Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice

Abstract

Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body. Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection. Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them. Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis.

Fecal microbiota transplantation and short-chain fatty acids reduce sepsis mortality by remodeling antibiotic-induced gut microbiota disturbances

“…The epidemiological study indicated that antibiotic use increases intestine bacterial translocation, which may increase vulnerability to systemic infection ( 14 ). However, antibiotics are currently used to treat sepsis ( 15 ), so we hypothesize that the overuse of antibiotics in sepsis patients may result in intestine microecological dysfunction, hence increasing sepsis patient mortality. In the early stages of critical illness, more than half of the commensal microbiota may be lost, making it almost impossible to replenish gut microbiota diversity ( 16 , 17 ).…”

Gut microbiome causal impacts on the prognosis of breast cancer: a Mendelian randomization study

“…Additionally, several published papers indicate a differential abundance of Ruminococcaceae in patients who respond to anti-PD-1 immunotherapy [ 40 , 41 ], as well as of Parabacteroides distasonis [ 42 ]. Desulfovibrio , which showed a causal relationship with total BC and ER-BC, was investigated experimentally in colon cancer [ 43 ] and acute gastrointestinal injury [ 44 ]. Sellimonas , Adlercreutzia , and Rikenellaceae were causally related to total BC and ER + BC.…”

Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

“… 27 Moreover, changes in gut microbiota are associated with the severity of AGI in sepsis. 28 At present, there are few studies on early prediction of sepsis complicated with AGI, and there is a lack of effective biomarkers for early prediction and diagnosis. Herein, at the genus level, 29 gut microbiota species were found to be significantly different between Com-AGI and No-AGI groups, which provides potential research directions for future research.…”