Intestinal, adipose, and liver inflammation in diet-induced obese mice

Effect of anti-gut inflammatory agent on insulin resistance and lipid profile of mice fed different diets

“…Recently, people proved HFD induced gut inflammation and then triggers metabolic disorders while anti-enteritis agent, 5-ASA reverses IR through improvement of fasting blood glucose, insulin levels and glucose tolerance [21]. These are at variance with the data acquired in this study, which showed that 5-ASA treatment did not have a specific effect on fasting blood glucose levels, fasting plasma insulin levels and GTT response in mice fed either LFD, HFD or HFC.…”

Immunologic impact of the intestine in metabolic disease

“…In the colon, the data are less consistent. Some studies have shown increased TNF-α, but not IL-6, at the mRNA level after short-and long-duration HFD feeding (74,75), while other studies have reported no differences (73,76). After ten or 30 days of HFD feeding, intestinal APCs (MHC2 + CD19 -) show reduced activation markers such as CD86 and a reduced ability to induce Th17 cells in vitro, but also show an upregulation of certain inflammatory gene such as Nlrp3 (49).…”

In vivo imaging of lipid storage and regression in diet-induced obesity during nutrition manipulation

“…The first group was given a regular R3 chow diet for 16 wk (low-fat diet group, R3). The second group was fed a cafeteria (CAF) diet, consisting of cheese (containing 38% fat, 1% carbohydrate, and 20% protein; Arla Ost, Västervik, Sweden), chocolate (containing 32% fat, 58% carbohydrate, and 6% protein; Marabou, Kraft Foods, Upplands Väsby, Sweden), nougat (containing 39% fat, 47% carbohydrate, and 6% protein; Odense Marcipan, Odense, Denmark), and chocolate pastry (containing 31% fat, 52% carbohydrate, and 5% protein; Delicatoboll, Delicato, Huddinge, Sweden), together with regular chow (R3) for 16 wk (CAF) (30). The third group was fed the CAF diet for 12 wk and then transferred to the regular R3 chow diet for the remaining 4 wk (weight-reduced group, WR).…”

“…The livers of the remaining animals (n ϭ 5/group) were dissected and weighed, and samples were taken for liver triglyceride (TG) content as described in Ref. 30. Parametrial WAT was dissected by opening the abdominal cavity of the mouse, locating the uterus, and dissecting the fat pads from the wall of the uterus and both uterine horns.…”