Intestinal mucosal microdialysis: Histamine release in splanchnic ischemia/reperfusion injury in piglets

Reperfusion but Not Acute Ischemia in Pig Small Intestine Induces Transcriptionally Mediated Heat Shock Response in situ

“…Interestingly, lactate concentration continued to increase at the beginning of reperfusion, presumably indicating continuing mucosal dysfunction. On the other hand, complete reperfusion was ascertained by decreased lactate and dpCO 2 , which is in accordance with our previous studies [20,[25][26]. In our model, the control biopsies taken after the stabilization period and incubated ex vivo showed that surgical procedures or the experimental setting did not influence the heat shock response.…”

Intraperitoneal microdialysis for detection of splanchnic metabolic disorders

“…Several studies have confirmed microdialysis to be a valuable tool for assessing the metabolic state of organs during critical conditions [18,19,20,21]. To our knowledge, no data are available on the intraperitoneal microdialysis of the ascites as a reflection of biochemical disorders in the mesenteric region (e.g., ischemia, hypoxia).…”

Use of Rapid Sampling Microdialysis for Intraoperative Monitoring of Bowel Ischemia

“…Microdialysis was developed in the early seventies to sample the extracellular fluid from brain tissue 8 and it has also been used to monitor ischemia in a range of human tissues including brain, 9 liver, 10 heart, 11 muscle, and fat. 12 To date, microdialysis has not been used for intramural monitoring of human bowel; only used in animal studies [13][14][15] and intraperitoneal (extramural) monitoring in humans. 16 Most microdialysis techniques are conducted offline, involving serial manual sampling and analysis, which is laborious, time consuming, and can lead to a delay in the detection of ischemic events.…”