Application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer

Meng, Hui; Nan, Man-Man; Li, Yizhen; Ding, Yi; Yi, Yin; Zhang, Mingzhi

doi:10.3389/fendo.2023.1148412

Cited by 10 publications

(9 citation statements)

References 166 publications

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“…Xia et al. discovered that MARCH 2 was increasingly expressed in colon cancer with a poorer prognosis and lower survival rate ( 49 ). MARCH 2 was knocked out, and the endoplasmic reticulum stress was stimulated with the inhibition of cancer cell growth and induction of apoptosis ( 50 ).…”

Section: Survey Methodologymentioning

confidence: 99%

“…FAPP2 was knocked by CRISPR/Cas9 technology, the cell growth of tumors was blocked, and the tumorigenicity was decreased ( 51 ). CLCA1 was knocked out using the CRISPR system ( 49 ). After knocking out CLCA1, the proliferation, and migration of colon cancer tumor cells were increased, and the high level of expression of CLCA1 caused the inhibition of the Wnt signaling transduction and reduced the epithelial-mesenchymal-transition (EMT) process that blocked tumor growth ( 52 ).…”

Section: Survey Methodologymentioning

confidence: 99%

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The use of plant-derived exosome-like nanoparticles as a delivery system of CRISPR/Cas9-based therapeutics for editing long non-coding RNAs in cancer colon cells

Hillman¹

2023

Front. Oncol.

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Colon cancer is one of the leading causes of cancer in the United States. Colon cancer develops from the many gene mutations found in the genomes of colon cancer cells. Long non-coding RNAs (lncRNAs) can cause the development and progression of many cancers, including colon cancer. LncRNAs have been and could be corrected through the gene-editing technology of the clustered repeats of the clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) system to reduce the proliferation of cancer cells in the colon. However, many current delivery systems for transporting CRISPR/Cas9-based therapeutics in vivo need more safety and efficiency. CRISPR/Cas9-based therapeutics require a safe and effective delivery system to more directly and specifically target cancer cells present in the colon. This review will present pertinent evidence for the increased efficiency and safety of using plant-derived exosome-like nanoparticles as nanocarriers for delivering CRISPR/Cas9-based therapeutics to target colon cancer cells directly.

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Section: Survey Methodologymentioning

confidence: 99%

Section: Survey Methodologymentioning

confidence: 99%

The use of plant-derived exosome-like nanoparticles as a delivery system of CRISPR/Cas9-based therapeutics for editing long non-coding RNAs in cancer colon cells

Hillman¹

2023

Front. Oncol.

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“…CRISPR‐Cas9 system was investigated in colon cancer epigenetic therapy. [ 36 ] HIV‐based lentiviral vectors: origin and sequence differences…”

Section: Genome Engineering Technologies For Cancer Treatmentmentioning

confidence: 99%

Genome Engineering as a Therapeutic Approach in Cancer Therapy: A Comprehensive Review

Gemayel,

Chebly,

Kourie

et al. 2024

Advanced Genetics

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Cancer is one of the foremost causes of mortality. The human genome remains stable over time. However, human activities and environmental factors have the power to influence the prevalence of certain types of mutations. This goes to the excessive progress of xenobiotics and industrial development that is expanding the territory for cancers to develop. The mechanisms involved in immune responses against cancer are widely studied. Genome editing has changed the genome‐based immunotherapy process in the human body and has opened a new era for cancer treatment. In this review, recent cancer immunotherapies and the use of genome engineering technology are largely focused on.

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“…Consequently, these results provide valuable insights into the genetic mechanisms involved in these cancers and suggest that these genes could serve as potential targets for the development of novel therapies. Therefore, the CRISPR-based gene editing technology used in the Achilles project has the potential to provide valuable insights for developing new cancer treatments and ultimately improving outcomes for cancer patients 32,[47][48][49][50][51][52][53][54][55] .…”

Section: Correlation Between Gene Essentiality and Mrna Expression Of...mentioning

confidence: 99%

Shedding Light on the Dark Genome: Insights into the Genetic, CRISPR-based, and Pharmacological Dependencies of Human Cancers and Disease Aggressiveness

Kafita,

Nkhoma,

Dzobo

et al. 2023

Preprint

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Investigating the human genome is vital for identifying risk factors and devising effective therapies to combat genetic disorders and cancer. Despite the extensive knowledge of the "light genome", the poorly understood "dark genome" remains understudied. In this study, we integrated data from 20,412 protein-coding genes in Pharos and 8,395 patient-derived tumours from The Cancer Genome Atlas (TCGA) to examine the genetic and pharmacological dependencies in human cancers and their treatment implications. We discovered that dark genes exhibited high mutation rates in certain cancers, similar to light genes. By combining the drug response profiles of cancer cells with cell fitness post-CRISPR-mediated gene knockout, we identified the crucial vulnerabilities associated with both dark and light genes. Our analysis also revealed that tumours harbouring dark gene mutations displayed worse overall and disease-free survival rates than those without such mutations. Furthermore, dark gene expression levels significantly influenced patient survival outcomes. Our findings demonstrated a similar distribution of genetic and pharmacological dependencies across the light and dark genomes, suggesting that targeting the dark genome holds promise for cancer treatment. This study underscores the need for ongoing research on the dark genome to better comprehend the underlying mechanisms of cancer and develop more effective therapies.

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Application of CRISPR-Cas9 gene editing technology in basic research, diagnosis and treatment of colon cancer

Cited by 10 publications

References 166 publications

The use of plant-derived exosome-like nanoparticles as a delivery system of CRISPR/Cas9-based therapeutics for editing long non-coding RNAs in cancer colon cells

The use of plant-derived exosome-like nanoparticles as a delivery system of CRISPR/Cas9-based therapeutics for editing long non-coding RNAs in cancer colon cells

Genome Engineering as a Therapeutic Approach in Cancer Therapy: A Comprehensive Review

Shedding Light on the Dark Genome: Insights into the Genetic, CRISPR-based, and Pharmacological Dependencies of Human Cancers and Disease Aggressiveness

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