Intestinal expression of toll-like receptor gene changes early after gastric bypass surgery and association with type 2 diabetes remission

Sala, Priscila; Torrinhas, Raquel Susana; Fonseca, Danielle Cristina; Machado, Natasha Mendonça; Singer, Joelle; Singer, Pierre; Ravacci, Graziela Rosa; Belarmino, G.; Ferreira, Beatriz de Azevedo Muner; Marques, Mariane; Ishida, Robson Kiyoshi; Guarda, Ismael Francisco Motta Siqueira; Moura, Eduardo Guimarães Hourneaux de; Sakai, Paulo; Santo, Marco Aurélio; Sunaga, Daniele Yumi; Heymsfield, Steven B.; Bezerra, Daniele Pereira dos Santos; Corrêa-Giannella, Maria Lúcia; Waitzberg, Dan Linetzky

doi:10.1016/j.nut.2020.110885

Cited by 7 publications

(9 citation statements)

References 32 publications

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“…104 Many clinical studies have also shown that inflammatory factors and TLR receptors are significantly reduced after surgery, but adipokines like leptin and adiponectin have not shown consistent results. [105][106][107] Some bariatric surgery patients regained weight after weight loss, but the inflammatory factors continued to decrease, indicating that bariatric surgery may have a long-term effect on inflammation control. 108…”

Section: Anti-inflammatory Therapeutic Effect Of Bariatric Surgerymentioning

confidence: 99%

<p>Characterization and Treatment of Inflammation and Insulin Resistance in Obese Adipose Tissue</p>

Song

2020

DMSO

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Adipose tissue is the largest energy storage and protection organ. It is distributed subcutaneously and around the internal organs. It regulates metabolism by storing and releasing fatty acids and secreting adipokines. Excessive nutritional intake results in adipocyte hypertrophy and proliferation, leading to local hypoxia in adipose tissue and changes in the release of adipokines. These lead to recruit of more immune cells into adipose tissue and release of inflammatory signaling factors. Excess free fatty acids and inflammatory factors interfere with intracellular insulin signaling. In this review, we summarize the characteristics of obese adipose tissue and analyze how its inflammation causes insulin resistance. We further discuss the latest clinical research progress on the control of insulin resistance and inflammation resulting from obesity through anti-inflammatory therapy and bariatric surgery. Our review shows that targeted anti-inflammatory therapy is of great significance for obese patients with insulin resistance.

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Section: Anti-inflammatory Therapeutic Effect Of Bariatric Surgerymentioning

confidence: 99%

<p>Characterization and Treatment of Inflammation and Insulin Resistance in Obese Adipose Tissue</p>

Song

2020

DMSO

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“…With the decrease of IL6 after BS, IL10 as the antagonistic anti-inflammatory cytokine of IL6 also decreased [ 40 ]. Abnormal activation of Toll-like receptors (TLRs) had been found to cause obesity-related systemic inflammation and associated comorbidities in obese rats [ 41 ]. Inhibition of TLRs could reduce the activation of MAPKs and NF-κB and ameliorate obesity-induced NAFLD [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease

et al. 2021

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Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.

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“…Here we show that RYGB induces a reduction in intestinal TLR4 expression but not in the liver or any other subtype of WAT. Similarly, a recent study in human subjects showed that RYGB reduces the level of multiple TLRs (including most specifically TLR4) within the duodenum, jejunum, and ileum of diabetic women with obesity [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, extraction of microbial DNA from cecal samples was performed using DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germantown, MD, USA) followed by amplication and seqeuencing of the 16s rRNA V3-V4 region on a Illumina MiSeq platform at Iowa Institute of Human Genetics (IIHG). Raw 16S sequence data was processed by R script Divisive Amplicon Denoising Algorithm 2 (DADA2) to generate amplicon sequence variants (ASVs) which were then assigned taxonomies using a naive Bayesian classifier with the Silva database as a reference [33]. Analysis of these data were then conducted using online microbiome tools METAGENassist [34] and MicrobiomeAnalyst [35].…”

Section: Microbiota Sequencing Analysismentioning

confidence: 99%

Toll-like receptor 4 and myeloid differentiation factor 88 are required for gastric bypass-induced metabolic effects

Haija

Chu

et al. 2021

Surgery for Obesity and Related Diseases

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Intestinal expression of toll-like receptor gene changes early after gastric bypass surgery and association with type 2 diabetes remission

Cited by 7 publications

References 32 publications

<p>Characterization and Treatment of Inflammation and Insulin Resistance in Obese Adipose Tissue</p>

<p>Characterization and Treatment of Inflammation and Insulin Resistance in Obese Adipose Tissue</p>

A novel immune-related genes signature after bariatric surgery is histologically associated with non-alcoholic fatty liver disease

Toll-like receptor 4 and myeloid differentiation factor 88 are required for gastric bypass-induced metabolic effects

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