Intracellular Delivery of Bak BH3 Peptide by Microbubble-Enhanced Ultrasound

Kinoshita, Manabu; Hynynen, Kullervo

doi:10.1007/s11095-005-2586-7

Cited by 50 publications

(27 citation statements)

References 24 publications

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“…2C and D). This is a similar observation compared to what we have previously seen in cells sonicated in a suspension [30,31]. Delivery of siRNA against EGFP into cells with cell attached on the culture dish was also demonstrated possible ( Fig.…”

Section: Resultssupporting

confidence: 87%

“…However, when we look into the published reports, sonoporation efficiency and the magnitude of cell toxicity, one of the most important factors for making sonoporation as a practical tool, varies in a very wide range. Some reports, including our previous reports, show only a small amount of successfully sonoporated cells accompanying a large number of non-viable cells [25,26,30,31,35]. Others report otherwise [34,38].…”

Section: Introductionmentioning

confidence: 74%

“…Although there are many reports on sonoporation [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], each experimental setting differs from one another and the reported sonoporation efficiency, which is one of the most significant information in sonoporation, varies. In order to understand the effect of the experimental settings themselves on sonoporation experiments, we first investigated the impact of the conditions of the cultured cells on sonoporation efficiency.…”

Section: Resultsmentioning

confidence: 99%

“…(Current address: Department of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Oaska, 565-0871, Japan. Phone: +81-6-6879-3652: FAX: +81-6-6879-3659; e-mail : m-kinoshita@nsurg.med.osaka-u.ac.jp) hyperthermia, such as coagulation of tissues [1][2][3][4][5][6][7][8][9][10][11][12], mechnical tissue destruction [13,14], disruption of tight junctions of the vasculature for enhanced local drug delivery [15][16][17][18][19][20], and sonoporation, a transient enhanced permeability of the cell membrane caused by the combination of ultrasound and microbubbles [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. As all of these bio-effects can be created non-invasively in vivo, ultrasound is gathering a great attention as a next generation surgical or drug delivery system.…”

Section: Introductionmentioning

confidence: 99%

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Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Kinoshita

Hynynen

2007

Biochemical and Biophysical Research Communications

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106

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Ultrasound induced intracellular drug delivery, sonoporation, is an appealing and promising technique for next generation drug delivery system. Many types of molecules, such as plasmid DNAs, siRNAs and peptides, have been demonstrated to be delivered into the cell by ultrasound with the aid of microbubbles both in vitro and in vivo. Although there are many reports on in vitro sonoporation, the efficiency of successful sonoporation and the viabilities of cells after the procedure documented in each report vary in a wide range, and the reasons for these differences are not fully understood. In this study, we have investigated how different experimental settings would affect sonoporation efficiency and cell viabilities after the procedure. Our results show that the fashion of cell culture (e.g. in suspension or in monolayer culture) and the presence of standing wave have a great impact on the overall results. These results indicate that in vitro sonoporation settings should be carefully evaluated in each experiment. The fact that standing wave is necessary to achieve high sonoporation efficiency may be a problematic issue for clinical application of sonoporation, as it may be difficult (although not impossible) to create standing wave in a human body.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Kinoshita

Hynynen

2007

Biochemical and Biophysical Research Communications

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106

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“…The incorporation of the marker inside the phospholipid bilayer provides a more generic approach for labeling a variety of possible drug carriers such as micelles [21][22][23], acoustically active lipospheres [24] and microbubbles [25][26][27][28][29][30] …”

mentioning

confidence: 99%

Long-circulating liposomes radiolabeled with [18F]fluorodipalmitin ([18F]FDP)

