Intracellular ion levels in erythrocytes and hepatocytes isolated from three teleost species

Furimsky, Marosh; Perry, Steve F.; Moon, Tae-Chul

doi:10.1111/j.1095-8649.1999.tb00741.x

Cited by 3 publications

(2 citation statements)

References 30 publications

(24 reference statements)

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“…Gill cells are exposed to different ion gradients across their apical and basolateral surfaces as a result of their exposure to water and blood, respectively, requiring different membrane transport mechanisms for intracellular and blood ionic balance. Thus, when sodium concentrations are increased, potassium is generally decreased in fish plasma or serum (FURIMSKY et al, 1999). The maintenance of a consistent internal environment is essential for normal cells to function in multicellular organisms.…”

Section: Introductionmentioning

confidence: 99%

PARÂMETROS BIOQUÍMICOS DE Piaractus mesopotamicus E Colossoma macropomum (CHARACIDAE) E HÍBRIDO TAMBACU (P. mesopotamicus x C. macropomum)

Tavares‐Dias¹,

Moraes²

2010

Ci. Anim. Bras.

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A study for investigating the values of plasma glucose, serum total protein and serum ions (calcium, potassium, magnesium, sodium and chloride) was carried out in young Colossomoma macropomum Cuvier, 1818, Piaractus mesopotamicus Holmberg, 1887 and the hybrid tambacu (P. mesopotamicus x C. macropomum), kept in intensive system. Glucose concentrations were higher in tambacu than in P. mesopotamicus and C. macropomum. Total protein levels were higher in P. mesopotamicus than both C. macropomum and tambacu. C. macropomum presented higher concentrations of sodium and chloride, while P. mesopotamicus had higher concentrations of potassium and magnesium. However, levels of calcium were similar for the species studied. The hybrid tambacu showed the smallest levels of total protein, and intermediate levels of sodium, potassium, magnesium and chloride, compared to both P. mesopotamicus and C. macropomum. Baseline values for healthy fish in aquaculture were established, and they may be used for further comparisons in studies with wild populations of P. mesopotamicus and C. macropomum.

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Section: Introductionmentioning

confidence: 99%

PARÂMETROS BIOQUÍMICOS DE Piaractus mesopotamicus E Colossoma macropomum (CHARACIDAE) E HÍBRIDO TAMBACU (P. mesopotamicus x C. macropomum)

Tavares‐Dias¹,

Moraes²

2010

Ci. Anim. Bras.

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“…Peixes em contato com água natural de rios e riachos estão expostos a variações de gradientes iônicos através das células da brânquia, requerendo diferentes tipos de transporte iônico. Durante essas trocas o aumento na concentração sérica de sódio, ocasiona a redução na concentração sérica de potássio [9]. Embora não sendo estatisticamente significativa a concentração de potássio do presente estudo foi menor na estação do inverno.…”

Section: Discussionunclassified

Serum Biochemical Profile of Nile Tilapias (Oreochromis niloticus) Bred in Net Cages during Summer and Winter

Rodrigues

Nascimento

Bizare

et al. 2018

Acta Scientiae. Vet.

