Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction

Watanapokasin, Yuwadee; Tananyutthawongese, Chantra; Uthaisang, Wanlaya; Chansiri, Kosum; Boonmatit, Chaowalit; Sarataphan, Nopporn

doi:10.1016/s0304-4017(98)00145-9

Cited by 11 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even its distinction from T. equiperdum is sometimes questioned [52, 53]. The Trypanozoon subgenus constitutes a homogeneous group [61] and, especially inside T. evansi , most authors demonstrate a high molecular homogeneity [62], even though some reports state surprising heterogeneity at strain level [63, 64]. …”

Section: Disease Synonyms and Parasite Taxonomymentioning

confidence: 99%

Trypanosoma evansiand Surra: A Review and Perspectives on Origin, History, Distribution, Taxonomy, Morphology, Hosts, and Pathogenic Effects

Desquesnes

Holzmüller

Lai

et al. 2013

BioMed Research International

320

327

View full text Add to dashboard Cite

Trypanosoma evansi, the agent of “surra,” is a salivarian trypanosome, originating from Africa. It is thought to derive from Trypanosoma brucei by deletion of the maxicircle kinetoplastic DNA (genetic material required for cyclical development in tsetse flies). It is mostly mechanically transmitted by tabanids and stomoxes, initially to camels, in sub-Saharan area. The disease spread from North Africa towards the Middle East, Turkey, India, up to 53° North in Russia, across all South-East Asia, down to Indonesia and the Philippines, and it was also introduced by the conquistadores into Latin America. It can affect a very large range of domestic and wild hosts including camelids, equines, cattle, buffaloes, sheep, goats, pigs, dogs and other carnivores, deer, gazelles, and elephants. It found a new large range of wild and domestic hosts in Latin America, including reservoirs (capybaras) and biological vectors (vampire bats). Surra is a major disease in camels, equines, and dogs, in which it can often be fatal in the absence of treatment, and exhibits nonspecific clinical signs (anaemia, loss of weight, abortion, and death), which are variable from one host and one place to another; however, its immunosuppressive effects interfering with intercurrent diseases or vaccination campaigns might be its most significant and questionable aspect.

show abstract

Section: Disease Synonyms and Parasite Taxonomymentioning

confidence: 99%

Trypanosoma evansiand Surra: A Review and Perspectives on Origin, History, Distribution, Taxonomy, Morphology, Hosts, and Pathogenic Effects

Desquesnes

Holzmüller

Lai

et al. 2013

BioMed Research International

320

327

View full text Add to dashboard Cite

show abstract

“…Previously, the intra-species genetic variation of Trypanosoma have been reported in T. brucei [2], T. congolense [30], and T. evansi [33,46] in different hosts basically revealed by PCR based method. Herein, we investigated the diversity of T. evansi in a single host (buffalo) using the nucleotide data of 18S rDNA, 5.8S rDNA, and ITS regions.…”

mentioning

confidence: 99%

Genetic Diversity of Trypanosoma evansi in Buffalo based on Internal Transcribed Spacer (ITS) Regions

Khuchareontaworn

Singhaphan

Viseshakul

et al. 2007

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The nucleotide sequences of 18S rDNA and internal transcribed spacer (ITS) regions were used for studying the relationships of Trypanosoma evansi isolate from a buffalo. The sequences were analyzed and compared to 18S rDNA and the ITS regions of the other Trypanosoma spp. Maximum likelihood phylogenetic trees were constructed using Leishmania major as the outgroup. The tree of 18S rDNA indicated that T. evansi (buffalo B18) isolate was closely related to those of Taiwan and T. brucei stock. The ITS tree showed the genetic diversity among 32 clones of T. evansi (B18) within a single host. This data will be useful for epidemiological and dynamic studies for designing the rational control programs of the disease. KEY WORDS: internal transcribed spacers (ITS), phylogenetic, small subunit rDNA, Trypanosoma evansi.

show abstract

“…Alternatively, the random amplified polymorphic dna (rapd) technique (Williams and others 1990, Welsh and McClelland 1991) requires only a small amount of dna and no prior knowledge of the genome of the organisms for detecting genetic variation. Waitumbi and others (1994) used this rapd technique to study the genotype and drug‐resistant phenotypes of T evansi , and Watanapokasin and others (1998) used it successfully to discern intraspecies differentiation of the organisms in cattle.…”

Section: Primer Sequence Length (Number Of Nucleotides) Guanine and Cmentioning

confidence: 99%

“…A limited heterogeneity in the stocks for T evansi was earlier reported on the basis of isoenzyme analysis (Gibson and others 1983, Boid 1988), k dna minicircle sequences (Borst and others 1987). Watanapokasin and others (1998) successfully used the rapd approach for discerning the intraspecific variation in T evansi in dairy cattle. Inter‐ and intraspecies variation of African try‐panosomes by rapd analysis has also been described (Waitumbi and Murphy 1993).…”

Section: Primer Sequence Length (Number Of Nucleotides) Guanine and Cmentioning

confidence: 99%

DNA polymorphism in Tiypanosoma evansi isolates defined by randomly amplified polymorphic DNA‐PCR

et al. 2001

View full text Add to dashboard Cite

show abstract

Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction

Cited by 11 publications

References 11 publications

Trypanosoma evansiand Surra: A Review and Perspectives on Origin, History, Distribution, Taxonomy, Morphology, Hosts, and Pathogenic Effects

Trypanosoma evansiand Surra: A Review and Perspectives on Origin, History, Distribution, Taxonomy, Morphology, Hosts, and Pathogenic Effects

Genetic Diversity of Trypanosoma evansi in Buffalo based on Internal Transcribed Spacer (ITS) Regions

DNA polymorphism in Tiypanosoma evansi isolates defined by randomly amplified polymorphic DNA‐PCR

Contact Info

Product

Resources

About