations as high as 6 per cent [25]. The RIF aqueous solubility was increased up to 5.4 times, this performance being better than that of cyclodextrins [28] and mannose-grafted poly(propyleneimine) dendrimers [29]. Independently of the copolymer molecular weight, the PEG/PCL molar ratio and its concentration, drug-free micelles display a relatively low physical stability [25,30].…”

Section: Introductionmentioning

confidence: 99%

Cryoprotection–lyophilization and physical stabilization of rifampicin-loaded flower-like polymeric micelles

Morettón

Chiappetta

Sosnik

2011

J. R. Soc. Interface.

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Rifampicin-loadedpoly(e-caprolactone)-b-poly(ethylene glycol) -poly(e-caprolactone) flower-like polymeric micelles display low aqueous physical stability over time and undergo substantial secondary aggregation. To improve their physical stability, the lyoprotectionlyophilization process was thoroughly characterized. The preliminary cryoprotectant performance of mono-and disaccharides (e.g. maltose, glucose), hydroxypropyl-bcyclodextrin (HPbCD) and poly(ethylene glycol) (PEG) of different molecular weights was assessed in freeze -thawing assays at 2208C, 2808C and 21968C. The size and size distribution of the micelles at the different stages were measured by dynamic light scattering (DLS). A cryoprotectant factor ( f c ) was determined by taking the ratio between the size immediately after the addition of the cryoprotectant and the size after the preliminary freeze -thawing assay. The benefit of a synergistic cryoprotection by means of saccharide/ PEG mixtures was also assessed. Glucose (1 : 20), maltose (1 : 20), HPbCD (1 : 5) and glucose or maltose mixtures with PEG3350 (1 : 20) (copolymer:cryoprotectant weight ratio) were the most effective systems to protect 1 per cent micellar systems. Conversely, only HPbCD (1 : 5) cryoprotected more concentrated drug-loaded micelles (4% and 6%). Then, those micelle/ cryoprotectant systems that displayed f c values smaller than 2 were freeze-dried. The morphology of freeze-dried powders was characterized by scanning electron microscopy and atomic force microscopy and the residual water content analysed by the Karl Fisher method. The HPbCD-added lyophilisates were brittle porous cakes (residual water was between 0.8% and 3%), easily redispersable in water to form transparent systems with a minimal increase in the micellar size, as determined by DLS.

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Intracellular macrophage uptake of rifampicin loaded mannosylated dendrimers

Cited by 192 publications

References 32 publications

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

Present status of nanoparticle research for treatment of Tuberculosis

Cryoprotection–lyophilization and physical stabilization of rifampicin-loaded flower-like polymeric micelles

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