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Background: The growth of fish farming in Brazil has been noticeable over the past few years, and the Nile tilapia (Oreochromis niloticus) is the most bred species in the country. Studies on the physiology of these animals are necessary so that the production continues to grow and to improve its rates. However, very discrepant data are still found in the area of blood biochemistry. The aim of this study was to evaluate the physiological variations in serum concentrations of proteins, metabolites, electrolytes and serum enzymes in Nile tilapias bred in net cages in Cachoeira Dourada, state of Minas Gerais, Brazil, during summer and winter.Materials, Methods & Results: During the year of 2016, samples from 100 Nile tilapias were collected during summer (January and February) and samples from 100 Nile tilapias were also collected during winter (July and August). Only one blood sample was collected by caudal venipuncture of each fish, just before the slaughter, always in the morning. The serum samples obtained were then analyzed for serum concentrations of proteins, metabolites, electrolytes, lipids and serum enzymes. Serum concentrations of total proteins, globulins, triglycerides, high density lipoprotein (HDL), alkaline phosphatase, sodium and chloride were significantly higher during winter in comparison to summer, while the serum concentration of uric acid was significantly lower during winter in comparison to summer.Discussion: Due to the lower temperature of water during winter, tilapias reduced food intake and became more susceptible to diseases, which led to an increase in circulating globulins. In that same season, there was an increase in serum concentrations of sodium and chloride as a result of adding sodium chloride to the water in order to stimulate mucus secretion on the fishes’ skin and gills to prevent pathologies. Triglycerides, HDL, and uric acid are considered indicators of nutritional status of animals. The increase in serum concentrations of triglycerides and HDL indicates that tilapias are mobilizing energy reserves, however, when the increase in serum concentrations of uric acid is observed, it is fair to assume there has been an increase in food intake, raising protein catabolism. The increase of serum concentration in alkaline phosphatase is caused by the presence of multiple forms of enzymes that are induced by the action of endogenous corticosteroids, which are produced as a result of the stress suffered by tilapias during the fishing process and during winter, when the water temperature is lower than the ideal for this species. It can be concluded that the season has influence on some serum biochemical constituents of Nile tilapias bred in net cages, and that the predominant variations happen during winter due to immunity decline and during summer due to the increase in food intake and, consequently, in the metabolism of tilapias.

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Evidence for the Role of a Na+/HCO3−Cotransporter in Trout Hepatocyte pHi Regulation

Furimsky

Moon

Perry

2000

Journal of Experimental Biology

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The mechanisms of intracellular pH (pHi) regulation were examined in hepatocytes of the rainbow trout Oncorhynchus mykiss. pHi was monitored using the pH-sensitive fluorescent dye BCECF, and the effects of various media and pharmacological agents were examined for their influence on baseline pHi and recovery rates from acid and base loading. Rates of Na(+) uptake were measured using (22)Na, and changes in membrane potential were examined using the potentiometric fluorescent dye Oxonol VI. The rate of proton extrusion following acid loading was diminished by the blockade of either Na(+)/H(+) exchange (using amiloride) or anion transport (using DIDS). The removal of external HCO(3)(−) and the abolition of outward K(+) diffusion by the channel blocker Ba(2+) also decreased the rate of proton extrusion following acid load. Depolarization of the cell membrane with 50 mmol l(−)(1) K(+), however, did not affect pHi. The rate of recovery from base loading was significantly diminished by the blockade of anion transport, removal of external HCO(3)(−) and, to a lesser extent, by blocking Na(+)/H(+) exchange. The blockade of K(+) conductance had no effect. The decrease in Na(+) uptake rate observed in the presence of the anion transport blocker DIDS and the DIDS-sensitive hyperpolarization of membrane potential during recovery from acid loading suggest that a Na(+)-dependent electrogenic transport system is involved in the restoration of pHi after intracellular acidification. The effects on baseline pHi indicate that the different membrane exchangers are tonically active in the maintenance of steady-state pHi. This study confirms the roles of a Na(+)/H(+) exchanger and a Cl(−)/HCO(3)(−) exchanger in the regulation of trout hepatocyte pHi and provides new evidence that a Na(+)/HCO(3)(−) cotransporter contributes to pHi regulation.

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Intracellular ion levels in erythrocytes and hepatocytes isolated from three teleost species

Cited by 3 publications

References 30 publications

PARÂMETROS BIOQUÍMICOS DE Piaractus mesopotamicus E Colossoma macropomum (CHARACIDAE) E HÍBRIDO TAMBACU (P. mesopotamicus x C. macropomum)

PARÂMETROS BIOQUÍMICOS DE Piaractus mesopotamicus E Colossoma macropomum (CHARACIDAE) E HÍBRIDO TAMBACU (P. mesopotamicus x C. macropomum)

Serum Biochemical Profile of Nile Tilapias (Oreochromis niloticus) Bred in Net Cages during Summer and Winter

Evidence for the Role of a Na+/HCO3−Cotransporter in Trout Hepatocyte pHi Regulation

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