Intra-albumin migration of bound fatty acid probed by spin label ESR

Gurachevsky, Andrey; Shimanovitch, Ekaterina; Gurachevskaya, Tatjana; Muravsky, Vladimir

doi:10.1016/j.bbrc.2007.06.140

Cited by 16 publications

(23 citation statements)

References 10 publications

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“…Albumin as the major transport protein in the human organism protects these cancer markers from catabolism and significantly amplifies their concentration. It is confirmed [3], [4], [5] that these proteins block the albumin function by changing its conformation. The conformational change of serum albumin, and thus its transport and detoxification properties, can be determined with the help of the ESR spectrum.…”

Section: Introductionmentioning

confidence: 52%

“…The conformational change of serum albumin, and thus its transport and detoxification properties, can be determined with the help of the ESR spectrum. We introduced in previous work the MMS-Test (mobility of molecular structure) and the corresponding feature extraction, the analysis of which is not the objective of this paper [2], [5], [4], [6], [7]. The proprietary MMS-Test offers the opportunity to detect cancer independent of its type and location by determining albumin binding, transport and structural flexibility parameters.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expectation-maximization-estimation of mixture densities for Electron-Spin-Resonance-analysis of albumin

Schmidt¹,

Krumbiegel

Waterstradt³

et al. 2009

2009 IEEE International Workshop on Genomic Signal Processing and Statistics

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Early diagnosis of human cancer is of crucial importance for successful therapies. Cancer diagnosis via ESR (Electron-Spin-Resonance) spectroscopy of albumin found in human blood provides a new promising approach. The ESR frontend signal processing follows a protocol of our proprietary 'mobility of molecular structure test' (MMSTest) and provides a real-valued 33-dimensional vector representation per sample, which combines a representative feature set of the binding ability (spin-probes) of albumin under investigation. Classical statistical pattern recognition is then applied to the feature vector, including LDA and EM mixture density estimation, leading to a classification error rate of 14% between the two patient classes 'healthy' and 'suspect'. The class 'suspect' includes cancer and other chronic condition. The investigation was performed on a proprietary database of MedInnovation with 1176 cancer and non-cancer patients.

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Section: Introductionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Expectation-maximization-estimation of mixture densities for Electron-Spin-Resonance-analysis of albumin

Schmidt¹,

Krumbiegel

Waterstradt³

et al. 2009

2009 IEEE International Workshop on Genomic Signal Processing and Statistics

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“…Ethanol, extra pure, (Merck Millipore) was used for modifying the binding affinity of the fatty acid spin probe to albumin. The final concentrations of ethanol (mol/l) and spin probe (10 -3 mol/l) were 2.9 and 0.83 in aliquot 1, 3.4 and 1.61 in aliquot 2, and 3.8 and 2.34 in aliquot 3, respectively (16).…”

Section: Methodsmentioning

confidence: 99%

Conformational changes in inter-α-trypsin inhibitor heavy chain 4 activate its tumor-specific activity in mice with B16 melanoma

Kormosh¹,

Давидова²,

Kopyl'tsov³

et al. 2015

Molecular Medicine Reports

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It is known that blood serum proteins of tumor‑bearing mice display tumor‑specific activity. However, to date, the nature of this activity has remained elusive, and no tumor‑specific proteins have been detected in the blood serum of tumor‑bearing animals compared with those in healthy animals. The present study postulated and investigated the hypothesis that the observed tumor‑specific activity of the blood serum proteins is not associated with the appearance of novel serum proteins but with changes in the conformation of the existing ones. The present study showed conformational changes of two serum albumin proteins and inter‑α‑trypsin inhibitor heavy chain 4 (ITIH4) in mice with B16 melanoma compared to tumor‑free mice, as determined by differences in the products of proteolysis by proteomic analysis following column chromatography. The differences in the conformation of serum albumin in mice with B16 melanoma and tumor‑free mice were accompanied by a change in the interaction of these molecules with the fatty acid spin probe 16‑doxyl stearic acid. The differential conformation of ITIH4 in mice with B16 melanoma and that in tumor‑free mice was accompanied by inhibition of tumor growth and increased life span. Analysis of the role of protease‑anti‑proteases (serpins) in the serum of tumor‑bearing animals in tumor growth confirmed the hypothesis that tumor growth in the body is mediated, at least in part, via balancing of serpins.

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“…Ratio spin-probe/protein equals 2 даже использован для оценки времени корреляции вращательной диффузии молекулы белка и оценки индекса гидрофобности окружения нитроксильной группы зонда [Кузнецов, 1976]. При этом ЖК способны обмениваться центрами сорбции на белке [Gurachevsky et al, 2007], что также может вносить вклад в возникновение В-компоненты спектра.…”

Section: материалы и методыunclassified

“…Также было обнаружено, что глобальное разворачивание структуры альбумина, вызванное связыванием ЖК, увеличивает гибкость микроокружения Цис-34 [Pavićević et al, 2017], что сопряжено с его способностью к окислению [Wetzel et al, 1980]. Связанные ЖК осуществляют перманентную миграцию между центрами связывания и доменами белка [Gurachevsky et al, 2007;Muravsky et al, 2009;Junk et al, 2011].…”

Section: Introductionunclassified

Conformational effects of interaction of serum albumine with nanoparticles of carbon shungyte: epr spin-probe study

Rozhkov

Горюнов

Goryunov

2018

Proceedings of KarRC of RAS

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Intra-albumin migration of bound fatty acid probed by spin label ESR

Cited by 16 publications

References 10 publications

Expectation-maximization-estimation of mixture densities for Electron-Spin-Resonance-analysis of albumin

Expectation-maximization-estimation of mixture densities for Electron-Spin-Resonance-analysis of albumin

Conformational changes in inter-α-trypsin inhibitor heavy chain 4 activate its tumor-specific activity in mice with B16 melanoma

Conformational effects of interaction of serum albumine with nanoparticles of carbon shungyte: epr spin-probe study

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