Intracellular growth in vitro of Trypanosoma (Schizotrypanum) dionisii of bats and preliminary work on cell-mediated immunity using this system

Baker, John; Green, Sheila M.; Chaloner, Lisbeth A.; Gaborak, Maria

doi:10.1016/0035-9203(72)90221-0

Cited by 11 publications

(5 citation statements)

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“…mice, rats, guinea pigs, hamsters, and rabbits (Petrie 1905;Sergent and Sergent 1905;Bettencourt and Fran~a 1906;Mettam 1907;Gonder 1910;Pringault 19 14;Dias 1933Dias , 1934Dias , 1940Roman& 1944, Hedrick 1955Torrealba et al 1958;Cadena 1967;Molyneux and Bafort 1971;Baker et al 19726;Baker et al 1978;Fahmy et al 1978;Baker and Miles 1979). The infectivity of their inocula to bats were tested by and Molyneux and Bafort (1971).…”

Section: Discussionmentioning

confidence: 94%

“…dionisii (see Baker and Thompson 1971). Most species reported from Eurasia and Africa were either inadequately described or the descriptions were based on few specimens (Battaglia 1904;Bettencourt andFransa 1905, 1906;Sergent and Sergent 1905;Petrie 1905;Nicolle and Comte 1906;Gonder 1910;Cartaya 1910;Breinl 191 1;Coles 1914;Chatton and Courrier 192 1;Franchini 1921;Mackerras 1959;Dunn 1964;Keymer 1971;Baker et al 1972~). More recent descriptions from Africa and those from the New World were reported in more detail (Dias 1940;Dias andPifano 1941, 1942;Hedrick 1955;Zeledon and Vieto 1958;Cadena 1967;Barretto et al 1968;Woo and Hawkins 1975;Fahrny et al 1978).…”

Section: Discussionmentioning

confidence: 98%

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Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

Bower¹,

Woo²

1981

Can. J. Zool.

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Two new species of trypanosomes (subgenus Schizotrypanum) in bats fromsouthern Ontario. Can. J. Zool. 59: 530-545. From November of 1976 to May of 1979, the blood of 529 bats from 12 sites in southern Ontario was examined for trypanosomes using the haematocrit centrifugation technique. Trypanosoma hedricki n.sp. was found in 62 of 216 Eptesicus fuscus and Trypanosoma myoti n.sp. in 16 of 313 Myotis lucifugus. Blood forms of both species were morphologically similar to Trypanosoma cruzi. These trypanosomes were readily cultured in diphasic blood-agar medium.Cultures of T . hedricki n.sp. and T . myoti n.sp. were infective when inoculated orally or injected intraperitoneally into laboratory reared E. fuscus and M. lucifugus respectively. Pseudocysts of amastigotes were found in cardiac muscles of both bat species and in the intestinal smooth muscle of M. lucifugus. Trypanosoma hedricki n.sp. was not infective to M. lucifugus nor was T . myoti n.sp. infective to E. fuscus. Unlike T. cruzi, cultures were not infective to Mus musculus, Peromyscus maniculatus, Microtus pennsylvanicus, Mesocricetus auratus, Rattus norvegicus, and Cavia porcellus.After in vitro incubation in fresh plasma from deer mice, hamsters, laboratory rats, guinea pigs, little brown bats, and Homo sapiens, T . hedricki n.sp. could not be cultured. However, positive cultures were obtained after incubation in fresh plasma from E. fuscus and occasionally in fresh plasma from laboratory mice. Positive cultures were always obtained when the plasma was heat inactivated. BOWER, S. M., et P. T. K. Woo. 1981. Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario. Can. J. Zool. 59: 530-545. De novembre 1976 B mai 1979, le sang de 529 chauves-souris de 12 localitts difftrentes du sud de ]'Ontario a Ctt soumis ? i la technique de centrifugation afin d'y rechercher la presence de trypanosomes. Trypanosoma hedricki n.sp. a Ctt trouvt chez 62 des 216 Eptesicus fuscus examints et Trypanosoma myoti n.sp., chez 16 des 313 Myotis lucifugus. La phase sanguine de ces deux esp&ces est semblable morphologiquement B celle de Trypanosoma cruzi. Ces trypanosomes peuvent facilement &tre gardCs en culture en milieu diphasique sang-agar. Les cultures de T . hedricki n. sp. sont infectieuses lorsqu'elles sont administdes oralement ou par injection intra-abdorninale h des E. fuscus Clevts en laboratoire; de la m6me f a~o n , les cultures de T . myoti sont infectieuses pour des M. lucifugus. Des pseudocystes d'amastigotes se retrouvent dans le muscle cardiaque des deux espbces de chauves-souris et dans le muscle intestinal lisse de M. lucijiugus. Trypanosoma hedricki n.sp. ne parasite pas M, luclfugus et T . myoti n'est pas infectieux chez E. fuscus. Alors que T . cruzi est infectieux chez Mus musculus, Peromyscus maniculatus, Microtus pennsylvanicus, Mesocricetus auratus, Rattus norvegicus et Cavia porcellus, les cultures de T . hedricki et de T . myoti ne le sont pas. Des T. hedricki n.sp. ont t t t rnis en incubation in vitro dans du plasma ...

