Intraperitoneal microdialysis for detection of splanchnic metabolic disorders

Klaus, Stephan; Heringlake, Matthias; Gliemroth, Jan; Bruch, Hans–Peter; Bahlmann, Ludger

doi:10.1007/s00423-002-0320-z

Cited by 30 publications

(21 citation statements)

References 18 publications

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“…Several intraperitoneal microdialysis studies of rats have been reported, but all were conducted by the same group (2,10,11,12). However, the technique, and in particular the introduction and positioning of microdialysis probes in the intraperitoneal fluid of anesthetized rats, could also be reproduced without any particular difficulty.…”

Section: Discussionmentioning

confidence: 99%

“…Microdialysis is a reliable technique for investigating the kinetics of antibiotics in different tissues, and it has been used on many occasions in various tissues, such muscle, lung, brain, and skin, in both animals and humans (18). However, no microdialysis antibiotic-distribution study has ever been conducted in intraperitoneal fluid to our knowledge, although the technique has been used to monitor intraperitoneal concentrations of markers of ischemia, such as lactate, mainly in pigs (13,22,24), but also in humans (8,19) and rats (2,10,11,12).…”

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confidence: 99%

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Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Lefeuvre

Marchand

Lamarche

et al. 2006

Antimicrob Agents Chemother

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The purpose of this study was to extend the use of microdialysis to the investigation of antibiotic distribution into the intraperitoneal fluid of rats with or without peritonitis. Microdialysis probes were inserted into the jugular vein and peritoneal cavity of control rats or rats with intra-abdominal sepsis (n ‫؍‬ 8 in each group) induced by cecal ligation and punctures. Imipenem (IPM) probe recoveries were determined in each rat by retrodialysis by drug. IPM was infused intravenously at a dose of 30 mg · kg ؊1 over 30 min, microdialysis samples were collected for 120 min, and IPM concentrations were determined by high-performance liquid chromatography. Intraperitoneal infection had no statistically significant effect on IPM clearance (11.9 ؎ 2.3 ml · min ؊1 · kg ؊1 in control rats versus 10.9 ؎ 2.1 ml · min ؊1 · kg ؊1 in rats with peritonitis) or the volume of distribution (296 ؎ 47 ml · kg ؊1 in control rats versus 310 ؎ 49 ml · kg ؊1 in rats with peritonitis). IPM concentration profiles in intraperitoneal fluid and blood were virtually superimposed in control rats, whereas in infected animals, the mean intraperitoneal IPM concentrations were apparently slightly lower than corresponding blood levels. However, the areas under the concentration-versus-time curve estimated in intraperitoneal fluid and blood were not significantly different in both groups, with the corresponding ratios close to unity (1.01 ؎ 0.19 and 0.89 ؎ 0.28 in control rats and rats with peritonitis, respectively). In conclusion, IPM distribution in intraperitoneal fluid is rapid and complete both in control rats and in rats with peritonitis.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Lefeuvre

Marchand

Lamarche

et al. 2006

Antimicrob Agents Chemother

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“…So far, the principle has been evaluated for intraperitoneal use. In animal studies and a few human studies, intraperitoneal microdialysis has been able to detect anastomotic bowel leakage before the development of signifi cant clinical symptoms [8][9][10][11][12][13][14][15]. The lactate/pyruvate ratio (L/P ratio) is the parameter with the highest sensitivity [15,16].…”

Section: Introductionmentioning

confidence: 99%

Mediastinal microdialysis: early diagnosis of anastomotic leakage after resection for esophageal cancer

et al. 2009

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The aim of the present study was to evaluate the safety of mediastinal microdialysis and its effi cacy regarding the early diagnosis of anastomotic leakage after gastroesophageal resection for esophageal cancer. Eight consecutive patients were included; one patient was excluded for reasons of catheter malfunction. The tip of the mediastinal microdialysis catheter was placed close to the anastomosis and held by a 4-0 absorbable suture. A subcutaneous microdialysis catheter placed in the pectoral region served as a reference. Samples collected every 4 h in the fi rst 8 postoperative days were analyzed for lactate, glucose, pyruvate, and glycerol and the lactate/pyruvate ratio (L/P ratio) was calculated. There were no procedure-related complications. Six patients had an uncomplicated postoperative course. In one patient, the L/P ratio was within normal range during the fi rst 20 h postoperatively, but a steady and signifi cant increase in L/P ratio then occurred, reaching a maximum of 105 after 124 h. The patient developed leakage symptoms on day 3, but endoscopy was unable to demonstrate any leakage. On the sixth postoperative day, blue dye administered through the nasogastric tube was recovered in the pleural drain. The leakage was treated with a covered selfexpanding metal stent; an immediate and signifi cant drop in the L/P ratio occurred. Mediastinal microdialysis seems to be a safe and promising tool in the early diagnosis of anastomotic leakage in patients undergoing gastroesophageal resection for cancer.

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“…However, intraluminal metabolites measurements have been found to be extremely low and the detection is not feasible due to the resting phase of the intestine and to the high volume of contents in the lumen (126). A common approach is to place the microdialysis probe in the human peritoneal cavity (127)(128)(129). The assumption is that due to the high amount of intestinal anaerobic products metabolised by the liver, the metabolic markers of an impaired circulation are greater in the peritoneum than in blood (77).…”

Section: Intestinal Microdialysismentioning

confidence: 99%

“…Several studies have used in vivo microdialysis monitoring in animal models to investigate splanchnic metabolic disorders by measuring glucose, lactate/pyruvate ratio and glycerol both intraluminally (132)(133)(134) and in the intraperitoneal space (128). From these studies, microdialysis was suggested as a valuable tool for surgeons to detect early signs of mesenteric ischemia and the postoperative complications associated with this.…”

Section: Off-line Microdialysismentioning

confidence: 99%

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

Priya¹,

Córcoles²

2012

Sepsis - An Ongoing and Significant Challenge

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Intraperitoneal microdialysis for detection of splanchnic metabolic disorders

Cited by 30 publications

References 18 publications

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

Mediastinal microdialysis: early diagnosis of anastomotic leakage after resection for esophageal cancer

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

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