Intra- and Intergeneric Relationships of the Genus Aquaspirillum: Prolinoborus, a New Genus for Aquaspirillum fasciculus, with the Species Prolinoborus fasciculus comb. nov.

Phylogenetic relationships among members of the Comamonadaceae, and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov.

“…Several species studied are assigned to incorrect genera and these combinations are indicated by enclosing the genus name in square brackets. The [Aquaspirillum] species were grown on PSS agar (Pot et al, 1992b), Xylophilus ampelinus was grown on GYCA medium (Willems et al, 1987), and all other species were grown on medium B of King et al (1954). Xylophilus ampelinus was incubated at 24 "C, [Aquaspirillum] psychrophilum was grown at 20 "C, and all other species were grown at 28 "C, for 48-72 h. PCR amplification of 165 rRNA genes.…”

“…Current knowledge of the phylogenetic relationship of the members of the Comamonadaceae is based on 00868 0 1999 IUMS rRNA cistron similarities (Pot et al, 1992b;Willems et al, 1991aWillems et al, , b, 1992b, which was the most valid and useful parameter for drawing taxonomic conclusions at the generic and suprageneric levels before 16s rRNA sequence analysis was available (Tamaoka et al, 1987). Based on the DNA-rRNA hybridization results (Pot et al, 1992b;Willems et al, 1991aWillems et al, , b, 1992b, sixteen rRNA branches within the Comamonadaceae were delineated : five Cornamonas rRNA branches, one Hydrogenophaga rRNA branch, one Xylophilus rRNA branch, one Variovorax rRNA branch, two Acidovorax rRNA branches and six [Aquaspirillum] rRNA branches. Willems et al (1987) and Vandamme et al (1996) concluded that each of the sub-branches in the acidovorans rRNA complex (i.e.…”

Proposals of Curvibacter gracilis gen. nov., sp. nov. and Herbaspirillum putei sp. nov. for bacterial strains isolated from well water and reclassification of [Pseudomonas] huttiensis, [Pseudomonas] lanceolata, [Aquaspirillum] delicatum and [Aquaspirillum] autotrophicum as Herbaspirillum huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov. and Herbaspirillum autotrophicum comb. nov.

“…However, DNA-rRNA hybridization, chemotaxonomic analysis and 16S rRNA gene sequence studies (Pot et al, 1992;Hamana et al, 1994;Wen et al, 1999;Ding & Yokota, 2002) have shown that the genus is taxonomically heterogeneous. Among the species of this genus, some are closely related to species of the genera Herbaspirillum, Magnetospirillum and Pseudomonas.…”

Isolation and chemical characterization of lipopolysaccharides from four Aquaspirillum species (A. itersonii subsp. nipponicum IFO 13615, A. polymorphum IFO 13961, A. aquaticum IFO 14918, A. metamorphum IFO 13960 and A. metamorphum mutant strain 12-3).

“…The name Aquaspirillum aquaticum will not anymore be used. Aquaspirillum aquaticum has recently been renamed as Comamonas terrigena and forms together with Comamonas acidovorans and Comamonas testosteroni the genus Comamonas, which represents the Acidovorans-Cluster or /3-1 group of Proteobacteria (21,29,30,32 The present investigation will provide further arguments based on LPS composition for the classification of four Aquaspirillum strains in two different subdivisions of the Proteobacteria.…”