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

Bower¹,

Woo²

1981

Can. J. Zool.

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“…Baker & Miles (1979) indicated that the measurements of long thin trypomastigotes of T. dionisii breve from cultures were significantly different from those of T. dionisii dionisii. However, shorter trypomastigotes have been reported from T. dionisii in vitro (Baker et al, 1972a(Baker et al, , 1972b). In the present study, T. dionisii, T. hedricki and T. myoti, as well as clones of these species, produced a variety of trypomastigotes.…”

Section: Discussionmentioning

confidence: 99%

“…T. vespertilionis and T. dionisii did not develop in chicken fibroblasts, whereas amastigotes of T. hedrieki and T. myoti did. European workers found that T. dionisii developed better in mammalian macrophages than in fibroblasts (Baker et al, 1972a(Baker et al, , 1972bListon, 1975;Baker & Selden, 1978;Liston & Baker, 1978). More recent work (Baker et al, 1980) showed that cultured trypomastigotes of T. dionisii would also develop in 'buffalo lung cells'.…”

Section: Discussionmentioning

confidence: 99%

“…T. cruzi is also known to develop in chicken and mouse fibroblasts but a comparative study has not been done (Kofoid et al, 1935;Meyer & Xavier de Olivera, 1948). Baker et al (1971Baker et al ( , 1972aBaker et al ( , 1972b suggested that the long thin trypomastigotes of T. dionisii were the forms that invaded fibroblasts in vitro. Blood and culture forms (trypomastigotes, epimastigotes and amastigotes) of T. cruzi are infective to fibroblasts (Dvorak & Schmunis, 1972;Luban & Dvorak, 1974;Pan, 1978).…”

Section: Discussionmentioning

confidence: 99%

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An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

Bower

Woo

1981

Syst Parasitol

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Epimastigotes of Trypanosoma vespertilionis from diphasic blood agar cultures were on the average longer and the distance between the nucleus and kinetoplast greater than epimastigotes of T. hedricki, T. myoti and T. dionisii. Also, no yellow granules were seen in the epimastigotes of T. vespertilionis whereas they were obvious in the other three species. Long thin trypomastigotes which are characteristic of T. hedricki, T. myoti and T. dionisii cultures were not seen in T. vespertilionis.T. dionisii was much less infective to fibroblasts from mice and did not develop in fibroblasts from chicken, as did T. hedricki and T. myoti. Blood trypomastigotes were seen in chicken embryos inoculated with blood agar cultures of T. hedricki and T. myoti, but none was seen in embryos infected with T. dionisii.The cultural characteristics examined could not be used to differentiate T. hedricki from T. myoti.

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An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

Bower¹,

Woo²

1981

Systematic Parasitology

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Intracellular growth in vitro of Trypanosoma (Schizotrypanum) dionisii of bats and preliminary work on cell-mediated immunity using this system

Cited by 11 publications

References 0 publications

Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

Two new species of trypanosomes (subgenus Schizotrypanum) in bats from southern Ontario

An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

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