Intra-articular injection of hydrogen sulfide decreased the progression of gonarthrosis

Abstract

Hydrogen sulfide (H2S) is found in both the plasma and synovial fluid of patients with gonarthrosis. In the present study, we investigated whether intra-articular injection of sodium hydrosulfide (NaSH) (1 mM, 30 μL), a H2S donor, might affect gonarthrosis in rats. Gonarthrosis was induced surgically in the left knees of rats and left for 6 weeks for the development of disease. Then, intra-articular injections of NaSH or methylprednisolone (1 mg/kg, 30 μL) were administered to rats. Half of each group was sacrificed at the end of the first day and the other half was sacrificed at the end of 4 weeks to evaluate early and later effects of injections on gonarthrosis. The injury induced by anterior cruciate ligament resection and medial meniscectomy in rats caused the development of gonarthrosis. As the duration lengthened after gonarthrosis induction, the progression of the disease continued. According to the modified Mankin Scoring System, intra-articular injection of NaSH histopathologically slowed the progression of gonarthrosis, whereas methylprednisolone was ineffective. In addition, NaSH decreased apoptosis in rat knees with gonarthrosis. Each treatment did not cause injury to healthy knees. Our results lead to the consideration that intra-articular NaSH administration may be effective in the progression of gonarthrosis.

Intraarticular Administration Effect of Hydrogen Sulfide on an In Vivo Rat Model of Osteoarthritis

“…We observed that intraarticular GYY-4137 administration protected cartilage against deterioration induced by the OA model. Previous studies support these findings [ 26 , 28 , 34 ]. For instance, the intra-articular injection of sodium hydrosulfide (NaSH), a fast-releasing H 2 S donor, slowed the development of degenerative changes in the articular cartilage in a model of gonarthrosis [ 26 ].…”

“…Previous studies support these findings [ 26 , 28 , 34 ]. For instance, the intra-articular injection of sodium hydrosulfide (NaSH), a fast-releasing H 2 S donor, slowed the development of degenerative changes in the articular cartilage in a model of gonarthrosis [ 26 ]. Likewise, cartilage damage as well as proteoglycan loss were exacerbated in 3-MST KO joints compared to WT joints in an experimental OA in mice [ 34 ].…”

“…Additionally, other authors observed in vitro similar actions of the gas in the chondrocyte, such as attenuating inflammatory signaling and exerting chondroprotective effects [ 21 , 22 , 25 ]. By in vivo experimental approaches, different studies have also detected the beneficial effect of the administration of H 2 S donors on rheumatic disease progression and pain [ 26 , 27 ]. Interestingly, we have recently shown that balneotherapy in sulfur-rich water attenuates the destruction of cartilage and pain levels in an OA model in rats by the surgical destabilization of the joint [ 28 ].…”

The Recovery of Cognitive and Affective Deficiencies Linked with Chronic Osteoarthritis Pain and Implicated Pathways by Slow-Releasing Hydrogen Sulfide Treatment

“…Numerous findings have demonstrated the beneficial anti-inflammatory and protective actions induced by the exogenous administration of H 2 S in the cartilage and chondrocytes in cell cultures [32,33]. Other works have also proven the positive effects of the intra-articular administration of H 2 S donors on rheumatic disease progression [34], cartilage destruction, and oxidative damage in rats [35]. The antinociceptive and antidepressant effects of the systemic administration of H 2 S donors, such as isothiocyanates, in mice with knee osteoarthritis have also been shown [2,36].…”

Long non‑coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3

“…All operations were carried out under sterile conditions in the specific pathogen-free animal center. Firstly, the animals were randomly assigned into the following six groups to examine the efficiency of transfection (n=3): i) The control group [injection of the same volume of the vehicle control (PBS) into knee joints] ( 19 , 20 ); ii) shRNA-NC group (injection of shRNA-NC into knee joints); iii) shRNA-XIST-1 group (injection of shRNA-XIST-1 into knee joints); iv) shRNA-XIST-2 group (injection of shRNA-XIST-2 into knee joints); v) OverExp-NC group (injection of overexpression-NC plasmids into knee joints); and vi) OverExp-XIST group (injection of overexpression-XIST plasmids into knee joints). Subsequently, the animals (different rats) were randomly assigned into the following five groups using simple randomization (n=12): i) The control group [sham operation + injection of the same volume of vehicle control (PBS) into knee joints]; ii) overexpression (OverExp)-XIST group control (sham operation + injection of overexpression-XIST plasmids into knee joints); iii) model group (OVX + injection of the same volume of control vehicle (PBS) into knee joints); iv) model + short hairpin (sh)RNA-negative control (NC) group (OVX + injection of shRNA-NC into knee joints); and v) model + shRNA-XIST group (OVX + injection of shRNA-XIST into knee joints).…”