High compliance and effective treatment of fish endoparasitic infections with oral drug delivery nanobioparticles: Safety of intestinal tissue and blood parameters

Abstract

Parasite infections in fish require constant surveillance and strategies for efficient treatments which guarantee the fish health, their sale value and the non-propagation of pathogens in new environments. Fish treatments based on nanotechnology become of increasing interest since nanoparticles have been shown as efficient materials for optimizing administration of bioactives. In this study a chitosan derivative, alginate and praziquantel conjugated nanobioparticle of effective action for oral treatment of digenetic trematodes in highly infected Corydoras schwartzi was evaluated in terms of histological and hematological safety. The inherent absence of alterations in intestinal tissue and the reversible blood cells counting during a period up to 35 days showed the safety of the drug delivery nanobioparticles, which thus represent a promising strategy for effective applications in pathogens treatments by oral administration.

Effects of Formulation on the Palatability and Efficacy of In-Feed Praziquantel Medications for Marine Finfish Aquaculture

“…The beads of Formulations B and C were shown not to disintegrate in seawater and could be incorporated into fish feed pellets with high palatability for small and large yellowtail kingfish. Unlike the preparation of praziquantel nanoparticles using chitosan-N-arginine and alginate [ 40 , 41 ], the manufacturing processes for these beads are less complex and can easily be up-scaled, and the beads are stable to storage at ambient temperature. More importantly, they were demonstrated to be efficacious against gill parasites, indicating that the incorporated PZQ was released from the beads in vivo for bioactivity in the fish.…”

Cubosomal lipid nanoassemblies with pH-sensitive shells created by biopolymer complexes: A synchrotron SAXS study

“…The described pH-responsiveness, related to the structural features of the biopolymertailored cubosomes, may represent important advantages for the development of colloidal vehicles for biomedical applications where pH variation plays an important role. Previously, the combination of CHarg and ALG in the production of micro and nanoparticles as drug delivery systems has shown great potential for in vivo oral administration [54,[56][57][58][59]. Indeed, the gastrointestinal tract presents extreme pH variation to which drug delivery devices can be developed to respond in a manner to provide localized, optimized, and prolonged delivery of the carried bioactives.…”

“…A recent SAXS study has shown that lipids associated with the polysaccharide chitosan can assemble into certain cubic phases depending on the lipid/polymer mass ratio and the degree of hydration [55]. Moreover, the association of chitosan and chitosan-N-arginine with alginate, another polysaccharide with advantageous characteristics, allowed the production of pH-sensitive nanoparticles as drug delivery systems for the anthelmintic drugs ivermectin, mebendazole and praziquantel [56][57][58][59]. Indeed, chitosan and alginate are oppositely charged polyelectrolytes in aqueous medium, which renders the association of both macromolecules pH-dependent [54].…”

“…Indeed, chitosan and alginate are oppositely charged polyelectrolytes in aqueous medium, which renders the association of both macromolecules pH-dependent [54]. In vivo studies of these nanoparticles in fish animals infected with gastrointestinal parasites have demonstrated the excellent treatment efficacy upon oral administration [54,58,59]. In parallel, the performed physicochemical studies have suggested that the structural changes of the chitosan/alginate nanoparticles, as a function of pH, play a crucial role for the observed in vivo effects [54,58].…”

Interaction of polyelectrolyte-shell cubosomes with serum albumin for triggering drug release in gastrointestinal cancer

“…In this regard, the chemical bonding of arginine on chitosan has provided improved plasmid DNA delivery for liposomes and profitable pH-responsive characteristics to micro and nanoparticles for oral drug delivery. [37][38][39][40][41] Additionally, alginate finds increasing application perspectives in biomedicine and food technology due to its high availability, anionic nature, and especially due to gel forming and pH-dependent properties. 42,43 As opposite charge polyelectrolytes in aqueous solutions, chitosan and alginate have been associated in the production of nanoparticles as complex coacervates intended for antibacterial, antifungal and antitumor applications.…”