Intestinal histological alterations in farmed red-bellied pacu Piaractus brachypomus (Characiformes: Serrasalmidae) heavily infected by roundworms

Cuadros, Ruben C.; Rivadeneyra, Norma L. S.; Gonzales, Anai; Mertins, Omar; Malta, José Celso de Oliveira; Serrano‐Martínez, Enrique; Mathews, Patrick D.

doi:10.1007/s10499-021-00670-0

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…After 2, 6 and 24 hr of the first particle administration, three individuals of each aquarium were killed for initial intestinal survey. The fish were individually collected with an appropriate collecting net and immediately killed by neural pithing (Cuadros et al., 2021). In necropsies, the intestines were removed, opened longitudinally, placed on microscopy slides, slightly rinsed with Milli‐Q water using a micropipette and readily examined in a Confocal Leica TCS SP8 Inverted Microscope, using 10×, 40×, 63× and 100× objectives, where the fluorescent dye FITC was excited with a diode‐pumped solid‐state laser at 480 nm and the emission signal was collected at 525 nm.…”

Section: Methodsmentioning

confidence: 99%

High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters

Madrid

Mertins

Tavares‐Dias³

et al. 2021

Journal of Fish Diseases

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Parasite infections in fish require constant surveillance and strategies for efficient treatments which guarantee the fish health, their sale value and the non-propagation of pathogens in new environments. Fish treatments based on nanotechnology become of increasing interest since nanoparticles have been shown as efficient materials for optimizing administration of bioactives. In this study a chitosan derivative, alginate and praziquantel conjugated nanobioparticle of effective action for oral treatment of digenetic trematodes in highly infected Corydoras schwartzi was evaluated in terms of histological and hematological safety. The inherent absence of alterations in intestinal tissue and the reversible blood cells counting during a period up to 35 days showed the safety of the drug delivery nanobioparticles, which thus represent a promising strategy for effective applications in pathogens treatments by oral administration.

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Section: Methodsmentioning

confidence: 99%

High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters

Madrid

Mertins

Tavares‐Dias³

et al. 2021

Journal of Fish Diseases

Self Cite

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show abstract

“…After 2, 6, and 24 h of the first nanoparticle administration, three individuals of each aquarium were sacrificed for initial intestinal examination. In the typical procedure, fish were individually collected with an appropriate collecting net and immediately euthanized by neural pithing . In necropsies, the intestines were removed, opened longitudinally, placed on microscopy slides, rinsed with Milli-Q water using a micropipette, and readily examined in a Confocal Leica TCS SP8 Inverted Microscope, using 10× , 40×, 63×, and 100× objectives, where the fluorescent dye FITC was excited with a diode-pumped solid-state laser at 480 nm and the emission signal was collected at 525 nm.…”

Section: Materials and Methodsmentioning

confidence: 99%

Efficient Treatment of Fish Intestinal Parasites Applying a Membrane-Penetrating Oral Drug Delivery Nanoparticle

Mathews

Patta

Madrid

et al. 2021

ACS Biomater. Sci. Eng.

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Nanodelivery of drugs aims to ensure drug stability in the face of adverse biochemical conditions in the course of administration, concomitant with appropriate pharmacological action provided by delivery at the targeted site. In this study, the application potential of nanoparticles produced with biopolymers chitosan-N-arginine and alginate as an oral drug delivery material is evaluated. Being both macromolecules weak polyelectrolytes, the bioparticle presents strong thermodynamic interaction with a biological model membrane consisting of charged lipid liposome bilayer, leading to membrane disruption and membrane penetration of the bioparticles in ideal conditions of pH corresponding to the oral route. The powder form of the bioparticle was obtained by lyophilization and with a high percentage of entrapment of the anthelmintic drug praziquantel. In vivo studies were conducted with oral administration to Corydoras schwartzi fish with high intensity of intestinal parasites infection. The in vivo experiments confirmed the mucoadhesive and revealed membrane-penetrating properties of the bioparticle by translocating the parasite cyst, which provided target drug release and reduction of over 97% of the fish intestinal parasites. Thus, it was evidenced that the bioparticle was effective in transporting and releasing the drug to the target, providing an efficient treatment.

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Diversity of Nematoda and Digenea from different species of characiform fishes from Tocantins River, Maranhão, Brazil

Cárdenas

Justo

Reyes

et al. 2022

Rev. Bras. Parasitol. Vet.

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During a survey of the helminth fauna of characiform fishes from the Tocantins River, Brazil, 185 fish specimens from 22 species were studied. Twelve species of Nematoda and nine species of Digenea were collected. Some of these helminth species were reported for the first time in their hosts, thus representing new host records: Procamallanus (Spirocamallanus) sp. in Bivibranchia notata, Brycon pesu, Chalceus macrolepidotus, Hemiodus microlepis and Hemiodus unimaculatus; Procamallanus (Spirocamallanus) inopinatus in Triportheus elongatus; Goezia sp. (larva) in Boulengerella cuvieri; Rhabdochona acuminata in Brycon pesu and Triportheus trifurcatus; Raphidascaris sp. (larva) in Caenotropus labyrinthicus; Cosmoxynema vianai in Cyphocharax gouldingi; Rondonia rondoni in Leporinus fasciatus and Mylesinus paucisquamatus; Klossinemella iheringi in Mylesinus paucisquamatus; Cucullanidae gen. sp. in Myloplus rubripinnis; Rhabdochona sp. in Triportheus elongatus; Alphamphistoma sp. in Myleus setiger; Chalcinotrema sp. in Cyphocharax gouldingi; Pacudistoma guianense in Hemiodus unimaculatus and Myleus torquatus; Pseudocladorchis cylindricus in Hemiodus unimaculatus; Dadaytrema oxycephala in Mylesinus paucisquamatus; Travassosinia dilatata in Myloplus asterias; and Genarchella genarchella in Raphiodon vulpinus. Studies identifying new hosts and new localities for parasites have contributed to the knowledge of local biodiversity. A list of previous records of helminths included in the present study, providing hosts, localities, and references, is presented here.

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Intestinal histological alterations in farmed red-bellied pacu Piaractus brachypomus (Characiformes: Serrasalmidae) heavily infected by roundworms

Cited by 4 publications

References 20 publications

High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters

High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters

Efficient Treatment of Fish Intestinal Parasites Applying a Membrane-Penetrating Oral Drug Delivery Nanoparticle

Diversity of Nematoda and Digenea from different species of characiform fishes from Tocantins River, Maranhão, Brazil

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