Intra‐Articular Use of a Platelet‐Rich Product in Normal Horses: Clinical Signs and Cytologic Responses

Textor, Jamie A.; Tablin, Fern

doi:10.1111/j.1532-950x.2013.12015.x

Cited by 46 publications

(64 citation statements)

References 69 publications

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“…Consequently the intermittent significant difference between groups should be interpreted with caution. However, it cannot be excluded that PRP injection effectively induced a transient inflammatory response [29, 83] which might have contributed to a temporary decrease in echogenicity and in alignment of fibrous structures. This contrasts with results of an experimental equine study showing no differences at all for several B-mode parameters between SDFTs treated with a leucocyte poor single centrifugation plasma product and saline [84].…”

Section: Discussionmentioning

confidence: 99%

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

et al. 2016

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BackgroundRegenerative and anti-inflammatory effects on tendinopathies have been attributed to blood-derived biologicals. To date the evidence for the efficacy of autologous platelet-rich plasma (PRP) treatment of naturally occurring equine tendinopathies is limited. The purpose of this placebo-controlled clinical trial was to describe the effect of a single treatment of equine superficial digital flexor tendon (SDFT) disease with PRP on clinical and ultrasonographic parameters. Twenty horses with naturally occurring tendinopathies of forelimb SDFTs were randomly assigned to the PRP-treated group (n = 10) or control group (n = 10) after clinical and ultrasonographic examination. The SDFTs received an intralesional treatment with autologous PRP or were injected with saline, respectively (day 0). All horses participated in a standardized exercise programme and were re-examined clinically, with B-mode ultrasonography (5 times at regular intervals) and ultrasound tissue characterization (week 12 and 24 after treatment) until week 24. Long-term performance was estimated via telephone inquiry.ResultsCompared to day 0, lameness decreased significantly by week 8 after treatment with PRP and by week 12 in the control group. Ultrasonographically there was no difference in the summarized cross sectional area between the groups at any time point. Ultrasound tissue characterization showed that echo types representing disorganized matrix decreased significantly throughout the observation period in the PRP-treated group. Echo type II, representing discontinuous fascicles, not yet aligned into lines of stress was significantly higher 24 weeks after PRP treatment. Eighty percent of the PRP treated horses reached their previous or a higher level of performance after 12 months compared to 50 % in the CG. After 24 months these proportions were 60 % and 50 %, respectively.ConclusionsA single intralesional treatment with PRP up to 8 weeks after onset of clinical signs of tendinopathy contributes to an earlier reduction of lameness compared to saline treatment and to an advanced organization of repair tissue as the fibrillar matrix is getting organized into fascicles while remodelling continues. Long term, PRP treatment has the potential to increase the number of horses reaching their previous level of performance. Earlier treatment of tendinopathy with PRP should be considered to enhance these effects.

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Section: Discussionmentioning

confidence: 99%

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

et al. 2016

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“…Mesmo em contato com o colágeno no local inflamado, a concentração de fatores de crescimento liberada corresponde apenas a 10% do total plaquetário. Sendo assim, um adequado protocolo de ativação deve ser instituído, sendo a trombina bovina utilizada para a formação do coágulo de fibrina na obtenção do gel e o cloreto de cálcio para a ativação plaquetária, uma vez que o cloreto de cálcio se mostra mais eficiente quando comparado à outros métodos de ativação (Yamada 2011, Textor & Tablin 2012 A diferença observada na avaliação macroscópica e microscópica entre os grupos, indicando um tecido de reparação com superior produção de matriz extracelular e mais semelhante à cartilagem articular observado no GA foi condizente com os relatos da literatura atual (Yamada 2011, Xie et al 2012, Textor & Tablin 2013, Lee et al 2013. O PRP é eficaz na reparação da cartilagem, promovendo a proliferação celular, principalmente de condróci-tos, e consequentemente estimulando a síntese de matriz extracelular, como proteoglicanos e colágeno tipo II (Krü-ger et al 2012, Tsuzuki et al 2013.…”

Section: Discussionunclassified

Arcabouço de PRP-gel associado a células tronco mesenquimais: uso em lesões condrais em modelo experimental equino

et al. 2016

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Resumo: O plasma rico em plaquetas (PRP) é conhecido por apresentar propriedades anabólicas, anti-inflamatórias e capacidade de gelificação. Atualmente o PRP é considerado eficaz na reparação da cartilagem, sendo sua capacidade de formação de gel indicada para o preenchimento de defeitos condrais. O objetivo desse estudo foi analisar o uso do PRP ativado, no formato de arcabouço, como suporte para o implante de células tronco mesenquimais (CTM), no preenchimento e tratamento de lesões condrais induzidas em equinos. Doze equinos foram submetidos a uma cirurgia artroscópica no tempo zero do experimento (T0), onde foi induzida uma lesão condral de 15 mm de diâmetro na tróclea medial femoral dos membros pélvicos direito. As 12 articulações foram divididas em dois grupos distintos com seis articulações cada (GA e GB). As articulações do GA foram submetidas ao tratamento com o implante de CTM em gel de PRP. As articulações de GB foram o grupo controle do experimento. As CTMs foram extraídas do tecido adiposo e o PRP em gel foi obtido por protocolo de dupla centrifugação seguido da adição de trombina liofilizada. Após cinco meses (T150) foi realizada nova artroscopia para avaliação macroscópica do local, coleta de amostras do tecido de reparação para análises de microscopia eletrônica, sendo realizadas imagens ressonância magnética e tomografia computadorizada no local do implante no GA. Observamos que o gel de PRP associado às CTM demonstrou ser adequado no tratamento de defeitos condrais experimentais dos equinos. GA evidenciou um melhor aspecto macroscópico e microscópico do tecido de reparação, sendo que GB mostrou maior desorganização das fibras colágenas. Nas imagens de ressonância magnética e tomografia computadorizada apenas foi relevante o local da lesão condral. O arcabouço de gel de PRP demonstrou ser apropriado no suporte do tratamento com as CTMs, sendo de fácil aplicação e efetivo, demonstrando resultados promissores na reparação de lesões condrais induzidas.

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“…To date, there are no documented studies investigating the use of PRP in the joints of horses with clinical disease. Recently, various methods of PRP activation followed by IA fetlock joint administration were evaluated in normal horses using one commercial product (E-PET System, Leicestershire, UK) (94). This study was focused on evaluating activation systems in light of reported inflammatory responses occurring post-injection; however, so conclusions regarding PRP's role in cartilage resurfacing remain eagerly awaited.…”

Section: Platelet-rich Plasma (Prp)mentioning

confidence: 99%

Cartilage Therapy and Repair in Equine Athletes

Johnson

Frisbie

2016

Operative Techniques in Orthopaedics

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Intra‐Articular Use of a Platelet‐Rich Product in Normal Horses: Clinical Signs and Cytologic Responses

Abstract: Resting and CaCl2 -activated PRPr may be safely used to treat equine joints, but bovine thrombin activation is not recommended at 10 U/mL. A PRPr can be prepared using a gravity filtration system, eliminating the need for centrifugation.

Cited by 46 publications

References 69 publications

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies – a randomized prospective controlled clinical trial

Arcabouço de PRP-gel associado a células tronco mesenquimais: uso em lesões condrais em modelo experimental equino

Cartilage Therapy and Repair in Equine Athletes

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