Mařı́k

Tartis

Zhang

et al. 2007

Nuclear Medicine and Biology

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Synthesis of a radiolabeled diglyceride 3-[ 18 F]fluorodipalmitoyl-1,2-glycerol ( 18 F-fluorodipalmitin, [ 18 F]FDP) and its potential as a reagent for radiolabeling long-circulating liposomes were investigated. The incorporation of 18 F into the lipid molecule was accomplished by nucleophilic substitution of p-toluenesolfonyl moiety with a decay corrected yield of 43 ± 10% (n = 12). Radiolabeled long-circulating PEG-coated liposomes were prepared using a mixture of DPPC, cholesterol, DSPE-PEG2000 (61:30:9) and [ 18 F]FDP with a decay corrected yield of 70 ± 8% (n = 4). PET imaging and biodistribution studies were performed with free [ 18 Liposomes are vesicles composed of one or more concentric phospholipid bi-layers and such vesicles have been widely investigated as possible drug carriers [1,2]. Prolonged blood circulation of the liposomes is achieved with the addition of a polyethylene glycol (PEG) coating, which efficiently minimizes their removal by macrophages of the reticuloendothelial system [3][4][5][6]. Liposomes with various target-specific ligands attached to their surface are being investigated for targeted drug delivery [1,2,7]. Liposomes labeled with radioisotopes such as 99m Tc, 186 Re, 67 Ga, 111 In, and 18 F were previously employed to study the biodistribution of different types of liposomes in various animal models using scintigraphy, SPECT and PET. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Materials and Methods GeneralThe solvents and chemicals were purchased from Aldrich (Milwaukee, WI). The 1 H and 13 C NMR spectra were recorded using a Bruker Avance 500 spectrometer and the chemical shifts are reported relative to TMS. Analytical reversed-phase HPLC was performed using a Phenomenex Jupiter 5μ C4 300A column ( . The size distribution of liposomes was determined using a particle size analyzer Nanotrac NPA150 purchased from Microtrac Inc. (North Largo, FL). The lipid concentration was determined using a Phospholipids B kit purchased from Wako Chemicals, Inc. (Richmond, VA). Synthesis of 3-tosyl-1,2-dipalmitoyl glycerol (2)The precursor 2 was prepared according to the previously published procedure [32] from 1,2-dipalmitoyl-sn-glycerol (1) and 4-toluenesulfonyl chloride. The crude product was subsequently purified by recrystalization from hexane. 1 mL). The content of the vial was dissolved in anhydrous acetonitrile (0.35 mL) and transferred into a suspension of 2 (5.5 mg) in anhydrous acetonitrile (0.5 mL). The reaction mixture was heated to 100 ºC for 20 minutes and allowed to cool to room temperature for 5 min...

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Ultrasound imaging beyond the vasculature with new generation contrast agents

Perera

Hernandez

Zhou

et al. 2015

WIREs Nanomed Nanobiotechnol

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Current commercially available ultrasound contrast agents are gas-filled, lipid- or protein-stabilized microbubbles larger than 1 μm in diameter. Because the signal generated by these agents is highly dependent on their size, small yet highly echogenic particles have been historically difficult to produce. This has limited the molecular imaging applications of ultrasound to the blood pool. In the area of cancer imaging, microbubble applications have been constrained to imaging molecular signatures of tumor vasculature and drug delivery enabled by ultrasound-modulated bubble destruction. Recently, with the rise of sophisticated advancements in nanomedicine, ultrasound contrast agents, which are an order of magnitude smaller (100-500 nm) than their currently utilized counterparts, have been undergoing rapid development. These agents are poised to greatly expand the capabilities of ultrasound in the field of targeted cancer detection and therapy by taking advantage of the enhanced permeability and retention phenomenon of many tumors and can extravasate beyond the leaky tumor vasculature. Agent extravasation facilitates highly sensitive detection of cell surface or microenvironment biomarkers, which could advance early cancer detection. Likewise, when combined with appropriate therapeutic agents and ultrasound-mediated deployment on demand, directly at the tumor site, these nanoparticles have been shown to contribute to improved therapeutic outcomes. Ultrasound's safety profile, broad accessibility and relatively low cost make it an ideal modality for the changing face of healthcare today. Aided by the multifaceted nano-sized contrast agents and targeted theranostic moieties described herein, ultrasound can considerably broaden its reach in future applications focused on the diagnosis and staging of cancer.

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Intracellular Delivery of Bak BH3 Peptide by Microbubble-Enhanced Ultrasound

Abstract: Our results suggest that microbubble-enhanced focused ultrasound peptide transduction is possible. Further optimization of ultrasound exposure conditions may be necessary.

Cited by 50 publications

References 24 publications

Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Key factors that affect sonoporation efficiency in in vitro settings: The importance of standing wave in sonoporation

Long-circulating liposomes radiolabeled with [18F]fluorodipalmitin ([18F]FDP)

Ultrasound imaging beyond the vasculature with new generation contrast agents

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