Electron Microscope Observations on Intracellular Virus-Like Particles Associated With the Cells of the Lucké Renal Adenocarcinoma

Abstract

Relatively few malignant neoplasms have been described in amphibia. The one that has been studied most extensively is the Luck6 renal adenocarcinoma of the frog, Rana pipiens. This malignant tumor occurs in from two to four per cent of frogs of this species, collected in the Lake Champlain region of northern Vermont and adjoining areas of Quebec. In a series of papers Luck6 described its histopathology (17), reported the occurrence of distant metastases (18) and demonstrated the presence in some of the tumors of intranuclear inclusion bodies of a kind commonly seen in virus diseases (17,22). It was subsequently shown by him that tumors could be induced in frogs of the susceptible race by injection of desiccated or glycerinated preparations of tumor. Similar inoculation of other races or species was without effect. These observations led Luck6 to conclude that the tumors were probably caused by a species-specific, organspecific agent having the characteristics of a virus (19,20). Additional supporting evidence for this conclusion was provided in later work of Schlulnberger and Luck6 (31) in which the tumor was carried for many generations by serial intraocular transplantation. In the course of these experiments it was noted that frogs bearing intraocular tumors very often developed kidney tumors. Similar results have been reported by Rose and Rose (30) in anterior chamber transplants in adult frogs, and by Briggs (4) in tadpoles with tumor implants in the tail fin. In both instances persistently growing adenocarcinomas frequently developed in the kidneys even though the implanted tumor tissue itself regressed. The most reasonable interpretation of these findings seems to be that a tumor agent passed from the implanted tissue to the kidney and there induced a tumor of the same kind. Further evidence of the viral nature of the tumor-inducing material has been provided by the work of Duryee and Doherty (8, 9) who have recently established its filtrability.In the present investigation, thin sections of frog renal tumors have been

Unusual Intranuclear Tubular Structures Associated with the Maturation of Herpesvirus saimiri in Monkey Kidney Cell Cultures

“…Where negative contrast preparations have been studied this idea has been supported by the finding of spiral arrays of capsomeres on the surface of the tubules, identical to those covering the immature virus particles (Stackpole and Mizell, 1968). However, the other herpesvirus-associated structures do not seem to be related to recognizable virus components since they are either altered spindle tubules (Fawcett, 1956;Epstein, Achong and Barr, 1964), or membranes within the nucleoplasm (Epstein et al, 1968;Nii et al, 1968), or microtubular lattices (Murphy et al, 1967;Couch and Nahmias, 1969), or fibrils accompanied by repeating sub-units (Chitwood and Bracken, 1964;Campbell and Woode, 1970).…”

“…THE development of a number of herpesviruses is often accompanied by the appearance of one or other of a variety of bizarre tubular or membranous structures in the infected cells (Fawcett, 1956;Watrach, 1962;Chitwood and Bracken, 1964;Epstein, Achong and Barr, 1964; Murphy, Harrison and Whitfield, 1967;Epstein et al, 1968;Nii, Morgan and Rose, 1968;Stackpole and Mizell, 1968;Couch and Nahmias, 1969;Campbell and Woode, 1970;Heine and Hinze, 1972;McKinnell and Ellis, 1972). With infectious laryngotracheitis virus, Marek's disease herpesvirus, and the Lucke tumour herpesvirus such tubular structures found in the nucleus have been likened, when sectioned in certain planes for electron microscopy, to linear assemblies resembling immature virus particles in construction (Watrach, 1962;Stackpole and Mizell, 1968;Campbell and Woode, 1970), although somewhat variable in cross section and elongated.…”

Electron Microscopic Observations on the Development of Herpes Simplex Virus

“…Stoker, Smith, and Ross (2) confirmed the presence of intranuclear particles with double membranes in tissue cultures infected with the HFEM strain. Similar observations have been made during electron microscopic examination of two viruses which appear morphologically to resemble herpes simplex virus, viz., the virus associated with renal adenocarcinoma of frogs (31) and varicella virus (32). In the last two instances , as well as in a study of salivary gland virus (33), collections of intracytoplasmic viral particles were found within vacuoles.…”

Karyotype Analysis of a Frog Pronephric Tumor Cell Line

“…Substantial evidence has accumulated from electron microscopy [7,13] indicating the presence and replication of Lucké herpes virus (LHV) in the nuclear inclusion or 'winter' tumor. Transmission by inoculation of embryos with LHV de rived from winter tumor cell fractions produces pronephric or meso nephric renal carcinomas in larva or metamorphosing adults [15,17,18,23].…”