Intra-arterial administration of tumor-targetingSalmonella typhimuriumA1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model

Abstract

Previously, a patient-derived orthotopic xenograft (PDOX) model was established with a lung metastasis from an osteosarcoma patient which developed after adjuvant cisplatinum (CDDP) treatment. In this model, we previously demonstrated the efficacy of trabectedin (TRAB) and temozolomide (TEM) compared with CDDP. In the present report, osteosarcoma tissue was implanted orthotopically in the distal femur of mice which were randomized into the following groups when tumor volume reached approximately 100 mm 3 ; On day 14 after initiation of treatment, all but CDDP significantly inhibited tumor volume growth compared with untreated controls. Control (G1

The contribution of immune infiltrates and the local microenvironment in the pathogenesis of osteosarcoma

“…Several models of PDX of osteosarcoma has been described in the literature for various assessment including epigenomic, drug testing and therapeutic development [122][123][124][125][126]. These immunodeficient were very recently used for analysing the tumor-targeting amino-acidauxotrophic strain Salmonella typhimurium A1-R [125,126]. Salmonella typhimurium A1-R exhibit a marked potential for infecting cancer cells and inducing cell death.…”

“…Salmonella typhimurium A1-R exhibit a marked potential for infecting cancer cells and inducing cell death. More interestingly, bacteria induced the regression of cisplatin-resistant relapsed tumors [126].…”

Metastatic tumor cells detection and anti‐metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma

“…In order to achieve this purpose, the patient‐derived orthotopic xenograft (PDOX) mouse models for osteosarcoma has been established in some laboratories . Although the perceived technical complexity and the appropriate quality control needed to optimize the viability of the original human tumor cells, such PDOX models can play an important role for individualized therapy approaches in translational research as they are more closely recapitulate the heterogeneity of primary human osteosarcoma . In our study, we showed suppression of spontaneous metastasis to the lungs with the repeated systemic administrations of rVSV‐K in a syngeneic ectopic murine LM8 osteosarcoma mouse model.…”

“…It has been recently observed that a large genomic heterogeneity exists between osteosarcoma patients so that individualized precision therapy is needed in the management of osteosarcoma . In order to achieve this purpose, the patient‐derived orthotopic xenograft (PDOX) mouse models for osteosarcoma has been established in some laboratories . Although the perceived technical complexity and the appropriate quality control needed to optimize the viability of the original human tumor cells, such PDOX models can play an important role for individualized therapy approaches in translational research as they are more closely recapitulate the heterogeneity of primary human osteosarcoma .…”

Visualizing the Tumor Microenvironment by Color-coded Imaging in Orthotopic Mouse Models of Cancer

“…Recently, PDOX models of sarcoma (47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64), cervical cancer (65-67) as well as melanoma (68)(69)(70)(71)(72)(73)(74) have also been developed.…”