Intracellular Development of Vaccinia Virus.

Gaylord, William H.; Melnick, Joseph L.; Bunting, Henry

doi:10.3181/00379727-80-19509

Cited by 14 publications

(6 citation statements)

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“…Dawson and McFarlane (10) examined isolated vaccinia virus particles after having treated them with pepsin and found that they contained a dense "nucleus" and a thin membrane surrounding each particle. The substance between the outer limiting membrane of the vinm and the "nucleus" was missing giving a picture of the particle which was exactly the reverse of that seen in the present work for both osmium and formalin (14) fixed mature virus. It would seem that the material removed by the pepsin constitutes the thick wall of our particles.…”

Section: Ectromelia-infected Cells Of the Chorioallantois--ectromelisupporting

confidence: 64%

“…The basement membrane was apparently not broken and no virus was seen in the mesoderm or entoderm. The ectoderm contained much more virus than at 48 hours (14) and individual cells were more heavily infected. It was, nevertheless, possible to find cells in all stages of involvement.…”

Section: Vacclnia-infected Cells Of the Chorioallantois--mter 72 Houmentioning

confidence: 99%

“…Vaccinia-infected chorioallantois has been investigated by Bang (5) who suggested a relationship between mitochondria and virus, and by Wyckoff (31) who pointed out certain structures of low electron density which might have a role in virus development. Using similar material, we have called attention to the perinuclear position of early vaccinia virus development and to a matrix enveloping the earliest forms (13,14). Molluscum contagiosum virus has been identified in thin sections of the human lesion in two laboratories (4,9).…”

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confidence: 99%

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Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Gaylord

Melnick

1953

Journal of Experimental Medicine

103

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The intracellular development of three pox viruses has been studied with the electron microscope using thin sections of infected tissue. Cells infected with vaccinia, ectromelia, and molluscum contagiosum viruses all form developmental bodies preliminary to the production of mature virus. Developmental bodies, believed to be virus precursors, are round to oval, slightly larger than mature virus particles, less dense to electrons, and have a more varied morphology. It is suggested as a working hypothesis that the process of maturation of a virus particle takes place as follows. In the earliest form the developmental bodies appear as hollow spheres, imbedded in a very dense cytoplasmic mass constituting an inclusion body, or in a less dense matrix near the nucleus in cells without typical inclusion bodies. The spheres become filled with a homogeneous material of low electron density. A small, dense granule appears in each developmental body and grows in size at the expense of the low density material. Following growth of the granule, particles are found with the dimensions of mature virus and having complex internal structure resembling bars or dumbells. Mature virus is ovoid and very dense to electrons. An "empty" interior may be found within its thick walls.

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Section: Ectromelia-infected Cells Of the Chorioallantois--ectromelisupporting

confidence: 64%

Section: Vacclnia-infected Cells Of the Chorioallantois--mter 72 Houmentioning

confidence: 99%

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confidence: 99%

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Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Gaylord

Melnick

1953

Journal of Experimental Medicine

103

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“…This does not seem to be confined to the Luck6 virus. In an electron microscope study of the intracellular forms of the viruses of vaccinia, ectrome]ia, and molluscum contagiosum, Gaylord and Melnick (13,14) noted the occurrence of numerous complete membranes forming hollow spheres that were apparently empty. Bernhard also reported that it was not uncommon in the cytoplasmic inclusion bodies of the Shope fibroma to find complete membranes forming spheres that were seemingly devoid of contents.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscope Observations on Intracellular Virus-Like Particles Associated With the Cells of the Lucké Renal Adenocarcinoma

Fawcett

1956

The Journal of Cell Biology

166

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Relatively few malignant neoplasms have been described in amphibia. The one that has been studied most extensively is the Luck6 renal adenocarcinoma of the frog, Rana pipiens. This malignant tumor occurs in from two to four per cent of frogs of this species, collected in the Lake Champlain region of northern Vermont and adjoining areas of Quebec. In a series of papers Luck6 described its histopathology (17), reported the occurrence of distant metastases (18) and demonstrated the presence in some of the tumors of intranuclear inclusion bodies of a kind commonly seen in virus diseases (17,22). It was subsequently shown by him that tumors could be induced in frogs of the susceptible race by injection of desiccated or glycerinated preparations of tumor. Similar inoculation of other races or species was without effect. These observations led Luck6 to conclude that the tumors were probably caused by a species-specific, organspecific agent having the characteristics of a virus (19,20). Additional supporting evidence for this conclusion was provided in later work of Schlulnberger and Luck6 (31) in which the tumor was carried for many generations by serial intraocular transplantation. In the course of these experiments it was noted that frogs bearing intraocular tumors very often developed kidney tumors. Similar results have been reported by Rose and Rose (30) in anterior chamber transplants in adult frogs, and by Briggs (4) in tadpoles with tumor implants in the tail fin. In both instances persistently growing adenocarcinomas frequently developed in the kidneys even though the implanted tumor tissue itself regressed. The most reasonable interpretation of these findings seems to be that a tumor agent passed from the implanted tissue to the kidney and there induced a tumor of the same kind. Further evidence of the viral nature of the tumor-inducing material has been provided by the work of Duryee and Doherty (8, 9) who have recently established its filtrability.In the present investigation, thin sections of frog renal tumors have been

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“…The use of electron microscopy for examination of the cytoplasmic inclusions of these large viruses has usually, but not always, shown the presence of viruslike particles within them. With the earlier techniques of electron microscopy, viruslike particles were seen in the inclusion bodies of ectromelia (49), vaccinia (50,51), moUnscum contagiosum (52), and probably fowl pox (53), but recent work with unshadowed sections after osmium fixation appears to give better results and viral particles can be identified with greater certainty. Under these conditions, the inclusion bodies of molluscum contagiosum showed vires-like particles (44).…”

Section: While the Presence Of Inclusion Bodies Within Cells Does Notmentioning

confidence: 99%

Electron Microscopy of Early Cytoplasmic Changes Due to Influenza Virus

Harford

Hamlin

Parker

1955

The Journal of Experimental Medicine

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Recently improved methods for visualization of thin tissue sections by electron microscopy have been applied to the study of early changes in the bronchial epithelium of mice infected by inhalation of aerosols of influenza virus. In confirmation of previous findings by the authors, inclusion bodies have been demonstrated in ciliated and non-ciliated cells of infected bronchial epithelium. In addition to 3 strains of mouse-adapted Type A virus, 2 unadapted strains gave qualitatively the same results. The inclusion bodies were found to be composed largely of particles of a size estimated to correspond to the known size of influenza virus. The viral lesion of the cytoplasm was also associated with linear formations which were thought to be abnormal forms of endoplasmic reticulum. Well developed microvilli were found on the ciliated borders of ciliated cells, but no evidence was found of viral growth in this region.

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Intracellular Development of Vaccinia Virus.

Cited by 14 publications

References 0 publications

Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Electron Microscope Observations on Intracellular Virus-Like Particles Associated With the Cells of the Lucké Renal Adenocarcinoma

Electron Microscopy of Early Cytoplasmic Changes Due to Influenza Virus

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