Intracellular hyaluronic acid-binding protein 4 (HABP4): a candidate tumor suppressor in colorectal cancer

Melo‐Hanchuk, Talita Diniz; Colleti, Carolina; Saito, Ângela; Mendes, Maria Carolina Santos; Carvalheira, José Barreto Campello; Vassallo, José; Kobarg, Jörg

doi:10.18632/oncotarget.27804

Cited by 4 publications

(4 citation statements)

References 38 publications

(39 reference statements)

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“…After the formation of DNA heteroduplexes, they were incubated with 5 U of T7EI enzyme (New England Biolabs) at 37 °C for 30 min. Then, the reaction was resolved in a 1% agarose gel electrophoresis [ 22 ]. Purified PCR amplicons digested by ScaI enzyme were also submitted to Sanger sequencing to confirm the insertion of desired mutations.…”

Section: Methodsmentioning

confidence: 99%

Obesity-Linked PPARγ Ser273 Phosphorylation Promotes Beneficial Effects on the Liver, despite Reduced Insulin Sensitivity in Mice

et al. 2023

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Since the removal of thiazolidinediones (TZDs) from the market, researchers have been exploring alternative anti-diabetic drugs that target PPARγ without causing adverse effects while promoting insulin sensitization by blocking serine 273 phosphorylation (Ser273 or S273). Nonetheless, the underlying mechanisms of the relationship between insulin resistance and S273 phosphorylation are still largely unknown, except for the involvement of growth differentiation factor (GDF3) regulation in the process. To further investigate potential pathways, we generated a whole organism knockin mouse line with a single S273A mutation (KI) that blocks the occurrence of its phosphorylation. Our observations of KI mice on different diets and feeding schedules revealed that they were hyperglycemic, hypoinsulinemic, presented more body fat at weaning, and presented an altered plasma and hepatic lipid profile, distinctive liver morphology and gene expression. These results suggest that total blockage of S273 phosphorylation may have unforeseen effects that, in addition to promoting insulin sensitivity, could lead to metabolic disturbances, particularly in the liver. Therefore, our findings demonstrate both the beneficial and detrimental effects of PPAR S273 phosphorylation and suggest selective modulation of this post translational modification is a viable strategy to treat type 2 diabetes.

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Section: Methodsmentioning

confidence: 99%

Obesity-Linked PPARγ Ser273 Phosphorylation Promotes Beneficial Effects on the Liver, despite Reduced Insulin Sensitivity in Mice

et al. 2023

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“…An elevated transcript level of PHBP was found in lung adenocarcinomas [103], and the upregulation of PHBP was also reported to be related to pathological status in non-small-cell lung cancer [70,104]. Both HABP2 and HABP4 seem to be tumour inhibitors [106,158]. Among the lecticans, BCAN overexpression is associated with glioma progression [159].…”

Section: Deregulated Haims In Solid Tumoursmentioning

confidence: 99%

Hyaluronic Acid Interacting Molecules Mediated Crosstalk between Cancer Cells and Microenvironment from Primary Tumour to Distant Metastasis

Xu,

Benedikt,

2024

Cancers

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Hyaluronic acid (HA) is a prominent component of the extracellular matrix, and its interactions with HA-interacting molecules (HAIMs) play a critical role in cancer development and disease progression. This review explores the multifaceted role of HAIMs in the context of cancer, focusing on their influence on disease progression by dissecting relevant cellular and molecular mechanisms in tumour cells and the tumour microenvironment. Cancer progression can be profoundly affected by the interactions between HA and HAIMs. They modulate critical processes such as cell adhesion, migration, invasion, and proliferation. The TME serves as a dynamic platform in which HAIMs contribute to the formation of a unique niche. The resulting changes in HA composition profoundly influence the biophysical properties of the TME. These modifications in the TME, in conjunction with HAIMs, impact angiogenesis, immune cell recruitment, and immune evasion. Therefore, understanding the intricate interplay between HAIMs and HA within the cancer context is essential for developing novel therapeutic strategies. Targeting these interactions offers promising avenues for cancer treatment, as they hold the potential to disrupt critical aspects of disease progression and the TME. Further research in this field is imperative for advancing our knowledge and the treatment of cancer.

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“…found that HABP4 (HABP4), a 57kDa regulatory protein, is involved in tumor transcription regulation and proliferation regulation of colon cancer. After gene knockout, tumor volume increased, indicating that HABP4 is expected to become a new tumor suppressant protein ( 23 ).…”

Section: Application Of the Crispr/cas System In Colon Cancer Researc...mentioning

confidence: 99%

Application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer

Meng

Nan

et al. 2023

Front. Endocrinol.

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Colon cancer is the fourth leading cause of cancer death worldwide, and its progression is accompanied by a complex array of genetic variations. CRISPR/Cas9 can identify new drug-resistant or sensitive mutations in colon cancer, and can use gene editing technology to develop new therapeutic targets and provide personalized treatments, thereby significantly improving the treatment of colon cancer patients. CRISPR/Cas9 systems are driving advances in biotechnology. RNA-directed Cas enzymes have accelerated the pace of basic research and led to clinical breakthroughs. This article reviews the rapid development of CRISPR/Cas in colon cancer, from gene editing to transcription regulation, gene knockout, genome-wide CRISPR tools, therapeutic targets, stem cell genomics, immunotherapy, metabolism-related genes and inflammatory bowel disease. In addition, the limitations and future development of CRISPR/Cas9 in colon cancer studies are reviewed. In conclusion, this article reviews the application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer.

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Intracellular hyaluronic acid-binding protein 4 (HABP4): a candidate tumor suppressor in colorectal cancer

Cited by 4 publications

References 38 publications

Obesity-Linked PPARγ Ser273 Phosphorylation Promotes Beneficial Effects on the Liver, despite Reduced Insulin Sensitivity in Mice

Obesity-Linked PPARγ Ser273 Phosphorylation Promotes Beneficial Effects on the Liver, despite Reduced Insulin Sensitivity in Mice

Hyaluronic Acid Interacting Molecules Mediated Crosstalk between Cancer Cells and Microenvironment from Primary Tumour to Distant Metastasis

Application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer

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