Intestinal morphology, immunity and microbiota response to dietary fibers in largemouth bass, Micropterus salmoide

Dietary fibre (DF) is defined as 'non-digestible carbohydrates plus lignin' and includes non-starch polysaccharides (NSPs), hydrocolloids, resistant starch, resistant oligosaccharides and lignin associated with DF polysaccharides (Stephen et al., 2017). DF is not digested and absorbed by monogastric animals (Sinha et al., 2011), but can be fermented by intestinal microbiota (Miao et al., 2018).Therefore, it can regulate the structure of intestinal microbiota and improve intestinal health (Lin et al., 2020). In addition, DF has bile acid (BA) binding capacity Naumann et al., 2019) and reduces its reabsorption (Moriceau et al., 2000).BAs are synthesized from cholesterol. Therefore, appropriate DF intake can decrease cholesterol (Chen et al., 2018) and hepatic steatosis (Brockman et al., 2014). However, the effect of excessive DF intake on fish, which occurs with an increasing number of plant ingredients being included in the diet, has not received enough attention.

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mentioning

confidence: 99%

“…Therefore, it can regulate the structure of intestinal microbiota and improve intestinal health (Lin et al, 2020). In addition, DF has bile acid (BA) binding capacity Naumann et al, 2019) and reduces its reabsorption (Moriceau et al, 2000).…”

mentioning

confidence: 99%

Pectin and soybean meal induce stronger inflammatory responses and dysregulation of bile acid (BA) homeostasis than cellulose and cottonseed meal, respectively, in largemouth bass ( Micropterus salmoides ), which might be attributed to their BA binding capacity

Cai

Ren

et al. 2021

Aquaculture Research

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“…Gut microbiota is variable and shifty with nutrient supplies and water environment (such as temperature, pH and so on) (Giatsis et al, 2015;Maslowski et al, 2011). Fusobacteria, Proteobacteria and Firmicutes are the dominant bacterial phylum of largemouth bass intestine at 27.6 to 34℃ (Lin et al, 2020;Zhou et al, 2018). The present study showed that Proteobacteria, Actinobacteria and Firmicutes were the dominant bacterial phylum in largemouth bass at 20 ± 1°C.…”

Section: High-starch Diet Impaired Intestinal Microbial Barriermentioning

confidence: 50%

“…Total RNA samples isolated from the intestine using a BioFast Simply P Total RNA Extraction kit (Invitrogen, Carlsbad, CA, USA), then stored at -80 °C (Lin et al, 2020). The cDNA synthesized by using TianGen® FastKing RT Kit (With gDNase) and stored at -20 °C (Yu et al, 2018).…”

Section: Quantitative Real Time Pcr Analysismentioning

confidence: 99%

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Zhao¹,

Ji²,

Liao³

et al. 2021

Preprint

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The intestinal barrier is primarily composed of physical, physiological and microbial barriers, and intestine microbe-immune interactions influenced by diet in fish still remain increasingly unexplored. The intestinal barriers of Largemouth bass (Micropterus salmoides) (4.1±0.2g) were explored after they were fed three different starch diets for eight weeks (low starch,7%; middle starch, 12%; high starch, 17%). The results showed that high starch diet led slight infiltration of inflammatory cells and moderate loss of mucous membrane layer in midgut. Meanwhile, high starch diet decreased the antioxidant enzymes (T-SOD) activities and genes (SOD1, SOD2, SOD3a, CAT, GPX) expression significantly (P < 0.05). The MDA content was significantly increased with increasing starch level (P < 0.05). High starch diet up-regulated the expression of pro-inflammatory genes (IL-8, IL-1β and TNFα) and apoptosis genes (bax, caspase3, caspase8 and caspase9), whereas down-regulated the expression of anti-apoptosis genes bcl-2 and tight junction proteins genes (occludin and claudin7). In addition, the high starch diet increased the relative abundance of Actinobacteria and Firmicutes, resulted in microbial dysbiosis. In conclusion, high dietary starch impaired intestinal barriers, and increased the risk of disease outbreaks.

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“…Dietary fiber supplementation in animals can reduce intestinal inflammation, promoting intestinal antioxidant capacity and intestinal barrier function, etc. [11,12]. Some reports have proved that maternal fiber intake can significantly affect the development of offspring.…”

Section: Introductionmentioning

confidence: 99%

Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model

Xie

Liu

et al. 2020

Nutrients

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Dietary fiber intake during pregnancy may improve offspring intestinal development. The aim of this study was to evaluate the effect of maternal high fiber intake during late gestation on intestinal morphology, microbiota, and intestinal proteome of newborn piglets. Sixteen sows were randomly allocated into two groups receiving the control diet (CD) and high-fiber diet (HFD) from day 90 of gestation to farrowing. Newborn piglets were selected from each litter, named as CON and Fiber group, respectively. Maternal high fiber intake did not markedly improve the birth weight, but increased the body length, the ileal crypt depth and colonic acetate level. In addition, maternal high fiber intake increased the -diversity indices (Observed species, Simpson, and ACE), and the abundance of Acidobacteria and Bacteroidetes at phylum level, significantly increased the abundance of Bradyrhizobium and Phyllobacterium at genus level in the colon of newborn piglets. Moreover, maternal high fiber intake markedly altered the ileal proteome, increasing the abundances of proteins associated with oxidative status, energy metabolism, and immune and inflammatory responses, and decreasing abundances of proteins related to cellular apoptosis, cell structure, and motility. These findings indicated that maternal high fiber intake could alter intestinal morphology, along with the altered intestinal microbiota composition and proteome of offspring.

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Intestinal morphology, immunity and microbiota response to dietary fibers in largemouth bass, Micropterus salmoide

Cited by 69 publications

References 59 publications

Pectin and soybean meal induce stronger inflammatory responses and dysregulation of bile acid (BA) homeostasis than cellulose and cottonseed meal, respectively, in largemouth bass ( Micropterus salmoides ), which might be attributed to their BA binding capacity

Pectin and soybean meal induce stronger inflammatory responses and dysregulation of bile acid (BA) homeostasis than cellulose and cottonseed meal, respectively, in largemouth bass ( Micropterus salmoides ), which might be attributed to their BA binding capacity

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model

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