DNA polymorphism in <i>Tiypanosoma evansi</i> isolates defined by randomly amplified polymorphic DNA‐PCR

Omanwar, Swati; Singh, Raj Kumar; Butchaiah, G.; Rao, J. R.

doi:10.1136/vr.148.8.244

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Phylogenetic analysis of ITS sequences was used in [24] for the study of T. rangeli polymorphism. In T. brucei [25], T. congolense [26], and T. evansi, genetic variation in intra-species Trypanosoma has already been identified using PCR-based approaches. The goal of this study is to use internal transcribed spacer 1 primers (ITS-1) to investigate and distinguish the genetic relationship between isolates of T. evansi from camels in Riyadh and Al-Qassim, Saudi Arabia.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Metwally

Al-Turaiki

Altwaijry

et al. 2021

Animals

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We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

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Section: Introductionmentioning

confidence: 99%

Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Metwally

Al-Turaiki

Altwaijry

et al. 2021

Animals

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“…Previously, the intra-species genetic variation of Trypanosoma have been reported in T. brucei [2], T. congolense [30], and T. evansi [33,46] in different hosts basically revealed by PCR based method. Herein, we investigated the diversity of T. evansi in a single host (buffalo) using the nucleotide data of 18S rDNA, 5.8S rDNA, and ITS regions.…”

mentioning

confidence: 99%

Genetic Diversity of Trypanosoma evansi in Buffalo based on Internal Transcribed Spacer (ITS) Regions

Khuchareontaworn

Singhaphan

Viseshakul

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The nucleotide sequences of 18S rDNA and internal transcribed spacer (ITS) regions were used for studying the relationships of Trypanosoma evansi isolate from a buffalo. The sequences were analyzed and compared to 18S rDNA and the ITS regions of the other Trypanosoma spp. Maximum likelihood phylogenetic trees were constructed using Leishmania major as the outgroup. The tree of 18S rDNA indicated that T. evansi (buffalo B18) isolate was closely related to those of Taiwan and T. brucei stock. The ITS tree showed the genetic diversity among 32 clones of T. evansi (B18) within a single host. This data will be useful for epidemiological and dynamic studies for designing the rational control programs of the disease. KEY WORDS: internal transcribed spacers (ITS), phylogenetic, small subunit rDNA, Trypanosoma evansi.

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“…(Yu et al 1997), and Trypanosoma spp. (Basagoudanavar et al 1999;Omanwar et al 2001) is increasingly recognized. The present investigation on molecular characterization of F. gigantica isolates derived from cattle, buffalo, and goat was undertaken with the objective to study the variability, if any, between the bovine, bubaline, and caprine isolates of F. gigantica by RAPD analysis.…”

Section: Introductionmentioning

confidence: 93%

Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction

Gunasekar¹,

Tewari

Sreekumar

et al. 2008

Parasitol Res

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The present communication focuses on molecular characterization of Fasciola gigantica isolates derived from cattle, buffalo, and goat using random-amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis to elucidate genetic variability between the three isolates. Seventeen random oligonucleotide primers of 10-11 bases with GC content varying from 50-81.8% were used in the study. Depending upon the F. gigantica isolate-primer combination, one to five fragments in the range of 327-1,973 bp were amplified. It was significant to observe that, out of the 17 primers directing amplification of DNA fingerprints, only two designated as AP9 and AP14 were found to be of potential interest in the generation of polymorphic DNA. On the basis of similarity coefficient data, it may be suggested that cattle and buffalo isolates of F. gigantica show 100% homogeneity against 92.68% similarity coefficient observed between goat and cattle/buffalo isolates. In other words, 7.32% divergence was observed between goat and cattle/buffalo isolates while the primers AP1, AP4, AP10, AP13, and AP17 were able to generate monomorphic DNA fingerprints. Primers AP9 and AP14 are potentially informative in terms of the polymorphic nature of the fingerprints generated in RAPD assays. The finding of the absence of 626-bp DNA fragment in the goat and the uniqueness of the AP14 in generating a single RAPD-PCR product of 1,211 bp as against the product size of 1,162 bp in cattle/buffalo seem to be significant. This is the first report of elucidation of RAPD-PCR based molecular variability in the DNA fingerprinting pattern of F. gigantica isolated from cattle, buffalo, and goat.

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DNA polymorphism in Tiypanosoma evansi isolates defined by randomly amplified polymorphic DNA‐PCR

Cited by 7 publications

References 11 publications

Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Genetic Diversity of Trypanosoma evansi in Buffalo based on Internal Transcribed Spacer (ITS) Regions

Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction

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