Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats

Yeh, Tsu-Te; Wen, Zhi Hong; Lee, Herng Sheng; Lee, Chian Her; Yang, Zhi; Jean, Yen-Hsuan; Wu, Shing‐Sheng; Nimni, Marcel E.; Han, Bo

doi:10.1007/s00586-008-0594-0

Cited by 45 publications

(64 citation statements)

References 24 publications

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“…The histological findings of severe cartilage degeneration at 28 days compare favorably to those of Tachihara et al [46], who used injection of Complete Freund's Adjuvant, Kim et al [51]—who used monosodium iodoacetate (MIA) and to those of Yeh et al [57], who injected collagenase into a single lumbar facet joint. …”

Section: Discussionsupporting

confidence: 58%

Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model

Henry

Yashpal

Vernon

et al. 2012

Pain Research and Treatment

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Objective. To develop a novel animal model of persisting lumbar facet joint pain. Methods. Sprague Dawley rats were anaesthetized and the right lumbar (L5/L6) facet joint was exposed and compressed to ~1 mm with modified clamps applied for three minutes; sham-operated and naïve animals were used as control groups. After five days, animals were tested for hind-paw sensitivity using von Frey filaments and axial deep tissue sensitivity by algometer on assigned days up to 28 days. Animals were sacrificed at selected times for histological and biochemical analysis. Results. Histological sections revealed site-specific loss of cartilage in model animals only. Tactile hypersensitivity was observed for the ipsi- and contralateral paws lasting 28 days. The threshold at which deep tissue pressure just elicited vocalization was obtained at three lumbar levels; sensitivity at L1 > L3/4 > L6. Biochemical analyses revealed increases in proinflammatory cytokines, especially TNF-α, IL-1α, and IL-1β. Conclusions. These data suggest that compression of a facet joint induces a novel model of local cartilage loss accompanied by increased sensitivity to mechanical stimuli and by increases in inflammatory mediators. This new model may be useful for studies on mechanisms and treatment of lumbar facet joint pain and osteoarthritis.

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Section: Discussionsupporting

confidence: 58%

Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model

Henry

Yashpal

Vernon

et al. 2012

Pain Research and Treatment

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“…injection of collagenase is an established model of OA that has been predominantly used to study the mechanisms underlying structural joint damage [15-17]. Histopathological alterations of the knee joint very similar to those observed in human OA have been described for this model, particularly in mice [18,19] and rabbits [20], as well as in the lumbar facet joint of rats [21]. …”

Section: Introductionmentioning

confidence: 99%

Intra-articular injection of collagenase in the knee of rats as an alternative model to study nociception associated with osteoarthritis

et al. 2014

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IntroductionAnimal models currently used in osteoarthritis-associated pain research inadequately reproduce the initiating events and structural pathology of human osteoarthritis. Conversely, intra-articular injection of collagenase is a structurally relevant model, as it induces articular degeneration both by digesting collagen from cartilage and by causing articular instability, thereby reproducing some of the main events associated with osteoarthritis onset and development. Here, we evaluated if the intra-articular injection of collagenase can be an alternative model to study nociception associated with osteoarthritis.MethodsOsteoarthritis was induced by two intra-articular injections of either 250 U or 500 U of collagenase into the left knee joint of adult male Wistar rats. A six weeks time-course assessment of movement- and loading-induced nociception was performed by the Knee-Bend and CatWalk tests. The effect of morphine, lidocaine and diclofenac on nociceptive behaviour was evaluated in animals injected with 500 U of collagenase. Joint histopathology was scored for both doses throughout time. The expression of transient receptor potential vanilloid 1 (TRPV1) in ipsilateral dorsal root ganglia (DRG) was evaluated.ResultsAn increase in nociceptive behaviour associated with movement and loading of affected joints was observed after intra-articular collagenase injection. With the 500 U dose of collagenase, there was a significant correlation between the behavioural and the histopathological osteoarthritis-like structural changes developed after six weeks. One week after injection of 500 U collagenase, swelling of the injected knee and inflammation of the synovial membrane were also observed, indicating the occurrence of an early inflammatory reaction. Behavioural changes induced by the 500 U dose of collagenase were overall effectively reversed by morphine and lidocaine. Diclofenac was effective one week after injection. TRPV1 expression increased six weeks after 500 U collagenase injection. ConclusionWe conclude that the intra-articular injection of 500 U collagenase in the knee of rats can be an alternative model for the study of nociception associated with osteoarthritis, since it induces significant nociceptive alterations associated with relevant osteoarthritis-like joint structural changes.

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“…34 After that, destruction of the extracellular matrix with subchondral bone exposure, subchondral bone sclerosis, and osteophyte formation are seen. 35 Clinical symptoms of OA include pain, inflammation, and a limited range of motion. 36 Administration of the VT-SR at 500 mg/kg for 28 days significantly ameliorated type II collagenase-induced inflammation and pain levels in the rat knee (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The calibration equations and correlation coefficients of resveratrol, hopeaphenol, and (+)-e-viniferin revealed linear relationships between the peak areas and concentrations ( Table 2). The respective retention times and contents of resveratrol, hopeaphenol, and (+)-e-viniferin in VT-SR were 30.0 min (1.1 mg/g), 35.1 min (6.7 mg/g), and 40.9 min (5.1 mg/g).…”

Section: Phytochemical Contents Of the Vt-lb And Vt-srmentioning

confidence: 99%

Anti-Inflammatory Effects of Vitis thunbergii var. taiwaniana on Knee Damage Associated with Arthritis

Tsai

Wang

Chen

et al. 2014

Journal of Medicinal Food

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Vitis thunbergii Sieb. et Zucc. var. taiwaniana Lu (VT) is an indigenous plant in Taiwan that is traditionally used for promoting joint health. In this study, we used in vitro primary human chondrocytes (PHCs) and two in vivo animal models to evaluate the anti-inflammatory effects of VT on arthritis. Results showed that the water extract of the stems and roots from VT (VT-SR) was rich in flavones and phenols with 1.1 mg/g of resveratrol, 6.7 mg/g of hopeaphenol, and 5.1 mg/g of (+)-e-viniferin. VT-SR significantly scavenged DPPH radicals and inhibited prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-induced PHCs without exhibiting significant cytotoxicity. In in vivo models, the VT-SR (500 mg/kg) significantly decreased serum PGE2 and knee 2-18 F-fluoro-2-deoxy-D-glucose ( 18 F-FDG) levels in LPS-induced acute inflammatory arthritis in rabbits. In addition, dietary supplementation with VT-SR for 28 days significantly alleviated type II collagenase-induced rat osteoarthritis with improvements in weight bearing and range of motion tests. In conclusion, our results suggest that the VT-SR is a good candidate for developing dietary supplements to prevent joint deterioration and inhibit inflammation.

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Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats

Cited by 45 publications

References 24 publications

Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model

Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model

Intra-articular injection of collagenase in the knee of rats as an alternative model to study nociception associated with osteoarthritis

Anti-Inflammatory Effects of Vitis thunbergii var. taiwaniana on Knee Damage Associated with Arthritis

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