Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

Zuo, Zhigang; Pei, Liu; Liu, Tianzhi; Liu, Xiujuan; Chen, Yuhong; Hu, Zhenjie

doi:10.2147/idr.s440335

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…Furthermore, brain trauma is linked with gut barrier dysfunction, previously known as "leaky" gut [36,37]. Alterations in microbiota diversity are recognized as contributing to infections, and mainly to pneumonia, through the gut-brain-lung axis [38][39][40][41][42], while the disruption of the intestinal barrier, as the result of pathogen overgrowth and loss of the beneficial role of the commensal bacteria, contributes to long-term complications [43][44][45][46] due to the deterioration of host immunity [47].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Probiotics on the Prognostication of the Neutrophil-to-Lymphocyte Ratio in Severe Multi-Trauma Patients

Menni,

Tzikos,

Fyntanidou

et al. 2024

JPM

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Background: The ratio of neutrophils to lymphocytes [NLR] is one of the most accepted prognostic indices and demonstrates a positive correlation with the severity of a disease. Given that probiotics exerted immunomodulatory properties and thus positively affected lymphocytopenia induction in severely ill patients, we performed a post hoc analysis in the ProVAP protocol to investigate whether probiotics affected the prognostication of NLR in respect to ventilator-associated pneumonia in multi-trauma patients. This cohort mandatorily involved severe traumatic brain injury patients. Methods: The white blood cell data of all patients, after being retrieved for the days 0 and 7, were statistically assessed in respect to neutrophils, lymphocytes and NLR among the 4 sub-groups of the study: placebo/no-VAP, placebo/VAP, probiotics/no-VAP, and probiotics/VAP. Results: Lymphopenia was dominant in placebo sub-groups, while an increased level of lymphocytes was prominent in probiotics sub-groups. This resulted in an increase [p = 0.018] in the NLR value in the probiotics/VAP group in relation to the probiotics/no-VAP cohort; this was an increase of half the value of the placebo/VAP [p < 0.001], while the NLR value in placebo/no-VAP group increased almost four-fold in relation to probiotics/no-VAP [p < 0.001]. Additionally, the ROC curve for probiotic-treated patients revealed a NLR7 cut-off value of 7.20 as a prognostic factor of VAP (AUC: 78.6%, p = 0.015, 95% CI: 62.6–94.5%), having a high specificity of 90.2% and a sensitivity of 42.9%. Conclusions: NLR may considered a credible prognostic biomarker in multi-trauma patients since it can evaluate the immunomodulatory benefits of probiotic treatment. However, the results of the present post hoc analysis should be interpreted meticulously until further evaluation, since they may be basically species- or strain-specific.

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Section: Discussionmentioning

confidence: 99%

The Effect of Probiotics on the Prognostication of the Neutrophil-to-Lymphocyte Ratio in Severe Multi-Trauma Patients

Menni,

Tzikos,

Fyntanidou

et al. 2024

JPM

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Global research trends on sepsis and septic shock during 2003–2022: A data-driven bibliometric analysis

Han,

Guo,

Wang

et al. 2024

Preprint

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Background Sepsis is a major medical condition that contributes to substantial morbidity and mortality rates worldwide. Research to better understand and manage these complex conditions has intensified over the past two decades. This bibliometric analysis aims to map global research trends in sepsis and septic shock from 2003 to 2022, providing insights into the evolution of the field. Methods This study conducted a comprehensive search for publications on sepsis and septic shock using the Web of Science Core Collection. Data were analysed using VOSviewer for bibliometric mapping, focusing on publication trends, country, institutional contributions, journal sources, citation analysis, and keyword co-occurrence. Results In total, 78,108 publications were retrieved, with a notable increase in annual output over the study period. The United States ranked first in publication volume, followed by China, Germany, and the United Kingdom. Harvard University was the most prolific institution. Critical Care Medicine emerged as the leading journal in terms of publication count. The most cited articles and references were predominantly related to definition, epidemiology, and management guidelines for sepsis. Recent research hotspots included COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA). Conclusions Our bibliometric analysis identifies global research trends in sepsis over the past 20 years, with evolving hotspots and increasing global collaboration. Key findings highlight continuous efforts to understand the complex pathophysiology of sepsis and septic shock. COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA) are hotspots.

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Exploring the causal relationship between gut microbiota and frailty: a two-sample mendelian randomization analysis

Bo,

Teng,

Shi

et al. 2024

Front. Med.

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BackgroundFrailty is a complex geriatric syndrome that seriously affects the quality of life of older adults. Previous observational studies have reported a strong relationship of frailty with the gut microbiota; however, further studies are warranted to establish a causal link. Accordingly, we aimed to conduct a bidirectional Mendelian randomization study to assess the causal relationship between frailty, as measured by the frailty index, and gut microbiota composition.MethodsInstrumental variables for the frailty index (N = 175, 226) and 211 gut bacteria (N = 18,340) were obtained through a genome-wide association study. A two-sample Mendelian randomization analysis was performed to assess the causal relationship of gut microbiota with frailty. Additionally, we performed inverse Mendelian randomization analyses to examine the direction of causality. Inverse variance weighting was used as the primary method in this study, which was supplemented by horizontal pleiotropy and sensitivity analyses to increase confidence in the results.ResultsBacteroidia (b = −0.041, SE = 0.017, p = 0.014) and Eubacterium ruminantium (b = −0.027, SE = 0.012, p = 0.028) were protective against frailty amelioration. Additionally, the following five bacteria types were associated with high frailty: Betaproteobacteria (b = 0.049, SE = 0.024, p = 0.042), Bifidobacterium (b = 0.042, SE = 0.016, p = 0.013), Clostridium innocuum (b = 0.023, SE = 0.011, p = 0.036), E. coprostanoligenes (b = 0.054, SE = 0.018, p = 0.003), and Allisonella (b = 0.032, SE = 0.013, p = 0.012). Contrastingly, frailty affected Butyrivibrio in the gut microbiota (b = 1.225, SE = 0.570, p = 0.031). The results remained stable within sensitivity and validation analyses.ConclusionOur findings strengthen the evidence of a bidirectional causal link between the gut microbiota and frailty. It is important to elucidate this relationship to optimally enhance the care of older adults and improve their quality of life.

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Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

Cited by 3 publications

References 57 publications

The Effect of Probiotics on the Prognostication of the Neutrophil-to-Lymphocyte Ratio in Severe Multi-Trauma Patients

The Effect of Probiotics on the Prognostication of the Neutrophil-to-Lymphocyte Ratio in Severe Multi-Trauma Patients

Global research trends on sepsis and septic shock during 2003–2022: A data-driven bibliometric analysis

Exploring the causal relationship between gut microbiota and frailty: a two-sample mendelian randomization analysis

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